FastQCFastQC Report
Tue 22 Apr 2025
Site5w_S128_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5w_S128_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Total Bases2.5 kbp
Sequences flagged as poor quality0
Sequence length141-281
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC215.384615384615385No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG215.384615384615385No Hit
AAATCAGAATAGGTGTTGGTATAGAACTGGATCTCCTCCCCCAGCAGGGT17.6923076923076925No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT17.6923076923076925No Hit
AAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT17.6923076923076925No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC17.6923076923076925No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT17.6923076923076925No Hit
GAATCAAAATAAATGTTGATACAATACAGGGTCTCCTCCTCCTGCCGGAT17.6923076923076925No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA17.6923076923076925No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAAACATGAGATTTAA17.6923076923076925No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)