FastQCFastQC Report
Tue 22 Apr 2025
Site5q_S122_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5q_S122_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157086
Total Bases38.5 Mbp
Sequences flagged as poor quality0
Sequence length32-281
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG17051.0853927148186344No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG15971.0166405663139937No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG14510.9236978470392015No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG14410.9173319073628459No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG12590.8014718052531735No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG12110.7709152948066664No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG11510.7327196567485327No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG11470.7301732808779904No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG8170.5200972715582547No Hit
TCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG7960.506728798237908No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG7650.4869943852412055No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG7350.4678965662121386No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG6840.43543027386272487No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG6490.4131494849954802No Hit
TCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG6480.4125128910278446No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG6390.40678354531912453No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG6360.4048737634162179No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG6130.39023210216059995No Hit
CCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG5990.381319786613702No Hit
CCCCCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTGCT5890.37495384693734646No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG5840.3717708770991686No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG5680.3615853736169996No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG5630.35840240377882177No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG5380.34248755458793273No Hit
TCCTCCTTTGTCCGCTATTCACGGGAGCTCCGTTGATTTGGCTATTTTTT5200.3310288631704926No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG5190.330392269202857No Hit
TCCCCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTGCT5180.3297556752352215No Hit
CCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG5080.3233897355588658No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG5070.3227531415912303No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG5020.31957017175305247No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG5010.3189335777854169No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4910.31256763810906124No Hit
TCCTCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTGCT4880.3106578562061546No Hit
TCCCCCTTTGTCCGCTATTCACGGGAGCTCCGTTGATTTGGCTATTTTTT4740.3017455406592567No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4680.29792597685344335No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG4590.2921966311447233No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG4580.2915600371770877No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG4470.2845575035330965No Hit
CCCTCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTGCT4370.2781915638567409No Hit
TCCTCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA4190.26673287243930077No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG4170.2654596845040296No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4090.26036693276294515No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3910.24890824134550502No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3900.24827164737786944No Hit
CCCCCCTTTGTCCGCTATTCACGGGAGCTCCGTTGATTTGGCTATTTTTT3880.24699845944259835No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3790.24126911373387824No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3680.23426658008988704No Hit
TCCTCCCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTG3670.23362998612225153No Hit
CCCTCCTTTGTCCGCTATTCACGGGAGCTCCGTTGATTTGGCTATTTTTT3640.23172020421934483No Hit
TCCTCCGTTATCGGGGCCTATAGCACATTCAGGTGCTTCAGTAGATTTGG3560.22662745247826033No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3540.22535426454298918No Hit
TCCCCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA3440.21898832486663355No Hit
TCCTCCGAGCCTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATAC3110.19798072393465999No Hit
CCCCCCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTGC3040.19352456616121105No Hit
TCCTCCGAGCCTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATAC3020.19225137822593993No Hit
CCCTCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA2990.19034159632303324No Hit
TCCTCCGAGCCTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATAC2970.18906840838776212No Hit
CCCCCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA2970.18906840838776212No Hit
TCCTCCGCTGGCCAGAAATATTGCTCACGCTGGACCTTCAGTAGATCTAG2940.18715862648485543No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2910.18524884458194874No Hit
TCCCCCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTGC2880.18333906267904207No Hit
TCCTCCTTTATCAGGTATTATTGCACACTCTGGTGGTGCTGTTGATTTAG2810.1788829049055931No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2800.17824631093795754No Hit
TCCTCCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTGC2770.17633652903505087No Hit
TCCCCCCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTG2770.17633652903505087No Hit
TCCTCCGAGCCTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATAC2710.17251696522923748No Hit
TCCTCCATTATCAAGTATAGAAGCTCACTCGGGTGCTTCTGTTGATTTAG2710.17251696522923748No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG2710.17251696522923748No Hit
TCCCCCGTTATCGGGGCCTATAGCACATTCAGGTGCTTCAGTAGATTTGG2700.1718803712616019No Hit
TCCTCCTTTATCTGATTTAAAGTTTCATAGAGGAATTTCAGTTGACATGG2650.1686974014234241No Hit
TCCCCCGCTGGCCAGAAATATTGCTCACGCTGGACCTTCAGTAGATCTAG2570.16360464968233962No Hit
TCCCCCATTATCAAGTATAGAAGCTCACTCGGGTGCTTCTGTTGATTTAG2530.16105827381179735No Hit
TCCCCCTTTATCAGGTATTATTGCACACTCTGGTGGTGCTGTTGATTTAG2530.16105827381179735No Hit
CCCTCCGTTATCGGGGCCTATAGCACATTCAGGTGCTTCAGTAGATTTGG2500.1591484919088907No Hit
CCCTCCCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTG2480.15787530397361954No Hit
TCCCCCGAGCCTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATAC2400.15278255223253506No Hit
TCCCCCTTTATCTGATTTAAAGTTTCATAGAGGAATTTCAGTTGACATGG2350.14959958239435725No Hit
CCCTCCCTGAGAAGCAATATTGCACATGCAGGAGCCTCTGTGGACTTTGC2310.14705320652381498No Hit
CCCTCCATTATCAAGTATAGAAGCTCACTCGGGTGCTTCTGTTGATTTAG2280.1451434246209083No Hit
TCCTCCGAGCCTGATTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATAC2250.1432336427180016No Hit
CCCCCCGTTATCGGGGCCTATAGCACATTCAGGTGCTTCAGTAGATTTGG2190.13941407891218824No Hit
CCCCCCATTATCAAGTATAGAAGCTCACTCGGGTGCTTCTGTTGATTTAG2140.13623110907401043No Hit
CCCTCCGAGCCTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATAC2110.13432132717110373No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT2090.1330481392358326No Hit
TCCTCCTTTATCTGGCATTACAGGGCACTCCGGATCCAGCATAGATTTAG2090.1330481392358326No Hit
CCCCCCGCTGGCCAGAAATATTGCTCACGCTGGACCTTCAGTAGATCTAG2080.13241154526819704No Hit
CCCTCCGAGCCTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATAC2080.13241154526819704No Hit
TCCCCCGAGCCTGATTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATAC2070.13177495130056147No Hit
CCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2060.13113835733292592No Hit
CCCCCCTTTATCAGGTATTATTGCACACTCTGGTGGTGCTGTTGATTTAG2050.13050176336529035No Hit
TCCTCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG2010.12795538749474808No Hit
TCCTCCGAGCCTGATTCTTTGGCCACCCCGAGGTCTACTGTCTCTTATAC2000.12731879352711253No Hit
CCCTCCGCTGGCCAGAAATATTGCTCACGCTGGACCTTCAGTAGATCTAG2000.12731879352711253No Hit
TCCCCCGAGCCTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATAC1930.1228626357536636No Hit
CCCCCCTTTATCTGATTTAAAGTTTCATAGAGGAATTTCAGTTGACATGG1890.12031625988312133No Hit
TCCTCCGCTATCTGCTTGATTAGGACACCCTGCTCCGGCAATAGAATTTA1880.11967966591548579No Hit
TCCCCCTTTATCTGGCATTACAGGGCACTCCGGATCCAGCATAGATTTAG1870.11904307194785023No Hit
CCCTCCGAGCCTGATTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATAC1860.11840647798021466No Hit
TCCTCCTTTATCTTCAATTGAAGCACATTCAGGAGGATCTGTTGATTTAG1840.11713329004494352No Hit
TCCCCCGAGCCTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATAC1840.11713329004494352No Hit
TCCTCCGAGCCTGATTCTTCGGCCATCCCGAGGTCTACTGTCTCTTATAC1830.11649669607730798No Hit
TCCCCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG1820.11586010210967242No Hit
TCCCCCTTTATCTTCAATTGAAGCACATTCAGGAGGATCTGTTGATTTAG1800.11458691417440128No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGATTTTG1790.11395032020676571No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGATTTTG1770.11267713227149459No Hit
TCCTCCGAGCCTGATTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATAC1760.11204053830385903No Hit
CCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG1750.11140394433622347No Hit
CCCTCCGAGCCTGATTCTTCGGCCACCCCGAGGTCTACTGTCTCTTATAC1750.11140394433622347No Hit
CCCTCCTTTATCAGGTATTATTGCACACTCTGGTGGTGCTGTTGATTTAG1740.1107673503685879No Hit
CCCCCCTTTATCTGGCATTACAGGGCACTCCGGATCCAGCATAGATTTAG1700.10822097449804567No Hit
TCCTCCGAGCCTGGTTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATAC1700.10822097449804567No Hit
TCCCCCGAGCCTGATTTTTCGGCCACCCCGAGGTCTACTGTCTCTTATAC1670.10631119259513896No Hit
CCCTCCTTTATCTGATTTAAAGTTTCATAGAGGAATTTCAGTTGACATGG1650.10503800465986786No Hit
TCCTCCGAGCCTGATTTTTTGGCCACCCCGAAGTCTACTGTCTCTTATAC1630.10376481672459673No Hit
TCCTCCGTTGGCCAGAAATATTGCTCACGCTGGACCCTCAGTAGATCTAG1630.10376481672459673No Hit
CCCTCCGAGCCTGATTCTTCGGCCATCCCGAAGTCTACTGTCTCTTATAC1630.10376481672459673No Hit
TCCCCCGCTATCTGCTTGATTAGGACACCCTGCTCCGGCAATAGAATTTA1620.10312822275696115No Hit
CCCCCCGAGCCTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATAC1620.10312822275696115No Hit
TCCTCCGAGCCTGATTTTTCGGCCATCCCGAAGTCTACTGTCTCTTATAC1620.10312822275696115No Hit
TCCTCCGAGCCTGGTTCTTTGGCCACCCCGAAGTCTACTGTCTCTTATAC1600.10185503482169002No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG1590.10121844085405447No Hit
TCCTCCGAGCCTGGTTTTTCGGCCACCCCGAAGTCTACTGTCTCTTATAC1580.10058184688641891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)