FastQCFastQC Report
Tue 22 Apr 2025
Site5o_S116_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5o_S116_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109622
Total Bases20.7 Mbp
Sequences flagged as poor quality0
Sequence length56-281
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG1717015.662914378500666No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA69746.361861670102717No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA58795.362974585393443No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG47004.287460546240718No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG28802.6272098666326102No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA21051.9202349893269597No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC19751.8016456550692381No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG19281.7587710496068307No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCTACAGTTCGG15281.3938807903523016No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG14001.2771159073908522No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT13551.2360657532247177No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG12611.1503165422999033No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT11171.018956048968273No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT10320.9414168688766853No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT10260.9359435149878674No Hit
CTACGGAAACCTTGTTACGACTTCAGCTTCTTCTAAATAAATAGGTTTAA9340.8520187553593257No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT8640.788162959989783No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA8610.7854262830453741No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT7820.7133604568426046No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT7530.6869059130466512No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT7450.6796081078615606No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7170.6540657897137435No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6800.6203134407326997No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG6500.59294667128861No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6410.584736640455383No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6190.5646676761963839No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC6100.556457645363157No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT5810.5300031015672036No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT5680.5181441681414315No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG5590.5099341373082046No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT5500.5017241064749777No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5190.47344511138275164No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4850.44242943934611667No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4800.437868311105435No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4750.43330718286475345No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4650.42418492638339017No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA4640.4232727007352539No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT4280.3904325774023462No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4040.36853916184707447No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3990.3639780336063929No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG3890.35485577712502964No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT3820.3484701975880754No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA3560.324752330736531No Hit
AAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3530.32201565379212205No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG3360.30650781777380454No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3340.30468336647753186No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTCGG3310.3019466895331229No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3240.29556110999616864No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3200.29191220740362334No Hit
AAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3070.2800532739778512No Hit
AAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3060.2791410483297148No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2960.2700187918483516No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2850.25998430971885206No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA2450.22349528379339914No Hit
GAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2440.2225830581452628No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG2260.20616299647880898No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA2220.20251409388626368No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA2130.1943040630530368No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2100.19156738610862784No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA2090.1906551604604915No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT2060.18791848351608254No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAA2000.18244512962726459No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA1990.18153290397912827No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC1940.17697177573844666No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1920.175147324442174No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG1730.15781503712758388No Hit
CTACGATAACCTTGTTACGACTTTTTCTTCCTCCAATACAAAGCATTCGC1730.15781503712758388No Hit
AAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1710.15599058583131123No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT1640.14960500629435697No Hit
GAATCAAAATAGGTGCTGGTACAACACAGGGTCTCCCCCGCCGGCTACAT1620.14778055499808432No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC1610.146868329349948No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGAAATTCAGTTTGA1530.1395705241648574No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT1420.12953604203535787No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA1380.12588713944281257No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA1350.12315046249840361No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA1340.12223823685026727No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGA1340.12223823685026727No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT1340.12223823685026727No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG1290.11767710860958566No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT1250.11402820601704036No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA1220.1112915290726314No Hit
AAATCAAAATAGGTGCTGGTACAACACAGGGTCTCCCCCGCCGGCTACAT1210.11037930342449509No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTTAA1180.1076426264800861No Hit
GAATCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT1120.10216927259126818No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT1110.10125704694313184No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGATTTAC1110.10125704694313184No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT1100.10034482129499553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)