FastQCFastQC Report
Tue 22 Apr 2025
Site5n_S115_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5n_S115_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109769
Total Bases19.4 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA70736.443531415973544No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG63195.7566343867576455No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG59785.445982016780694No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC46194.207927556960526No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA43253.9400923758073776No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG35623.244996310433729No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG27742.527125144621888No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC21171.9285955051061776No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG18721.7053995208118868No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG17931.6334302034271972No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA12951.179750202698394No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG11961.08956080496315No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT11141.0148584755258772No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG10290.9374231340360211No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA9300.8472337363007771No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA7300.6650329327952337No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA6850.6240377520064864No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCATCTAAATAATCAAGTTCGG6720.6121946997786261No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT6700.6103726917435706No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTAG6670.6076396796909874No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6410.5839535752352668No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG6390.5821315672002113No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG6130.5584454627444907No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA5500.5010522096402446No Hit
CTACAGATACCTTGTTACGACTTCTCCTTCCTCTAAGTAATAAGGTTCAT5440.4955861855350782No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA4600.41906184806275No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGAGATATGATTTAC4550.4145068279751114No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAG4490.4090408038699451No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC4180.38079967932658587No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT4100.3735116471863641No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT4070.37077863513378095No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT4050.3689566270987255No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG3960.36075759094097604No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT3680.3352494784502No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3650.33251646639761684No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT3610.32887245032750595No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT3410.3106523699769516No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA3340.3042753418542576No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGGTTTGG3260.2969873097140358No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA3250.2960763056965081No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA3070.2796782333810092No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3050.2778562253459538No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC3050.2778562253459538No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT3010.2742122092758429No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATGTGGTTTAC3010.2742122092758429No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA2910.2651021691005657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2890.2632801610655103No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC2830.257814136960344No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC2820.2569031329428163No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2810.25599212892528855No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC2760.25143710883764997No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTCGA2740.24961510080259453No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA2640.24050506062731739No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2580.23503903652215105No Hit
CTACGGAAACATTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT2540.2313950204520402No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT2430.22137397625923533No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG2420.22046297224170758No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG2370.215907952154069No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCACCAGTTCGA2370.215907952154069No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT2280.20770891599631952No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2260.20588690796126413No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA2250.20497590394373638No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2220.20224289189115324No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTAGACAAGTTCGA2210.20133188787362552No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2110.19222184769834835No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTTAATAAATAGATTTAC2070.18857783162823746No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT2060.18766682761070977No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2030.1849338155581266No Hit
CTACGGAAACCTTGTTACGACTTCAGCTTCTTCTAAATAAATAGGTTTAA1990.18128979948801574No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT1970.1794677914529603No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG1870.17035775127768313No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA1840.1676247392251No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1830.16671373520757227No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1820.16580273119004454No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGA1790.1630697191374614No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1790.1630697191374614No Hit
CTACGGAAACCTTGTTACGACTTAACCTTCCTCTAAATGATAAGATTTAG1730.15760369503229507No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC1710.15578168699723965No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA1690.1539596789621842No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1670.1521376709271288No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGATAAGGTTTAC1660.15122666690960107No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1640.14940465887454565No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATAAGGTTTGG1640.14940465887454565No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC1610.1466716468219625No Hit
CTACGAAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGATTCAC1580.14393863476937935No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA1570.14302763075185163No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT1540.14029461869926846No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG1490.13573959861162987No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG1470.13391759057657446No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT1450.132095582541519No Hit
AAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1430.13027357450646357No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1400.12754056245388043No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1380.12571855441882498No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1370.12480755040129726No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGAAATTCAGTTTGA1340.12207453834871412No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT1330.12116353433118639No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1320.12025253031365869No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1320.12025253031365869No Hit
CTACGGCTACCTTGTTACGACTTCACCCCAGTCGCTGATCCTACCGTGGC1320.12025253031365869No Hit
AAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1270.1156975102260201No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGC1250.11387550219096466No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA1240.11296449817343696No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA1230.11205349415590923No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA1160.1056764660332152No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT1150.1047654620156875No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGTAAGGTTTGA1140.10385445799815976No Hit
AAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1120.10203244996310433No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)