FastQCFastQC Report
Tue 22 Apr 2025
Site5v_S127_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5v_S127_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82674
Total Bases14.4 Mbp
Sequences flagged as poor quality0
Sequence length53-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT53366.454266153808937No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGCCTGGCGGAT38824.695551201103128No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT31053.7557152188112344No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT30373.6734644507342087No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT26093.1557684398964607No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT25113.037230568256042No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG24953.017877446355565No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT24682.985219053148511No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG20432.4711517526671023No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT18892.2848779543750153No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG17002.0562692019256357No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG14551.759924522824588No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG13221.5990516970268769No Hit
CTACCGATTGAACGTTTTAGTGAGGTGTTTGGACCTGGCCTGGAGGGGCT11871.4357597309916057No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA11561.3982630573094323No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT11161.3498802525582407No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC10891.3172218593511866No Hit
CTACCGATTGAATGGCTTAGTGAGCCTTTGGGACTAGCCGCTTGTACCGG8451.0220867503689188No Hit
CTACTGATTGAATACATCGGTGAAATATTGGGAATGTGATTTTTAAAGCT8010.9688656651426084No Hit
CTACCGATTGAATGGCTTAGTGAGATTTCCGGATTGGCGTTAGGAAGCCG7720.9337881316979945No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG7470.9035488787284999No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT6160.7450951931683479No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG5780.6991315286547162No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT5400.6531678641410843No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTACTTGGTGGGGCT5160.6241381812903694No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA5130.6205094709340301No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGCCTTGCCTTG4870.5890606478457556No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT4330.5237438614316472No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT4300.5201151510753078No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG4160.5031811694123909No Hit
CTACCGATTGAGCATTAGGGTGAAATCTTCGGACCGTGGCATACTTCTGG4150.501971599293611No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT4030.48745675786825365No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG3790.45842707501753877No Hit
CTACCGATTGGATGGTCCGGTGAAATGTTCGGATTCAGTGGTCTACGCGG3780.45721750489875895No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC3680.4451218037109611No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3320.40157727943488886No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG3260.3943198587222101No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG3160.38222415753441225No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGACCCGTTTTGGAG3140.3798050172968527No Hit
CTACCGATTGAATGAATTAGTGAGGCCTTTGGATTTAGAAGCGCTTTTTC3080.37254759658417397No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA2950.35682318504003674No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG2940.355613614921257No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGTGGCTCGCTGACGG2840.34351791373345913No Hit
TCCTCCTTTATCCGCCAACATCGCTCACGCTGGCACAAGAATCGATCTCT2840.34351791373345913No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG2790.3374700631395602No Hit
CTACCGATTGAATGATTCGGTGAATTATCTGGACTGTTTTGATCTTTAAG2770.3350509229020006No Hit
CTACCGATCGAGTGGATTAGTGAGGACCTCGGATTGGCGATTTGGCAGCG2630.31811694123908363No Hit
CTACCGATTGAATGGTCCGGTGAGATCCTCGGACTGCGACGACAATCTGG2620.3169073711203038No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG2600.31448823088274425No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATCGGACCTCTGGAGGTT2570.3108595205264049No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2570.3108595205264049No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT2520.30481166993250597No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTACTTGGTGGGCTT2470.2987638193386071No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2450.2963446791010475No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2450.2963446791010475No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA2440.2951351089822677No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2370.2866681181508092No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT2230.2697341364878922No Hit
TCCCCCTTTATCCGCCAACATCGCTCACGCTGGCACAAGAATCGATCTCT2220.2685245663691124No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG2210.2673149962503326No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATTGTGGTCTGTTTCCTT2150.2600575755376539No Hit
CTACCGACTGGTAGATATGGTGAATGGATTGGACTGAGATTTAGTCGGCT2150.2600575755376539No Hit
CTACCGATTGAGTGGATTAGTGAGGACATCGGATTGAGAAATTGTTGGTG2140.25884800541887415No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTTAGT2140.25884800541887415No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG2040.24675230423107627No Hit
CTACTGATTGGCTGTAGGGTAGAGATCGGCGGATTTCGGCTCATTGGGGC1980.23949488351839754No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG1960.237075743280838No Hit
ATACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT1940.23465660304327843No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT1800.21772262138036141No Hit
CCCTCCTTTATCCGCCAACATCGCTCACGCTGGCACAAGAATCGATCTCT1710.20683649031134335No Hit
CCCCCCTTTATCCGCCAACATCGCTCACGCTGGCACAAGAATCGATCTCT1660.20078863971744443No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGACCGTGACTTTGATTTGT1640.19836949947988486No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTTCGGAGGCT1480.17901637757940828No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT1450.17538766722306892No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1430.17296852698550935No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG1350.16329196603527107No Hit
CTACCAATTGAATGATTAGGTGAAAATCTCGGACTTTGGCATGGAACTTT1330.1608728257977115No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT1300.15724411544137212No Hit
TCCTCCGTTAAGGGGAAACCTGGCCCACAGTGGGAGGTCAGTTGATTTCG1290.15603454532259234No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATCGTGGCGAGTTTCCTT1260.15240583496625298No Hit
CTTCCGACTGATACTCATTGTGAGTTGCAAGGATTCATTTTTGAAGAATT1260.15240583496625298No Hit
CTACCGATTGAATCATTCGGTGAGGATCTCGGATTTTGTTGCTTACGCTT1180.1427292740160147No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTGCCTTT1120.13547185330333597No Hit
CTTCCGACTGATATTCAATGTGAATTGTGAGGGATTTTGTGAATCATTGT1100.13305271306577643No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT1070.12942400270943707No Hit
CCCTCCGTTAAGGGGAAACCTGGCCCACAGTGGGAGGTCAGTTGATTTCG1050.1270048624718775No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTTG1050.1270048624718775No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT970.11732830152163921No Hit
CTACCGATTGGATGGTCCGGTGAAGCCTCGGGATTGTGACCGGTGCCTTT930.11249002104652006No Hit
TCCCCCGTTAAGGGGAAACCTGGCCCACAGTGGGAGGTCAGTTGATTTCG920.11128045092774028No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCTGGTTTCCTT900.10886131069018071No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT870.10523260033384134No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTTGGACTGTGACATTGCTTTGC860.10402303021506155No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT840.101603889977502No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGGCGCCATCGTGGCT840.101603889977502No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT840.101603889977502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)