FastQCFastQC Report
Tue 22 Apr 2025
Site5w_S128_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5w_S128_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Total Bases2.5 kbp
Sequences flagged as poor quality0
Sequence length141-281
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG215.384615384615385No Hit
TCCCCCTTTGAGCAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG17.6923076923076925No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT17.6923076923076925No Hit
CCCCCCATTAGCAAGTATTGCTTTCCATTCAGGAGGAGCGGTTGATTGTG17.6923076923076925No Hit
CCCCCCATTAAGTAGCGCTCAAGCTCACTCAGGACCGTCGGTAGATTTAG17.6923076923076925No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG17.6923076923076925No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT17.6923076923076925No Hit
CTACCGATTGAGTGATCCGGTGAATTGTTCGGACTGCGGCCATTTTCGGT17.6923076923076925No Hit
CCCCCCTCTTGCAAGTATTCAGTTCCACTCTGGTGGTGCTGTTGATTGTG17.6923076923076925No Hit
CTACCGATTGAAACATACGGTGAAAATCTCGGAGAGAAAAGTTCTTGGTG17.6923076923076925No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTTGTCTGGCGGTT17.6923076923076925No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)