FastQCFastQC Report
Tue 22 Apr 2025
Site5q_S122_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5q_S122_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157086
Total Bases38.5 Mbp
Sequences flagged as poor quality0
Sequence length24-281
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT22051.4036896986364158No Hit
GAATCAAAATAAATGTTGGTATAAAACAGGGTCGCCTCCCCCCGCAGCGT15070.959347109226793No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT13800.8784996753370765No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT13380.8517627286963829No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT10370.6601479444380786No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT9860.6276816520886648No Hit
AAATCAAAATAAATGTTGGTATAAAACAGGGTCGCCTCCCCCCGCAGCGT9670.6155863667035891No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT9120.5805736984836332No Hit
GAACCAAAATAAATGTTGGTATAAAACAGGGTCGCCTCCCCCCGCAGCGT8710.5544733458105751No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT7440.4736259119208586No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT7280.46344040843868967No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT7280.46344040843868967No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT7170.4564378747946985No Hit
GAATCAAAATAGGTGTTGGTAAAGAATAGGGTCTCCTCCTCCTGCAGGAT6480.4125128910278446No Hit
GAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT6440.40996651515730237No Hit
AAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT6060.385775944387151No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT5820.37049768916389747No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT5650.3596755917140929No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT5650.3596755917140929No Hit
GAACCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT5500.35012668219955945No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT5400.3437607425232038No Hit
GAATCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT5230.33293864507339926No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT5100.324662923494137No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5080.3233897355588658No Hit
AAACCAAAATAAATGTTGGTATAAAACAGGGTCGCCTCCCCCCGCAGCGT4960.3157506079472391No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4450.2832843155978254No Hit
GAATCAAAATAAATGTTGATAAAGTATAGGATCACCCCCACCTTCGTAAT4430.28201112766255426No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT4180.2660962784716652No Hit
GAATCAAAATAAGTGTTGATACAGAACTGGGTCGCCCCCACCTGCCGGAT4140.26354990260112293No Hit
AAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT4120.26227671466585184No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT4050.2578205568924029No Hit
AAATCAAAATAGGTGTTGGTAAAGAATAGGGTCTCCTCCTCCTGCAGGAT4000.25463758705422507No Hit
GAATCAAAATAAATGTTGGTATAAAACGGGATCTCCCCCTCCACTAGCGT3980.253364399118954No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3970.2527278051513184No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3940.2508180232484117No Hit
GAACCAAAATAGGTGTTGGTAAAGAATAGGGTCTCCTCCTCCTGCAGGAT3940.2508180232484117No Hit
GAACCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT3890.24763505341023387No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3810.2425423016691494No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT3590.228537234381167No Hit
GAATCAGAAAAGGTGCTGGTATAGAACAGGGTCACCACCACCAGCTGGAT3510.2234444826400825No Hit
GAATCAAAATAAGTGCTGATAAAGAACAGGATTTCCCCCTCCAGCCGGAT3410.21707854296372686No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3380.21516876106082017No Hit
AAATCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT3360.21389557312554908No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3350.2132589791579135No Hit
GAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT3270.20816622741682897No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT3200.20371006964338004No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3150.20052709980520225No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3060.19479775409648217No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3060.19479775409648217No Hit
GAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT3050.19416116012884663No Hit
GAACCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT3040.19352456616121105No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2850.18142928077613535No Hit
AAACCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT2820.17951949887322868No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA2800.17824631093795754No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2800.17824631093795754No Hit
GAATCAAAATAAATGTTGGTATAAAATTGGATCACCTCCACCTGCGGGAT2500.1591484919088907No Hit
GAACCAAAATAAATGTTGATAAAGTATAGGATCACCCCCACCTTCGTAAT2500.1591484919088907No Hit
AAATCAAAATAAATGTTGATAAAGTATAGGATCACCCCCACCTTCGTAAT2450.15596552207071288No Hit
AAATCAAAATAAGTGTTGATACAGAACTGGGTCGCCCCCACCTGCCGGAT2450.15596552207071288No Hit
AAATCAAAATAAATGTTGGTATAAAACGGGATCTCCCCCTCCACTAGCGT2440.1553289281030773No Hit
GAACCAAAATAAATGTTGGTATAAAACGGGATCTCCCCCTCCACTAGCGT2400.15278255223253506No Hit
GAATCAAAATAAGTGTTGATACAGAACTGGGTCACCCCCACCTGCCGGAT2390.1521459582648995No Hit
GAATCAGAATAGGTGTTGAAATAAAACGGGATCCCCGCCCCCTTCAACAT2330.1483263944590861No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2320.14768980049145053No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2300.1464166125561794No Hit
GAATCAAAATAAATGTTGGTACAAAATAGGGTCTCCTCCTCCTGAAACGT2300.1464166125561794No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2260.14387023668563717No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2250.1432336427180016No Hit
AAACCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT2250.1432336427180016No Hit
AAACCAAAATAGGTGTTGGTAAAGAATAGGGTCTCCTCCTCCTGCAGGAT2200.14005067287982378No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2180.13877748494455266No Hit
GAACCAAAATAAGTGTTGATACAGAACTGGGTCGCCCCCACCTGCCGGAT2160.13750429700928155No Hit
GAATCAATAAGAATTGATAATCTTGCAACATGGGTATCTACAACAACTCC2140.13623110907401043No Hit
AAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT2090.1330481392358326No Hit
AAATCAGAAAAGGTGCTGGTATAGAACAGGGTCACCACCACCAGCTGGAT2070.13177495130056147No Hit
GAATCAAAATAAATGTTGATATAAAATTGGATCACCACCTCCTGCTGGAT2040.1298651693976548No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG2020.12859198146238365No Hit
GAACCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT2010.12795538749474808No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT1990.126682199559477No Hit
GAACCAAAATAAGTGCTGATAAAGAACAGGATTTCCCCCTCCAGCCGGAT1960.12477241765657028No Hit
GAATCAAAATAAATGTTGATATAATATTGGATCTCCTCCGCCGGTAGGAT1950.12413582368893472No Hit
AAATCAAAATAAGTGCTGATAAAGAACAGGATTTCCCCCTCCAGCCGGAT1950.12413582368893472No Hit
GAACCAGAAAAGGTGCTGGTATAGAACAGGGTCACCACCACCAGCTGGAT1940.12349922972129918No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT1910.12158944781839247No Hit
GAATCAAAATAAATGTTGAAACAGAACAGGATCTCCTCCTCCAGCAGGAT1900.12095285385075691No Hit
GAATCAAAATAAGTGTTCAAATAATACTGGTGATCCTCCTTGATAATGTA1880.11967966591548579No Hit
GAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT1850.1177698840125791No Hit
GAATCAAAATAAATGTTGATATAAAATTGGATCACCACCACCTGCTGGAT1840.11713329004494352No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1800.11458691417440128No Hit
GAATCAAAATAGATGTTGATAAAGTATAGGATCACCTCCACCTTCATAAT1780.11331372623913016No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1780.11331372623913016No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1780.11331372623913016No Hit
AAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT1770.11267713227149459No Hit
GAATCAGAAAAGGTGCTGAAACAGTACTGGGTCACCACCACCAGCCGGGT1730.11013075640095235No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1730.11013075640095235No Hit
GAATCAAAATAAATGTTGATATAATATTGGATCACCTCCACCTGCTGGAT1710.10885756846568122No Hit
GAACCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT1690.10758438053041008No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT1670.10631119259513896No Hit
GAATCAAAATAAATGTTGATAAAGTATAGGATCACCTCCACCTTCATAAT1660.1056745986275034No Hit
AAATCAAAATAAATGTTGGTACAAAATAGGGTCTCCTCCTCCTGAAACGT1650.10503800465986786No Hit
AAACCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT1640.10440141069223227No Hit
GAATCAAAATAAATGTTGATATAAAACAGGGTCACCTCCACCAGCAGGAT1610.10249162878932559No Hit
AAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1590.10121844085405447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)