FastQCFastQC Report
Tue 22 Apr 2025
Site5v_S127_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5v_S127_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82674
Total Bases14.4 Mbp
Sequences flagged as poor quality0
Sequence length44-281
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG948911.477610857101386No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG44625.397101869995404No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG38544.661683237777294No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG31523.8125650143938845No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG30203.6529017587149526No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA24242.9319979679222006No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA22682.7433050293925536No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG17652.134891259646322No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC17502.116747707864625No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA16331.97522800396739No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC14411.7429905411616713No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG10771.3027070179258293No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC9361.1321576311778794No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA9051.094660957495706No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA8751.0583738539323124No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGC8240.9966857778745434No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA7950.9616082444299296No Hit
CTACGGAAACCTTGTTACGACTTCAGCTTCTTCTAAATAAATAGGTTTAA7800.9434646926482328No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG6170.7463047632871278No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT4960.5999467789147738No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4390.531001282144326No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG4190.5068098797687302No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT4190.5068098797687302No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT3940.47657062679923556No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA3760.45479836466119944No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGCATCAGGATTTAA3760.45479836466119944No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA3570.4318165324043835No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT3510.4245591116917048No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3380.40883470014756756No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGCTCAA3350.4052059897912282No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3270.3955294288409899No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT3220.389481578247091No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3070.3713380264653942No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATAAAACAGTTTGG3070.3713380264653942No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3040.36770931610905483No Hit
GAATCAGAAGAGATGTTGGAAAAGAATAGGGTCTCCTCCTCCTGCTGGGT2790.3374700631395602No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT2710.3277935021893219No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAACAAGTTTGA2700.32658393207054215No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA2680.3241647918329826No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATATGGTTAAC2660.321745651595423No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT2660.321745651595423No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA2610.31569780100152406No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2560.3096499504076251No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA2520.30481166993250597No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG2490.30118295957616664No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG2490.30118295957616664No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG2450.2963446791010475No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2450.2963446791010475No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2430.2939255388634879No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA2410.2915063986259283No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2400.29029682850714855No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC2400.29029682850714855No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTGA2360.2854585480320294No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2310.2794106974381305No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2300.2782011273193507No Hit
AAATCAGAAGAGATGTTGGAAAAGAATAGGGTCTCCTCCTCCTGCTGGGT2280.27578198708179114No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCCAGTAGAAAAGGCTCAT2270.2745724169630114No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG2140.25884800541887415No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG2110.25521929506253477No Hit
CTACGGAAACCTTGTTACGACTTTTACTATCTCTAAATGAACAAGTTTGA2090.2528001548249752No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2060.24917144446863584No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG2050.24796187434985606No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2040.24675230423107627No Hit
CTACGATAACCTTGTTACGACTTTTTCTTCCTCCAATACAAAGCATTCGC1980.23949488351839754No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1960.237075743280838No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT1940.23465660304327843No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC1920.23223746280571886No Hit
AAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1880.22739918233059972No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT1860.22498004209304012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1800.21772262138036141No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCTTCTAGCTGCAGAATATTGA1790.21651305126158166No Hit
GAACCAGAAGAGATGTTGGAAAAGAATAGGGTCTCCTCCTCCTGCTGGGT1760.21288434090524228No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1750.2116747707864625No Hit
AAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1700.2056269201925636No Hit
AAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1650.19957906959866462No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT1610.1947407891235455No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG1510.18264508793574766No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1390.16813024651039019No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT1390.16813024651039019No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAA1350.16329196603527107No Hit
CTACAGCAACCTTGTTACGACTTTTTCTTCCTCCAATATTCAATGTTTAC1320.15966325567893172No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT1280.15482497520381255No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1270.15361540508503277No Hit
CTACGGAAACCTTGTCACGACTTCACCTTCCTCTAAATGATGAGGTTTAA1270.15361540508503277No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA1260.15240583496625298No Hit
AAACCAGAAGAGATGTTGGAAAAGAATAGGGTCTCCTCCTCCTGCTGGGT1230.14877712460991366No Hit
GAATCAAAATAAATGCTGGTAGAGAACCGGGTCACCCCCTCCTCCAACGT1220.14756755449113385No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATAAGGTTTAG1210.14635798437235406No Hit
GAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1150.13910056365967535No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1130.13668142342211578No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTTCTAAATGATGAGGTT1120.13547185330333597No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA1050.1270048624718775No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA1020.12337615211553814No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA1020.12337615211553814No Hit
AAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1010.12216658199675834No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTCGA970.11732830152163921No Hit
CTACGATAACCTTGTTACGACTTTTTCTTCCTCCAATACGAAGCATTCGC950.11490916128407964No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC950.11490916128407964No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC950.11490916128407964No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGA930.11249002104652006No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT930.11249002104652006No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG920.11128045092774028No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAC900.10886131069018071No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA900.10886131069018071No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA880.10644217045262114No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT870.10523260033384134No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA870.10523260033384134No Hit
AAATCAAAATAAATGCTGGTAGAGAACCGGGTCACCCCCTCCTCCAACGT840.101603889977502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)