FastQCFastQC Report
Tue 22 Apr 2025
Site5z_S131_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5z_S131_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515182
Total Bases101.8 Mbp
Sequences flagged as poor quality0
Sequence length17-281
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA7432314.426552169912767No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG369537.172804950483519No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG266425.171376329141935No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG193973.7650771960200475No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC136282.6452787558571536No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG102191.9835708545717825No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA61991.203264089195663No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG52881.0264333769425174No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC49790.9664545733352484No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG43570.8457205414785455No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA36060.6999468149120116No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA26910.5223396780167009No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA26350.5114697330263868No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA24110.46798995306513036No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT22180.43052746408065495No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT21410.4155812897189731No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG21100.4095639987421921No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA20840.40451723856811767No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT19650.3814186054637002No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT18730.36356083869389844No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT18290.35502016763008026No Hit
CTACGGAAACCTTGTTACGACTTCAGCTTCTTCTAAATAAATAGGTTTAA17880.34706181504788597No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT17820.34589717808463805No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA16570.3216339080169726No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT16150.3134814492742371No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA15900.30862879526070397No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT15900.30862879526070397No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT15440.2996999118758031No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT14850.288247648403865No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT14790.2870830114406171No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT14390.2793187650189642No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG13430.26068457360699715No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT13380.25971404280429056No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT12700.24651482388748053No Hit
GAATCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT12520.24302091299773673No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT12520.24302091299773673No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT12400.24069163907124083No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA12220.237197728181497No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT12140.23564487889716643No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT12120.2352566665760838No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA11730.2276865263149722No Hit
AAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT11320.21972817373277792No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT11300.2193399614116953No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT11210.21759300596682338No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT11210.21759300596682338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11100.2154578382008688No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT11080.21506962587978617No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG10780.20924644106354648No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT10640.20652895481596797No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG10570.20517021169217867No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA10530.20439378705001338No Hit
GAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT10450.20284093776568282No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT10430.20245272544460016No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT10320.20031755767864562No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGTTGATCAAGTTTGA10290.19973523919702163No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT10120.19643543446781916No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC9970.1935238420596993No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA9930.19274741741753398No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC9750.1892535065277902No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT9640.18711833876183562No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT9550.1853713833169637No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT9480.18401264019317445No Hit
GAACCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT9120.1770248184136868No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG9060.17586018145043886No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT8880.17236627056069506No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA8850.17178395207907107No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT8620.16731951038662066No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC8110.15742009619901318No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT7930.15392618530926933No Hit
AAATCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT7850.15237333602493877No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT7670.14887942513519492No Hit
AAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7550.14655015120869905No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7490.14538551424545113No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTCCAGATTTAA7430.14422087728220317No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT7320.1420857095162486No Hit
GAATCAAAATAAATGCTGGTAAAGCACAGGATCTCCTCCTCCAGCGGGAT7200.13975643558975273No Hit
AAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT7060.1370389493421742No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA7030.13645663086055024No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT6840.1327686138102651No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT6650.12908059675997996No Hit
GAACCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT6590.12791595979673204No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT6580.1277218536361907No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC6570.12752774747564938No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT6390.12403383658590557No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGCAATACAGTTCGA6380.12383973042536425No Hit
GAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6370.12364562426482291No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6350.12325741194374026No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT6270.12170456265940968No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG6210.12053992569616173No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGATTTAC6170.11976350105399645No Hit
AAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6160.11956939489345512No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6090.11821065176966586No Hit
AAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT5940.11529905936154602No Hit
AAACCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT5920.11491084704046337No Hit
GAACCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT5880.11413442239829807No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAA5840.11335799775613278No Hit
AAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5790.11238746695342618No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA5780.11219336079288485No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT5740.11141693615071954No Hit
GAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT5640.10947587454530631No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGAAATTCAGTTTGA5580.10831123758205839No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT5350.10384679588960795No Hit
TACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGAT5320.10326447740798399No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTACATGATCAAGTTTGA5240.10171162812365338No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5240.10171162812365338No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT5230.10151752196311206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)