FastQCFastQC Report
Tue 22 Apr 2025
Site5s_S124_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5s_S124_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132316
Total Bases24.3 Mbp
Sequences flagged as poor quality0
Sequence length44-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG127249.616372925420961No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG91476.912996160706188No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC68265.158862117960035No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG58054.38722452311134No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG51113.862722573233774No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG28332.141086489918075No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC26732.020163850176849No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT26141.9755736267722726No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG26111.9733063272771245No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA17291.3067202757036185No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG15741.1895764684543064No Hit
GAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT14371.086036458175882No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC14001.0580730977357236No Hit
AAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT10700.8086701532694458No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT10700.8086701532694458No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA10320.7799510263309049No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAGGTTTGT8980.6786783155476284No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA8930.6748994830557151No Hit
GAACCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT8460.6393784576317301No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT7590.5736267722724387No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA7570.5721152392756733No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6610.4995616554309381No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTTAC6510.49200399044711146No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC6250.4723540614891623No Hit
CTACGGAAACCTTGTTACAACTTCACCTTCCTCTAAATGATGAGGTTTAA6110.461773330511805No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT5950.44968106653768253No Hit
AAACCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT5410.40886967562501886No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA5400.40811390912663625No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA4940.3733486502010339No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT4900.3703255842075032No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTCAGATTTAT4570.3453852897608755No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC4400.33253725928837025No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC4390.3317814927899876No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATTCAGTTTGG4210.3181776958190997No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3910.29550470086761993No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGATTTAC3760.28416820339188004No Hit
GAATCAAAATAAATGTTGGTATAAAACCGGATCTCCTCCTCCACTAGCGT3740.2826566703951147No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT3590.2713201729193748No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3360.25393754345657366No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTAAC3220.24335681247921642No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATTATAAGGTTCGG3180.24033374648568576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3150.23806644699053778No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA3010.22748571601318057No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG2950.22295111702288462No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2920.22068381752773664No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATAAGGTTTGG2870.2169049850358233No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA2820.21312615254391004No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTAAGGTTCAC2790.21085885304876206No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG2760.20859155355361408No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT2700.20405695456331813No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG2670.20178965506817015No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC2650.2002781220714048No Hit
AAATCAAAATAAATGTTGGTATAAAACCGGATCTCCTCCTCCACTAGCGT2630.1987665890746395No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT2540.19196469058919555No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAA2460.18591855860213427No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCTCCTCTTGCAT2450.1851627921037516No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG2330.17609359412315972No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2240.16929169563771576No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTCTGG2120.16022249765712385No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA2050.15493213216844523No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2010.1519090661749146No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG1930.14586293418785332No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT1930.14586293418785332No Hit
AAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT1860.1405725686991747No Hit
GAACCAAAATAAATGTTGGTATAAAACCGGATCTCCTCCTCCACTAGCGT1810.1367937362072614No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA1800.13603796970887874No Hit
GAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT1800.13603796970887874No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATGTGGTTTAC1790.13528220321049608No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAG1790.13528220321049608No Hit
CTACGGTAACCTTGTTACGACTTCACCTTCCTCTAAAGGTAAGGATTTGC1770.13377067021373076No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCTCCTCTTGCAT1760.1330149037153481No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG1660.1254572387315215No Hit
GAATCAAAATAAATGTTGGTATAAAATTGGATCACCTCCACCTGCGGGAT1610.12167840623960821No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1600.12092263974122555No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA1510.11412074125578163No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC1490.1126092082590163No Hit
GAATCAGAATAAATGCTGGTAGAGAATAGGGTCTCCACCTCCAGCAGGGT1480.11185344176063362No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT1460.11034190876386832No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC1450.10958614226548564No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGATAAGGTTTAC1450.10958614226548564No Hit
GAACCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT1420.10731884277033767No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT1420.10731884277033767No Hit
CTACAGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC1400.10580730977357235No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCTCCTCTTGCAT1400.10580730977357235No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGT1390.1050515432751897No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG1350.10202847728165906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)