FastQCFastQC Report
Tue 22 Apr 2025
Site5h_S98_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5h_S98_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375556
Total Bases55.3 Mbp
Sequences flagged as poor quality0
Sequence length43-281
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT4231111.266229270734591No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTGTCTTGGCT362659.656349519112995No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG353479.411911938565753No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT222375.921087667351873No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGCCTGGCGGAT190405.069816485424278No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT154874.123752516269211No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT128173.412806612063181No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT112232.9883692445334384No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT100382.6728370735655935No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT92832.4718018085185696No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT85742.2830150496863317No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT75081.9991692317523884No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT71861.9134296882488897No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT68021.8111812885428538No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGTCTCGACATGGTG62871.6740512733121025No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA43451.156951293548765No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACCGGACGCTGGCTTCGG28980.7716558915314894No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG26700.7109459042060305No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTTCGGAGGCT25200.6710051230708604No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACTGGGCCTTTGGAGGTT18210.48488108298096694No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTCCGAAGGTT16230.4321592518825422No Hit
CTACTGATTGGATGATTAAGCAAGAACTCCTGAAACTTGCAGCTCCGGTT14930.3975439082320613No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT14820.3946149176154821No Hit
CTACCGATTGAGTGGTCCGGTGAACCCTCGGGATCGCATGCAATTTCTTT13810.3677214583178008No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG12250.32618304593722375No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT9210.24523639616994536No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTCTGAAGGTT8820.23485179307480109No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT8520.22686363684776703No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT8270.22020683999190532No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT7720.2055618869090096No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATTGTGGTCTGTTTCCTT7140.19011811820341043No Hit
CTACCGATTGAGTGATTTAGTGAGGTCTTGGGAGGATCTCTGTTGAAGGG6830.1818636901021419No Hit
CTACCGATTGAATGGCTTAGTGAGCTTTCTGGACTGTTGCGCTTCGTTGG6680.17786961198862486No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC6660.17733706824015594No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGGCGCCATCGTGGCT6560.17467434949781124No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT6150.16375720265419802No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG6100.1624258432830257No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATCGTGGCGAGTTTCCTT5630.14991106519400568No Hit
CTACTGATTGAATACATCGGTGAAATATTGGGAATGTGATTTTTAAAGCT5620.1496447933197712No Hit
CTACCGATTGGCTGTGGGATAGAGCTGGACGGAGACGCTCGTTGAGGGCT5580.14857970582283334No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT5530.147248346451661No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT5200.13846137460192356No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT4950.13180457774606183No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT4890.13020694650065504No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC4780.12727795588407587No Hit
CTACCGATTGGCTGTGGGATAGAGCTGGACGGAGATGCTCGTTGGGGGCT4650.12381642151902779No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG4480.11928979965704183No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTACTTGGTGGGGCT4360.11609453716622821No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGACCCGTTTTGGAG4350.11582826529199373No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGGTTTAGTTCCTT4180.11130164343000777No Hit
CTACCGATTGAACGGCTTAGTGAGTTGTAGGGACCGGAGTCTGCGCGGGG3800.10118331220909799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)