FastQCFastQC Report
Tue 22 Apr 2025
Site5m_S110_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5m_S110_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121104
Total Bases22.2 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT1954416.138195270181No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT87767.246664024309685No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT82766.833795745805259No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA44473.672050469018364No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT35862.9610912934337428No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT35502.9313647773814244No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT27682.285638789800502No Hit
CTACCGATTGAGTGTTTTAGTGAGGTCCTCGGATTGTTTGCCTGGTGGCT26532.190679085744484No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG15211.2559453032104637No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG12961.070154577883472No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG12691.047859690844233No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT12361.0206103844629408No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG11540.9528999867882151No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG10320.8521601268331352No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG10140.8372968688069758No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG10030.8282137666798784No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT8810.7274739067247985No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG8440.696921654115471No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG8300.6853613423173471No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG7790.6432487779098957No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT7290.6019619500594531No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG7110.5870986920332938No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT6080.5020478266613819No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG5900.48718456863522264No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT5680.4690183643810279No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG4910.4054366494913463No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC4740.3913991280221958No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG4620.3814902893380896No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG4610.38066455278108075No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT4610.38066455278108075No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4090.3377262518166204No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG3830.3162571013343903No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG3480.28735632183908044No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGATGCATTTCAGTTT3430.2832276390540362No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT3400.28075042938300965No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3290.27166732725591225No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3190.26340996168582376No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG3130.25845554234377066No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT3090.2551525961157352No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG3010.24854670365966441No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG3000.24772096710265556No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2930.24194081120359362No Hit
TCCTCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG2760.22790328973444313No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2740.22625181662042543No Hit
CTACCGATTGAGTGGTCCGGTGAACCCTCGGGATCGCATGCAATTTCTTT2700.22294887039239No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG2700.22294887039239No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2630.21716871449332806No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC2520.2080856123662307No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG2490.20560840269520414No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG2410.19900251023913332No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT2390.1973510371251156No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTGTCTTGGCT2380.19652530056810674No Hit
TCCCCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG2370.1956995640110979No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2160.178359096313912No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG2140.1767076231998943No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT2060.1701017307438235No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA2010.16597304795877924No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC1960.16184436517373496No Hit
CCCCCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG1960.16184436517373496No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG1950.1610186286167261No Hit
CTACCGATTGAGTGATTCGGCGAATGCTTCGGACTGGCTCTTTTAGAGAC1940.16019289205971726No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG1920.15854141894569956No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1880.15523847271766417No Hit
CCCTCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG1810.1494583168186022No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG1770.1461553705905668No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCGT1720.14202668780552252No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGGCCTCGGGAGGTT1710.14120095124851367No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1710.14120095124851367No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG1670.13789800502047828No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGGTTTAGTTCCTT1600.1321178491214163No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG1540.1271634297793632No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT1540.1271634297793632No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG1500.12386048355132778No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG1430.11808032765226584No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCTCGTGGCTTCG1410.11642885453824812No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGCTTGGT1390.11477738142423041No Hit
CTACCGATCGAGTGTTTAGGTGAAATTGATAGACGTAAGGAAGTCAATTA1340.11064869863918615No Hit
CTACCGATTGAATGGCTTAGTGAGCTTTCTGGACTGTTGCGCTTCGTTGG1320.10899722552516844No Hit
CTACCGATTGAGTGATTTAGTGAGGTCTTGGGAGGATCTCTGTTGAAGGG1300.10734575241115075No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT1280.10569427929713304No Hit
CTACCGATTGGCTGTGGGATAGAGCTGGACGGAGATGCTCGTTGGGGGCT1280.10569427929713304No Hit
CTACCGATTGGATGGTCCGGTGAGAATTTCGGATTGTGGTTGATTTGAGT1250.10321706962610648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)