FastQCFastQC Report
Tue 22 Apr 2025
Site5r_S123_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5r_S123_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195731
Total Bases33.3 Mbp
Sequences flagged as poor quality0
Sequence length43-281
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG109425.590325497749462No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT98195.0165788761105805No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT84474.315616841481421No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT83814.281897093459901No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT76113.888500033208843No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT58322.9795995524469805No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAGCTCCGGAGGTT57922.9591633415248477No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT40962.092667998426412No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG35151.795832034782431No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG34951.7856139293213644No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT34631.7692649605836583No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT26921.3753569950595461No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG24161.2343471396968289No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG24141.2333253291507222No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT22861.1679294541998968No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG19891.0161905881030597No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG18120.9257603547726216No Hit
CTACTGATTGGGTGTATGAGCGAGGTTCGCGGACGCTTATGATGGGTAAT17980.9186076809498751No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG17950.9170749651307151No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG16980.8675171536445427No Hit
CTACCGATTGAACGATTCGGTGAAATCTCGGGACCATGGTTGTCGCCTCA16750.8557663323643163No Hit
CTACTGATTGAATACATCGGTGAAATATTGGGAATGTGATTTTTAAAGCT16370.8363519319882902No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG15230.7781087308602113No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTTAGT14580.7448998881117451No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCGCGTGGCTTCG14060.7183328139129724No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT14050.717821908639919No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG12850.6565132758735203No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGCCTTGCCTTG12730.6503824125968805No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA11400.5824320112807885No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT11280.5763011480041486No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT10150.5185688521491231No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA10110.5165252310569097No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC9630.4920017779503502No Hit
CTACCGATTGAATGGCTTAGTGAGCTCGAGGGACTGTTGCCGCTTGAGGG9590.48995815685813693No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG9570.4889363463120303No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT9260.4730982828473773No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT8950.45726021938272426No Hit
CTACCGATTGAATGATCCGGTGAGGCCCCCGGACTGGGGCGACGCATTCG8620.4404003453719646No Hit
CTACCGATTGAGTGATCCGGTGAATTGTTCGGACTGCGGCCATTTTCGGT8520.4352912926414314No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTACTTGGTGGGGCT8410.42967133463784485No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG8210.41945322917677835No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG7820.3995279235276987No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG7690.39288615497800555No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGACTGTGGTCTGGCTCCTT7250.37040632296365933No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATTGTGGTCTGTTTCCTT6940.3545682594990063No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG6660.34026291185351326No Hit
CTACCGATTGAATGGTTTAGTGAGACCTTGAGAGGGGGAGCGGGATGGGG6640.3392411013074066No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTTAATGTGGTATTCGAGCC6440.32902299584634015No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6250.319315795658327No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT6180.31573945874695375No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG5960.30449954273978064No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT5870.2999013952823007No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT5830.2978577741900874No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5730.29274872145955416No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5590.2855960476368077No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCTTTGGGACCGCGTATGGCTTGT5420.27691065799490117No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCGCGGCG5210.26618164726078136No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT4510.23041827814704874No Hit
CTACCGATTGAGTGGATTAGTGAGATCCCCGGATCGGCGCCGACATGATG4500.22990737287399543No Hit
CTACCGATTGAACGGTTTAATGAGGTTATTGAATTTGGTTTAGGGGCTTG4330.22122198323208894No Hit
CTACTGATTGGGTGTTCAAGTGAGATCCTTGGACTTTTGATACTTGGTAA4170.21304749886323576No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT4140.21151478304407578No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATCGTGGCGAGTTTCCTT4090.20896025667880921No Hit
CTACCGATTGAATGGCTTAGTGAGATTTCCGGATTGGCGTTAGGAAGCCG3770.19261128794110283No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGATTGGACGTCGAGAGGTT3760.19210038266804952No Hit
CTACCGATTGAATGGTTCGGTGAAATCCTCGGACCGCGCCGCTTCGACCC3720.19005676157583623No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC3580.1829040877530897No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG3560.18188227720698305No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGTCTCGACATGGTG3460.1767732244764498No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG3400.17370779283812987No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGACGGTGCTCG3380.17268598229202325No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT3240.16553330846927672No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT3190.1629787821040101No Hit
CTACCGATTGAATGGCTTAGTGAGCTTTCTGGACTGTTGCGCTTCGTTGG3160.16144606628485011No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAGCTCCGTGGCTT3140.1604242557387435No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT3080.15735882410042354No Hit
CTACCGATTGAATCATTCGGTGAGGATCTCGGATTTTGTTGCTTACGCTT2970.15173886609683698No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGACCGTGACTTTGATTTGT2920.14918433973157036No Hit
CTACCGATTGAATGGTCCGGTGAAGTCTCGAGATTTTGGCTTAGTTGCTT2710.13845532899745058No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA2630.13436808681302398No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTTTGGAGGTT2570.13130265517470405No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT2530.12925903408249076No Hit
CTACCGATTGAGTGGATTAGTGAGATCCCCGGATCGGCGCCGACATGATT2510.12823722353638412No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGATGCATTTCAGTTT2480.12670450771722416No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTTGGACTATGGTTTTCTGATGG2430.12414998135195754No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT2410.12312817080585088No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTATTTGGTGAAGCT2190.11188825479867778No Hit
CTACCGATTGAGTGATTTAGTGAGGTCTTGGGAGGATCTCTGTTGAAGGG2170.11086644425257113No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTGTCTTGGCT2120.1083119178873045No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTTGGGCCGG2050.10473558097593126No Hit
CTACCGATTTCGAGTGATGTGGTGAACATTCTGGACTGCGCTGGCACTAG1970.10064833879150467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)