FastQCFastQC Report
Tue 22 Apr 2025
Site5o_S116_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5o_S116_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109622
Total Bases20.7 Mbp
Sequences flagged as poor quality0
Sequence length56-281
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT96538.805714181459926No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG68956.289795843899946No Hit
CTACCGATTGAATGGTTTAGTGAGGTTATCGGATTCGGGTTTGTGATTTG55985.106639178267136No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT47324.316651766981081No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT44544.063053036799183No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG39163.5722756381018406No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG33383.0450092134790463No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG27472.505883855430479No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG25292.307018664136761No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT24032.192078232471584No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT19321.7624199521993762No Hit
CTACCGATTGAGTGTTTTAGTGAGGTCCTCGGATTGTTTGCCTGGTGGCT15291.3947930160004378No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT12801.1676488296144933No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG11241.025341628505227No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT11201.0216927259126818No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG10520.9596613818394119No Hit
CTACTGATTGAATACATCGGTGAAATATTGGGAATGTGATTTTTAAAGCT9230.841984273229826No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG9220.8410720475816899No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT8450.7708306726751929No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACGTCGAGAGGTT8300.7571472879531481No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG8150.7434639032311032No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG7530.6869059130466512No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG7490.6832570104541059No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTACTTGGTGGGGCT6280.5728777070296108No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6260.5710532557333382No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6030.5500720658262028No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA5860.5345642298078853No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT5600.5108463629563409No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG5390.49168962434547814No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA4990.4552005984200252No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATTGTGGTCTGTTTCCTT3600.3284012333290763No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT3530.32201565379212205No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG3330.30377114082939555No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATCGTGGCGAGTTTCCTT3290.30012223823685025No Hit
CTACCGATTGAATGGCTTAGTGAGATCCTCGGATGGGTCCCGGCGTGCCC3040.2773165970334422No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTGTCTTGGCT2970.27093101749648796No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG2860.26089653536698837No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT2800.25542318147817045No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG2610.23809089416358029No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2600.237178668515444No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTCCGAAGGTT2190.19977741694185472No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGCGACGCCAGAGCGC2190.19977741694185472No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG2190.19977741694185472No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT2180.19886519129371844No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT2180.19886519129371844No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT2170.1979529656455821No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2070.18883070916421885No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2040.18609403221980989No Hit
CTACCGATTGAATCATTCGGTGAGGATCTCGGATTTTGTTGCTTACGCTT1980.18062067833099196No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT1900.17332287314590136No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG1800.16420061666453814No Hit
CTACCGATTGAGTGATTTAGTGAGGTCTTGGGAGGATCTCTGTTGAAGGG1710.15599058583131123No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGACCGTGACTTTGATTTGT1710.15599058583131123No Hit
CTACCGATTGAATGGCTTAGTGAGCTTTCTGGACTGTTGCGCTTCGTTGG1630.14869278064622066No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGACCCGTTTTGGAG1590.14504387805367536No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT1530.1395705241648574No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCTGGTTTCCTT1520.1386582985167211No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT1490.13592162157231213No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGTTTAGTTCCCTT1370.12497491379467626No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT1330.12132601120213096No Hit
CTACCGATTGAATGGCTTAGGGAGCTCTCTGGACTGTGTTGCGTGTCGTA1270.11585265731331301No Hit
TCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG1190.10855485212822244No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGTGGCTTGTGCGCTT1170.10673040083194979No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC1170.10673040083194979No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG1160.10581817518381348No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG1160.10581817518381348No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT1150.10490594953567714No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC1120.10216927259126818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)