FastQCFastQC Report
Tue 22 Apr 2025
Site5k_S108_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5k_S108_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61179
Total Bases11.5 Mbp
Sequences flagged as poor quality0
Sequence length53-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG806913.18916621716602No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC41796.8307752660226555No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG36195.915428496706386No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG24984.0831004102714985No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG14842.425668938688112No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG11611.897709998528907No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC11421.866653590284248No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA10801.7653116265385183No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC9681.5822422726752643No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG8351.3648474149626506No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA6331.0346687588878536No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA5770.9431340819562268No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG5490.8973667434904133No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC5380.879386717664558No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA4970.8123702577681885No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC3810.6227627126955327No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA3700.6047826868696775No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3500.572091730822668No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3260.5328625835662565No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3230.527958940159205No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3190.521420748949803No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCGC3170.518151653345102No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG3100.5067098187286487No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT3090.5050752709262982No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG3000.49036434070514395No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2880.47074976707693816No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA2770.45276974125108294No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2620.4282515242158257No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA2620.4282515242158257No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2520.41190604619232085No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT2330.3808496379476618No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2280.3726768989359094No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2270.3710423511335589No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA2240.3661387077265074No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT2060.3367168472841989No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT1960.320371369260694No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC1930.3154677258536426No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA1840.30075679563248825No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT1820.2974877000277873No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAA1780.29094950881838544No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGCATCAGGATTTAA1750.286045865411334No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1720.2811422220042825No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG1670.2729694829925301No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT1570.2566240049690253No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT1540.25172036156197386No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA1540.25172036156197386No Hit
AAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT1500.24518217035257198No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT1450.2370094313408196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1410.23047124013141762No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA1400.22883669232906717No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC1400.22883669232906717No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG1360.22229850111966523No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1300.2124912143055624No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGA1300.2124912143055624No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA1290.2108566665032119No Hit
GAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT1270.20758757089851093No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT1260.20595302309616043No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT1160.18960754507265565No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT1150.18797299727030517No Hit
AAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAA1140.1863384494679547No Hit
GAACCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT1100.17980025825855278No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT1100.17980025825855278No Hit
AAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT1090.1781657104562023No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG1060.17326206704915084No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA1000.16345478023504798No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG970.15855113682799654No Hit
GAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT970.15855113682799654No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATATGGTTTAA940.1536474934209451No Hit
AAACCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT930.1520129456185946No Hit
GAACCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAA930.1520129456185946No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC930.1520129456185946No Hit
CTACGAAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGATTCAC920.15037839781624412No Hit
CTACAGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC880.1438402066068422No Hit
AAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT870.14220565880449174No Hit
GAACCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT830.1356674675950898No Hit
GAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT810.13239837199038887No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT800.1307638241880384No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCCCCAACAT770.12586018078098693No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC770.12586018078098693No Hit
CTACGGAAACCTTGTTACGACTTCTGCTTCCTCTAAGTGATAAGGCTTGC750.12259108517628599No Hit
AAACCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAA740.1209565373739355No Hit
AAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT730.11932198957158502No Hit
GAATCAAAATAAATGCTGGTAAAGAATGGGGTCTCCCCCTCCTCTGGCGT730.11932198957158502No Hit
GAATCAAAATAAATGTTGGTATAAAATTGGATCACCTCCACCTGCGGGAT700.11441834616453359No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA680.11114925055983262No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT670.10951470275748214No Hit
GAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT660.10788015495513167No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCCCCAACAA640.10461105935043069No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG640.10461105935043069No Hit
AAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT630.10297651154808021No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG620.10134196374572975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)