FastQCFastQC Report
Tue 22 Apr 2025
Site5n_S115_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5n_S115_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109769
Total Bases19.3 Mbp
Sequences flagged as poor quality0
Sequence length49-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG66916.095527881277957No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT61365.589920651550074No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT37913.453616230447576No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT30362.7658081972141497No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT29502.687461851706766No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG26392.404139602255646No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT18441.6798914083211107No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA14391.3109347812223853No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG14291.301824741047108No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT13511.2307664276799462No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT13041.1879492388561432No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA12741.1606191183303118No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTGTCTTGGCT12411.1305559857518972No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG11951.0886498009456222No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACCGGACGCTGGCTTCGG10440.951088194298937No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT10400.947444178228826No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC9990.9100930135101895No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG9970.9082710054751342No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT9910.9028049813699679No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT9120.8308356639852781No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG8970.8171706037223624No Hit
CTACCGATTGAATGGTCCGATGAAGCCTCGGGATTGTGGTTTGTTTCCTT7560.6887190372509543No Hit
CTACAGATTGAATGATTTAGTGAGGTCCTCGGACTGGTCCCAGCACGGCC7010.6386138162869298No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG6950.6331477921817635No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT6370.5803095591651559No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT6260.570288514972351No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT6130.5584454627444907No Hit
CTACCGATTGAATGATCCGGTAAATGCTGTGGATTAAGAATGTTGATATT5830.5311153422186592No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5420.49376417750002277No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCCTCG5150.4691670690267744No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCGCGTGGCTTCG5120.46643405697419127No Hit
CTACCGATTGAGGGATTCGGTGAACTTTCTGGACCGTTTCGATCTTTAAG4980.4536800007288032No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTTGGACTGTGACTTTGTTTTGT4870.4436589565359983No Hit
CTACCGATTGAATGGCTTAGTGAGATCCTCGGATGGGTCCCGGCGTGCCC4840.44092594448341516No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC4820.4391039364483597No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG4780.4354599203782489No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT4700.42817188823802715No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG4420.40266377574725104No Hit
CTACCGATTGAATGGCTTAGTGAGATTTCCGGATTGGCGTTAGGAAGCCG4330.39446473958950157No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCCGGCATGGTG4270.3889987154843353No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCTCAGCGAGGCGGACG4050.3689566270987255No Hit
CTACCGATTGAATGATCCGGTGAGGCCCCCGGACTGGGGCGACGCATTCG3910.35620257085333745No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG3820.348003534695588No Hit
CTACCGATTGAATGGCTTAGTGAGCTTTCTGGACTGTTGCGCTTCGTTGG3760.3425375105904217No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG3720.3388934945203108No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3630.33069445836256134No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT3630.33069445836256134No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA3380.3079193579243684No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG3380.3079193579243684No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC3330.3033643378367299No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG3320.30245333381920214No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCGGGAGGGT3270.29789831373156356No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT3050.2778562253459538No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2980.27147919722325975No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGACGGTGCTCG2910.2651021691005657No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG2890.2632801610655103No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2870.26145815303045483No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2780.2532591168727054No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG2740.24961510080259453No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCCCGCCC2700.24597108473248366No Hit
CTAGCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT2670.24323807267990052No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGACTGTGGTCTGGCTCCTT2600.2368610445572065No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2480.2259289963468739No Hit
CTACCGATTGGGTGTACCAGTGAGATCTTTGGACTGGCGAGCTCTAGCTC2420.22046297224170758No Hit
CTACCGATTCAATGAATCGATGAACTGGATGGAGTGGCCTTATTAGGGGC2390.21772996018912444No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGACCCGTTTTGGAG2390.21772996018912444No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCACTCCGTTGG2340.21317494010148585No Hit
CCCGAAGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCG2330.21226393608395813No Hit
CTACCGATTGAGTTATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA2310.21044192804890272No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2240.20406489992620866No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG2240.20406489992620866No Hit
CTAGCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT2220.20224289189115324No Hit
CTACTGATTGAATACATCGGTGAAATATTGGGAATGTGATTTTTAAAGCT2210.20133188787362552No Hit
CCCGAGGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCG2200.2004208838560978No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAATGTTTGGA1980.18037879547048802No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAACTCGTGGGCTT1970.1794677914529603No Hit
CTACCGATTTAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT1950.17764578341790488No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG1920.17491277136532174No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTACTTGGTGGGGCT1910.174001767347794No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1870.17035775127768313No Hit
TCCTCCTCTCTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1870.17035775127768313No Hit
CTACCGATTGAGTGGATTAGTGAGATCCCCGGATCGGCGCCGACATGATG1820.16580273119004454No Hit
CTACCGATTGGATGGTCCGGTGAGGAGTCGGGATTGCGGCTTGGTTCCTT1810.16489172717251685No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATGGATGGATTGTG1810.16489172717251685No Hit
CTACCGATTGAGTGCTCAGGTGAATAGTATGGACTGCATCAGTGTTCGGT1760.16033670708487824No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG1760.16033670708487824No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGCGTCTAGT1730.15760369503229507No Hit
CATGGAAGTGGATTGCACCAGAAGTAGATAGTCTAACCTTAGGGAGGGCG1700.15487068297971193No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT1640.14940465887454565No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1630.1484936548570179No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC1630.1484936548570179No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT1570.14302763075185163No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG1570.14302763075185163No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1560.1421166267343239No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATAGATGGACTGTG1500.1366506026291576No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGCGTCTCGT1480.13482859459410215No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATTGTGGTCTGTTTCCTT1470.13391759057657446No Hit
CTACCGATTGAGTGATTTAGTGAGGTCTTGGGAGGATCTCTGTTGAAGGG1460.1330065865590467No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1450.132095582541519No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC1440.1311845785239913No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1430.13027357450646357No Hit
CATGGGAGTTGGTTCTACCCGACGACGCTGCGCTAACCCTTCGGGGAGGC1420.12936257048893585No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1400.12754056245388043No Hit
CTACCGATTGAATGGTTTAGTGAGTCCTCGGGATCAGCCTTTTGAAACGT1400.12754056245388043No Hit
CTACCGATTGAATGATCCGGTGAGCTATTCGGACTTTCGCTTTTTCGGTT1380.12571855441882498No Hit
CCCTCCTCTCTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1340.12207453834871412No Hit
TCCCCCTCTCTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG1340.12207453834871412No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1320.12025253031365869No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG1300.11843052227860325No Hit
CTACCGATTGAACGGCTTAGTGAGTTGTAGGGACCGGAGTCTGCGCGGGG1290.11751951826107553No Hit
CTACCGATTGAGTGGTCCGGTGAACCCTCGGGATCGCATGCAATTTCTTT1270.1156975102260201No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT1250.11387550219096466No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTTCGGAGGCT1250.11387550219096466No Hit
CTACCGATTGAATGGTTTAGTGAGGCCTCCTGATTGGCGCCACCGCCCCG1240.11296449817343696No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG1220.11114249013838153No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG1190.10840947808579836No Hit
CTAGCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT1180.10749847406827064No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1180.10749847406827064No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1140.10385445799815976No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1140.10385445799815976No Hit
CTACCGATTGAATGGTTTAGCGAGATCCTCGGATTGGTCCCGGCATGGTG1120.10203244996310433No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)