FastQCFastQC Report
Tue 22 Apr 2025
Site5k_S108_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5k_S108_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61179
Total Bases11.5 Mbp
Sequences flagged as poor quality0
Sequence length53-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT822013.435982935320945No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT38026.214550744536524No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT26334.303764363588813No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT25294.1337713921443635No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT15912.6005655535396133No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG11381.8601153990748458No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG10701.7489661485150132No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG10451.7081024534562512No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT8511.3910001798002583No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG8401.373020153974403No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG8031.3125418852874353No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG7251.1850471567040979No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG7041.1507216528547377No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT6671.09024338416777No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT6491.0608215237254615No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA6141.0036123506431947No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA4270.6979519116036549No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCCTCG4100.6701645989636967No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT4030.6587227643472433No Hit
CTACCGATTGGATGATCCGGTGAGCTATTCGGACTTTCGCTTTGTCGGTT3850.6293009039049346No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG3470.5671880874156164No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT3460.565553539613266No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT3220.5263243923568545No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGCTAGGACTTG3110.5083443665309992No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG3090.5050752709262982No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3050.4985370797168963No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3050.4985370797168963No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2960.483826149495742No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCGT2840.46421157586753625No Hit
CTACCGATTGAATGGCTTAGTGAGATTTCCGGATTGGCGTTAGGAAGCCG2830.4625770280651858No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2800.45767338465813434No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG2700.44132790663462956No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG2640.4315206198205267No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2640.4315206198205267No Hit
CTACCGATTGAATGGTTTAGTGAGATCTCCGGATTGGCGCCATCGCGGCT2530.41354059399467136No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGTCAGTTTGCTT2530.41354059399467136No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT2490.40700240278526945No Hit
CTACCGATTGAACGTTTTAGTGAGGTACTCGGACTGGACCTCTGGAGATT2490.40700240278526945No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACTGGGCCTTTGGAGGTT2480.405367854982919No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG2390.3906569247617646No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA2350.38411873355236276No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2340.38248418575001225No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG2270.3710423511335589No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC2230.36450415992415697No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2190.35796596871475506No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2180.3563314209124046No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2110.34488958629595123No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2080.3399859428888998No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG2080.3399859428888998No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG2060.3367168472841989No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG2030.3318132038771474No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2020.3301786560747969No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC1980.323640464865395No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG1960.320371369260694No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1960.320371369260694No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1880.3072949868418902No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG1850.30239134343483876No Hit
CTACCGATTGAGCATTAGGGTGAAATCTTCGGACCGTGGCATACTTCTGG1840.30075679563248825No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG1810.2958531522254368No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA1660.2713349351901796No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG1660.2713349351901796No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG1660.2713349351901796No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG1590.2598931005737263No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1580.2582585527713758No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1570.2566240049690253No Hit
CTACCGATTGAACGGCTTAGTGAGTTTTACGGACCGGCGCTGGTGTTGGG1480.241913074747871No Hit
TCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG1430.23374033573611858No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA1280.20922211870086138No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT1240.2026839274914595No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG1230.201049379689109No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT1200.1961457362820576No Hit
CTACCGATTGAGTGCTCAGGTGAATAGTATGGACTGCATCAGTGTTCGGT1160.18960754507265565No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG1150.18797299727030517No Hit
CTACCGATTGAGTGGTTCGGTGAACTTTTTGGACTGCGCAGGCTTTAGTC1140.1863384494679547No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT1110.18143480606090326No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG1080.17653116265385183No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG1030.16835842364209944No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG1020.16672387583974893No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG1010.16508932803739845No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT990.1618202324326975No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG980.160185684630347No Hit
TCCCCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG970.15855113682799654No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG970.15855113682799654No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC930.1520129456185946No Hit
CCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG910.14874385001389365No Hit
CTACCGATTGAGTGATCCGGTGAGTAATTCGGACCAGCTGTCGCCTGAGT900.14710930221154317No Hit
CCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG890.14547475440919272No Hit
TCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG850.13893656319979078No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT800.1307638241880384No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTT790.1291292763856879No Hit
CTACCGATTGAATTGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT790.1291292763856879No Hit
TCCTCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG790.1291292763856879No Hit
CCCCCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG780.1274947285833374No Hit
CCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG730.11932198957158502No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT720.11768744176923454No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG700.11441834616453359No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT700.11441834616453359No Hit
CCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG690.1127837983621831No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT690.1127837983621831No Hit
TCCCCCTTTGTCAGGAATTTTAGCTCATTCTGGTGGCGCTGTCGATTTAG680.11114925055983262No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT680.11114925055983262No Hit
TCCTCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG670.10951470275748214No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC660.10788015495513167No Hit
CCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG650.1062456071527812No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCAGGAATCGCATGAGT620.10134196374572975No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTTGGGAGGAT620.10134196374572975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)