FastQCFastQC Report
Tue 22 Apr 2025
Site5s_S124_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5s_S124_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132316
Total Bases24.3 Mbp
Sequences flagged as poor quality0
Sequence length52-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT1361110.28673780948638No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT93417.059614861392424No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT51583.898243598657759No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG35622.6920402672390336No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT31032.345143444481393No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA27112.0488829771153902No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT26151.9763293932706552No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT21941.6581516974515553No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT20231.5289156262281205No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG18681.4117718189788082No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTG17691.3369509356389249No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT16421.2409685903443273No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT16011.209982163910638No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG15511.1721938389915052No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG14761.1155113516128057No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG12720.961334985942743No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG12300.9295927930106714No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG12120.9159889960397836No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG10740.8116932192629764No Hit
TCCTCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG10640.80413555427915No Hit
CTACCGATTGGACGTTTTGGTGAGGCTCATGGATCACCGCCCTTTGTGGT9620.7270473714441186No Hit
TCCCCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG8860.6696091175670364No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG8600.6499591886090873No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGGCCTGTCTTGGTT8330.6295534931527555No Hit
CTACCGATTGAATGGCTTAGTGAGATTTCCGGATTGGCGTTAGGAAGCCG8210.6204842951721636No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG7900.5970555337223011No Hit
CCCTCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG7660.5789171377611173No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG7350.5554883763112549No Hit
CCCCCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG7170.541884579340367No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG6900.5214788838840352No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA6850.5177000513921219No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT6830.5161885183953565No Hit
CTACCGATTGAGCAATCCGGTGAATATTTCGGACCGAATAGTTGCGGGGT6400.4836905589649022No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT6170.466307929502101No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGCT6100.4610175640134224No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT6030.4557271985247438No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5640.4262523050878201No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT5600.4232292390942894No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT4920.37183711720426854No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG4870.36805828471235524No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTTG4520.34160645726896216No Hit
CTACCGATTGAATGTTTTGATGAATTGCGCGGACCGGCTGACCTGGGTGG4470.3378276247770489No Hit
CTACCGATCGAACGATGTAGTGAGGTCCTCGGACTGGCATCTACTCGGAA4430.3348045587835182No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG4330.32724689379969163No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTTTCGTTGG4150.31364309682880376No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG4110.3106200308352731No Hit
CTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACAGCGGTGAGCTTCG4070.3075969648417425No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG4040.3053296653465945No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG4000.3023065993530639No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGATTGGACGTCGAGAGGTT3840.2902143353789413No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT3780.2856797363886454No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG3590.2713201729193748No Hit
CTACCGATTGAGCAATCCGGTGAATATTTCGGACCGGACCCTGTCGGGGC3490.26376250793554823No Hit
CTACCGATTGAGTGTTCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT3100.2342876144986245No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT3060.23126454850509384No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG2940.22219535052450196No Hit
CTACCGATTGGATGGTCCGGTGAGGAGTCGAGATTGTGGCCTGGTTCCTT2890.21841651803258866No Hit
TCCTCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA2890.21841651803258866No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC2890.21841651803258866No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC2780.2101030865503794No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA2730.2063242540584661No Hit
TCCCCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA2660.2010338885697875No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG2640.19952235557302217No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2610.1972550560778742No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG2600.19649928957949153No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2500.18894162459566494No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT2270.17155899513286374No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2180.1647570966474198No Hit
CCCTCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA2170.16400133014903714No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG2160.16324556365065448No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT2140.1617340306538892No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG2100.15871096466035853No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT2100.15871096466035853No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGTGCCTCGA2090.15795519816197587No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCGCGTGGCTTCG2050.15493213216844523No Hit
CCCCCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA2040.15417636567006257No Hit
CTACCGATCGAAGGTAATGATGAGCCCTCAGGACAGTTGATGGAGCCCTC2040.15417636567006257No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG2020.15266483267329725No Hit
CTACCGATTTAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA1950.14737446718461863No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC1920.14510716768947066No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG1880.14208410169594002No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG1780.13452643671211342No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1750.13225913721696544No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1720.12999183772181747No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTTTGGAGGTT1660.1254572387315215No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG1630.12318993923637353No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC1600.12092263974122555No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1540.1163880407509296No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1540.1163880407509296No Hit
TCCTCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTAGATTGTG1520.11487650775416428No Hit
CTACCGATTGAACGGCTTAGTGAGTTTCGAGGATTTGGGTACTGTTCGGT1490.1126092082590163No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCCTCG1420.10731884277033767No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCGT1370.10354001027842437No Hit
TCCCCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTAGATTGTG1350.10202847728165906No Hit
CTACCGATTGAGTGATCCTGTGAATAATTCGGACCGCAGCAATGTTTGGA1340.10127271078327639No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)