FastQCFastQC Report
Tue 22 Apr 2025
Site5p_S121_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5p_S121_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149569
Total Bases27 Mbp
Sequences flagged as poor quality0
Sequence length58-281
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG2007713.42323609838937No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG135629.067386958527504No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG104106.959998395389419No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG80945.4115491846572485No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC73704.927491659367917No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT40802.727837987818331No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA28201.8854174327567876No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG27061.8091984301559814No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA24921.6661206533439414No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG23591.5771984836430009No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA11640.7782361318187593No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT10820.7234119369655477No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC10400.6953312517968295No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG9300.6217866001644726No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG9060.6057404943537765No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT8740.5843456866061817No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG7640.5108010349738248No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT7590.5074580962632631No Hit
GAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT7440.4974292801315781No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6280.4198731020465471No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG6220.4158615755938731No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG5880.39312959236205364No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT5660.3784206620355822No Hit
AAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT5650.3777520742934699No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG5220.34900280138263945No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA5160.34499127492996545No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA5070.3389739852509544No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG5030.33629963428250503No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC4810.3215907039560337No Hit
GAACCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT4400.29417860652942784No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA4270.28548696588196754No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT4240.28348120265563054No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG3800.2540633420026877No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT3760.2513889910342384No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC3750.2507204032921261No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA3260.21795960392862157No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3260.21795960392862157No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2970.19857055940736384No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGATAAGGTTTAC2950.19723338392313916No Hit
GAATCAAAAAAGATGCTGGTATAAAATTGGATCTCCCCCCCCTCTTGCAT2900.19389044521257748No Hit
AAACCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT2870.19188468198624045No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2860.19121609424412814No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2610.17450140069131972No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA2600.17383281294920738No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTCGG2530.16915269875442104No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA2520.1684841110123087No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA2490.16647834778597168No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA2280.15243800520161263No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA2230.14909506649105095No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT2210.14775789100682626No Hit
AAATCAAAAAAGATGCTGGTATAAAATTGGATCTCCCCCCCCTCTTGCAT2190.1464207155226016No Hit
GAATCAAAATAGATGCTGGTATAAAACAGGATCTCCGCCTCCTCTGGGGT2170.14508354003837695No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA2120.14174060132781527No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2020.1350547239066919No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT1940.1297060219697932No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1920.12836884648556854No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTAA1920.12836884648556854No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGT1870.12502590777500686No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA1860.12435732003289453No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT1830.12235155680655752No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT1770.11834003035388349No Hit
GAATCAAAATAAGTGTTGATACAGAACTGGGTCACCCCCACCTGCCGGAT1680.11232274067487247No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTTGT1680.11232274067487247No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1650.11031697744853546No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT1640.10964838970642311No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGA1610.1076426264800861No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG1610.1076426264800861No Hit
AAATCAAAATAGATGCTGGTATAAAACAGGATCTCCGCCTCCTCTGGGGT1600.10697403873797377No Hit
AAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT1590.10630545099586146No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC1570.10496827551163677No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT1510.10095674905896276No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC1500.10028816131685042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)