FastQCFastQC Report
Tue 22 Apr 2025
Site5u_S126_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5u_S126_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86372
Total Bases16.3 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG971611.249015884777474No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC48815.651136942527671No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC39254.544296762839809No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA34744.0221368035937575No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG23172.6825823183439077No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG19342.2391515768999213No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG17442.0191728801000326No Hit
GAATCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT17131.9832816190432083No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG17091.9786504885842635No Hit
AAATCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT12681.468068355485574No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATGTGGTTTAC10571.2237762237762237No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT9641.116102440605752No Hit
GAACCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT9581.1091557449173344No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA9301.0767378317047192No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA9181.062844440327884No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG8951.03621544018895No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA7530.8718103088964017No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG7170.8301301347658963No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA6930.8023433520122261No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6910.8000277867827538No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA6640.7687676561848747No Hit
AAACCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT6450.7467697865048859No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT6370.7375075255869958No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC6100.7062473949891169No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT5930.6865650905386005No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC5860.6784606122354467No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC5630.6518316120965127No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG5280.6113092205807438No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT5060.5858380030565461No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTAAC4930.5707868290649748No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTCGA4620.5348955680081509No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT4590.531422220163942No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG4150.4804797851155467No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG4150.4804797851155467No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA4130.4781642198860742No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4120.47700643727133796No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG4110.47584865465660164No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT4100.47469087204186544No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGCTTAT3600.41680174130505254No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3600.41680174130505254No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT3500.40522391515769No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA3500.40522391515769No Hit
GAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT2760.319548001667207No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2740.31723243643773447No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA2730.3160746538229982No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTGC2600.30102347983142685No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2520.2917612189135368No Hit
GAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT2480.28713008845459176No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC2450.28365674061038304No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGCTCAA2410.279025610151438No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2370.274394479692493No Hit
GAATCAGAAAAGATGCTGATAAAGAACTGGATCTCCTCCCCCGGCAGGGT2350.27207891446302046No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATGTGGTTTAC2340.27092113184828415No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTTGT2330.26976334923354794No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2260.2616588709303941No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2170.2512388273977678No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC2100.24313434909461398No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC2050.23734543602093272No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT1910.22113647941462514No Hit
AAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT1850.2141897837262076No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA1800.20840087065252627No Hit
GAATCAAAATAAGTGTTGATACAGAACTGGATCACCCCCTCCGGTAGGGT1790.20724308803779004No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1770.20492752280831752No Hit
AAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT1690.1956652618904275No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTACGATTTAT1670.19334969666095495No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATGTGGTTTAC1570.18177187051359237No Hit
GAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT1510.17482517482517482No Hit
AAATCAGAAAAGATGCTGATAAAGAACTGGATCTCCTCCCCCGGCAGGGT1490.1725096095957023No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT1450.16787847913675727No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1410.16324734867781227No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG1390.16093178344833975No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC1380.1597740008336035No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA1360.15745843560413098No Hit
GAACCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT1330.1539850877599222No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT1310.15166952253044969No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG1300.15051173991571343No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA1260.1458806094567684No Hit
GAACCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT1240.14356504422729588No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTCTAGGTTCAT1230.14240726161255962No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATGTGGTTTAC1210.1400916963830871No Hit
AAACCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT1170.13546056592414207No Hit
AAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT1150.13314500069466956No Hit
GAACCAAAATAAGTGTTGATACAGAACTGGATCACCCCCTCCGGTAGGGT1150.13314500069466956No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTAGACAAGTTCGA1130.13082943546519704No Hit
AAATCAAAATAAGTGTTGATACAGAACTGGATCACCCCCTCCGGTAGGGT1120.12967165285046078No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1110.12851387023572455No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG1100.1273560876209883No Hit
AAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT1060.12272495716204325No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC980.1134626962441532No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT970.11230491362941694No Hit
CTACGAAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGATTCAC950.10998934839994443No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAG930.10767378317047191No Hit
GAACCAGAAAAGATGCTGATAAAGAACTGGATCTCCTCCCCCGGCAGGGT920.10651600055573565No Hit
GAATCAGAAAAGATGCTGGTAAAGAACAGGATCACCACCGAAGATTGGAT920.10651600055573565No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACTGAGTTTGA890.10304265271152688No Hit
GAACCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT880.10188487009679062No Hit
AAACCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT880.10188487009679062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)