FastQCFastQC Report
Tue 22 Apr 2025
Site5p_S121_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5p_S121_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149569
Total Bases27 Mbp
Sequences flagged as poor quality0
Sequence length58-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT2094214.001564495316543No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT131148.767859650061176No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT84885.674972755049509No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT54413.6377859048332204No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA42742.8575440097881244No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATCGGCGCTCTGGAGAAT36222.4216248019308813No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT33262.22372283026563No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG24461.6353656172067739No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT23041.5404261578268224No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG12320.8237000982823981No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG11430.7641957892344002No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTTCGGAGGCT10960.7327721653551205No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGATGCATTTCAGTTT9930.6639076279175498No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG9920.6632390401754373No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG9890.6612332769491004No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT9280.6204494246802479No Hit
CTAGCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT9090.6077462575801136No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG8600.5749854582166091No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG8030.5368759569162059No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG7660.5121382104580494No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG7390.4940863414210164No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT7160.4787088233524327No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT6930.46333130528384897No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACGTCGAGAGGTT6870.4593197788311749No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG6660.4452794362468158No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6550.4379249710835802No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG6470.43257626914668146No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6400.4278961549518951No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG6380.4265589794676704No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA5780.38644371494093027No Hit
CTAGCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT5360.3583630297722122No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT5260.3516771523510888No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT5110.34164833621940377No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG5070.3389739852509544No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG4900.3276079936350447No Hit
TCCTCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG4660.3115618878243486No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG4570.3055445981453376No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG4370.29217284330309085No Hit
TCCCCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG4320.2888299045925292No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG4290.2868241413661922No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG4130.2761267374923948No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTGTCTTGGCT3930.2627549826501481No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT3750.2507204032921261No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG3650.2440345258710027No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT3560.23801723619199167No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT3460.2313313587708683No Hit
CCCCCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG3450.23066277102875596No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC3400.22731983231819428No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT3360.22464548134974494No Hit
CCCTCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG3120.2085993755390489No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGTT2950.19723338392313916No Hit
CTACCGATCGGATGGTTTAGTGAGACCCTCGGACTGGCCCCGTCGAGGCA2610.17450140069131972No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCGCTCCGGAGGTT2610.17450140069131972No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCTGGCATGGCG2570.17182704972287038No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT2550.1704898742386457No Hit
CTACCGATTGAATGGTTTAGTGAGATGTTCGGATTGGCGCCACTGCTCTT2500.16714693552808402No Hit
CTACCGATTGAGTGGTCCGGTGAACCCTCGGGATCGCATGCAATTTCTTT2390.15979247036484834No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAATGTTTGGA2360.15778670713851134No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT2340.15644953165428666No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2320.15511235617006197No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGCT2290.15310659294372497No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGACCCGTTTTGGAG2290.15310659294372497No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2280.15243800520161263No Hit
CTACCGATTGGCTGTGGGATAGAGCTGGACGGAGATGCTCGTTGGGGGCT2270.1517694174595003No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2110.14107201358570293No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1990.13304896068035488No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1930.12903743422768085No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1910.1277002587434562No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTG1900.12703167100134385No Hit
TCCTCCTTTAAGATCTAATATTTCTCACTCCGGGGCGTCAGTAGATTTTG1860.12435732003289453No Hit
CTACCGATTGAACGGCTTAGTGAAATGTGAGGACCGAGGGGTGTGCAGGG1850.12368873229078219No Hit
CTACCGATTGGATGGTCCGGTGAGAATTTCGGATTGTGGTTGATTTGAGT1830.12235155680655752No Hit
TCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG1810.12101438132233285No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC1670.11165415293276013No Hit
CTAGCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT1600.10697403873797377No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCTGTAGTGCCCAGT1590.10630545099586146No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1590.10630545099586146No Hit
TCCCCCTTTAAGATCTAATATTTCTCACTCCGGGGCGTCAGTAGATTTTG1550.1036311000274121No Hit
CTACCGATTGAATGGCTTAGTGAGCTGCACGGAGTGCGGCACCCGGTTGG1550.1036311000274121No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1540.10296251228529976No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT1510.10095674905896276No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC1500.10028816131685042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)