FastQCFastQC Report
Tue 22 Apr 2025
Site5u_S126_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5u_S126_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86372
Total Bases16.3 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT1010311.697077756680406No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT36194.190015282730514No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG24342.81804288426805No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT20722.3989255777335248No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT17932.0759042282221087No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT17221.9937016625758346No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA13911.6104756170981338No Hit
TCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG12021.391654702912981No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG12011.390496920298245No Hit
TCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG11501.3314500069466957No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGTGCCTCGA11071.2816653545130368No Hit
CCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG9481.097577918769972No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG8080.9354883527068958No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG7700.891492613346918No Hit
CCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG7530.8718103088964017No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT7530.8718103088964017No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA7390.855601352290094No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG7100.8220256564627425No Hit
CTACCGATTGAATGGTCCGGTGAAATTTTCGGACTGCGGCAATGTCGCCG6960.805816699856435No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT6820.7896077432501274No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT6520.7548742648080397No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT6260.724771916824897No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG6230.7212985689806881No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA6120.7085629602185893No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGCT5850.6773028296207104No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5750.6657250034733478No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT5690.6587783077849303No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT5650.6541471773259853No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG5620.6506738294817765No Hit
CTACCGATTGAATGGCTTAGTGAGATTTCCGGATTGGCGTTAGGAAGCCG5460.6321493076459964No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5310.6147825684249525No Hit
CTACCGATTGAGCAATCCGGTGAATATTTCGGACCGGACCCTGTCGGGGC5180.5997313944333812No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4820.558051220302876No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCCCGCCC4660.5395266984670959No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4560.5279488723197332No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4550.526791089704997No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG4420.5117399157134257No Hit
CTACCGATTGGATGGTCCGGTGAATTGATCGGACTGAAGAATATTTTCGC4040.4677441763534479No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGACGGTGCTCG4020.4654286111239754No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT3960.45848191543555783No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3780.43764182837030524No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3600.41680174130505254No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT3470.4017505673134812No Hit
CTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTC3250.37627934978928357No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3180.36817487148612976No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCTCGTTGTTGG3130.3623859584124485No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3120.3612281757977122No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC3000.3473347844208771No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2820.32649469735562453No Hit
CTACCGATTGAATGGCTTAGTGAGACCTCCGGATTGGCGTTGAGAAGCCG2760.319548001667207No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG2750.3183902190524707No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT2680.3102857407493169No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2660.3079701755198444No Hit
CTACCGATTGAATGGCTTAGTGAGGTTCAAGGAGTGCGTCATCCAGTTGG2570.2975501319872181No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG2520.2917612189135368No Hit
CTACCGATTGGATGGTCCGGTGAAATGTTCGGATCCAAGAGTTTACGCGG2380.2755522623072292No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA2350.27207891446302046No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGAATTAGGTTAGTT2350.27207891446302046No Hit
TCCTCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG2330.26976334923354794No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT2270.2628166535451304No Hit
CTACCGATTGAATGATCCGGTGAGCTTTTTGGACTGTGGCACCGGTTGTG2230.25818552308618536No Hit
ATACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT2220.2570277404714491No Hit
TCCTCCATTAGCAAGTATTGCTTTCCATTCAGGAGGAGCGGTTGATTGTG2140.24776547955355901No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT2100.24313434909461398No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG2050.23734543602093272No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2030.2350298707914602No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA2030.2350298707914602No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCGT1990.2303987403325152No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG1950.22576760987357017No Hit
TCCCCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG1930.22345204464409765No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC1860.21534756634094382No Hit
TCCTCCATTAGCAAGTATTGCATTCCACTCAGGGGGTGCAGTTGATTGTG1850.2141897837262076No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1840.2130320011114713No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC1830.21187421849673507No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1810.20955865326726256No Hit
TCCCCCATTAGCAAGTATTGCTTTCCATTCAGGAGGAGCGGTTGATTGTG1790.20724308803779004No Hit
CCCTCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG1760.20376974019358124No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGAGAGTAGCGTACCTTG1680.1945074792756912No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT1660.1921919140462187No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG1530.17714074005464733No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1500.17366739221043856No Hit
TCCCCCATTAGCAAGTATTGCATTCCACTCAGGGGGTGCAGTTGATTGTG1500.17366739221043856No Hit
TCCTCCTCTTTCTAGAAATATTGCTCATGGGGGCCCAGCTGTTGATATGG1470.17019404436622979No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG1450.16787847913675727No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1430.16556291390728478No Hit
TCCTCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG1410.16324734867781227No Hit
CCCCCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG1410.16324734867781227No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC1400.162089566063076No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG1390.16093178344833975No Hit
CCCCCCATTAGCAAGTATTGCTTTCCATTCAGGAGGAGCGGTTGATTGTG1360.15745843560413098No Hit
CCCTCCATTAGCAAGTATTGCTTTCCATTCAGGAGGAGCGGTTGATTGTG1350.15630065298939472No Hit
CTACCGATTGAGTGTTCCGGTGAATTGTCTGGATCAGCTGTCTAGTCGGG1350.15630065298939472No Hit
TCCCCCTCTTTCTAGAAATATTGCTCATGGGGGCCCAGCTGTTGATATGG1350.15630065298939472No Hit
CCCCCCATTAGCAAGTATTGCATTCCACTCAGGGGGTGCAGTTGATTGTG1340.15514287037465846No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1330.1539850877599222No Hit
CCCTCCATTAGCAAGTATTGCATTCCACTCAGGGGGTGCAGTTGATTGTG1290.14935395730097717No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGCTTGGT1290.14935395730097717No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG1290.14935395730097717No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1260.1458806094567684No Hit
TCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG1260.1458806094567684No Hit
TCCCCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG1220.14124947899782336No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT1180.13661834853887833No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1170.13546056592414207No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGCGTCTCGT1170.13546056592414207No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG1150.13314500069466956No Hit
CTACCGATTGAGTGGATTAGTGAGATCCCCGGATCGGCGCCGACATGATG1140.1319872180799333No Hit
CTACCGATTGAGTGCTCAGGTGAATAGTATGGACTGCATCAGTGTTCGGT1080.12504052239151578No Hit
TCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTGGACTTTG1050.12156717454730699No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT1050.12156717454730699No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1030.11925160931783449No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG1020.11809382670309823No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCGCGTGGCTTCG1020.11809382670309823No Hit
TCCCCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG1010.11693604408836197No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA970.11230491362941694No Hit
TCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG970.11230491362941694No Hit
TCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTGGACTTTG940.10883156578520817No Hit
CCCTCCTCTTTCTAGAAATATTGCTCATGGGGGCCCAGCTGTTGATATGG930.10767378317047191No Hit
CCCCCCTCTTTCTAGAAATATTGCTCATGGGGGCCCAGCTGTTGATATGG930.10767378317047191No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG930.10767378317047191No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT910.1053582179409994No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCCTCG890.10304265271152688No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG880.10188487009679062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)