FastQCFastQC Report
Tue 22 Apr 2025
Site5g_S97_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5g_S97_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106781
Total Bases29.1 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT49954.677798484749159No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT36273.39667169252957No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT26962.5247937367134603No Hit
GAATCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT20541.9235631807156706No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT18721.753120873563649No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT17201.610773452205917No Hit
AAATCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT15691.4693625270413275No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT13691.2820632884127325No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT12901.2080800891544377No Hit
GAACCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT10951.0254633314915576No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT9850.9224487502458303No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT9780.9158932768938295No Hit
GAATCAAAAGATATGTTGGAATAAGATTGGGTCCCCTCCTCCAGCTGGGT9780.9158932768938295No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT9450.8849889025201112No Hit
AAACCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT8110.7594984126389527No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT8010.7501334507075229No Hit
GAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT7640.7154830915612328No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7410.6939436791189444No Hit
GAATCAAAATAGATGCTGGTATAAAACAGGGTCTCCGCCTCCTCTGGGGT7330.6864517095738005No Hit
AAATCAAAAGATATGTTGGAATAAGATTGGGTCCCCTCCTCCAGCTGGGT7260.6798962362217997No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7190.6733407628697989No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT6810.6377539075303659No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6700.6274524494057931No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT6460.6049765407703618No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT6420.6012305559977899No Hit
GAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT6230.5834371283280734No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5930.5553422425337841No Hit
GAACCAAAAGATATGTTGGAATAAGATTGGGTCCCCTCCTCCAGCTGGGT5780.5412947996366395No Hit
AAATCAAAATAGATGCTGGTATAAAACAGGGTCTCCGCCTCCTCTGGGGT5770.5403583034434966No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5540.5188188910012081No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5260.49259699759320474No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5230.4897875090137759No Hit
AAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT5070.47480356992348827No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4970.4654386079920585No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4820.4513911650949139No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT4820.4513911650949139No Hit
GAATCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT4790.4485816765154849No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4700.4401532107771982No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT4600.4307882488457685No Hit
AAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT4500.42142328691433867No Hit
GAACCAAAATAGATGCTGGTATAAAACAGGGTCTCCGCCTCCTCTGGGGT4420.4139313173691949No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4280.4008203706651932No Hit
AAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4220.3952013935063354No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT4190.3923919049269065No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT4160.38958241634747753No Hit
GAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT4010.37553497345033293No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3970.37178898867776106No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3910.36617001151890316No Hit
GAACCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT3780.35399556100804447No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3770.35305906481490157No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3730.34931308004232964No Hit
AAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3580.335265637145185No Hit
AAACCAAAAGATATGTTGGAATAAGATTGGGTCCCCTCCTCCAGCTGGGT3570.334329140952042No Hit
GAACCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT3530.33058315617947015No Hit
AAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3400.31840870566861146No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3290.30810724754403873No Hit
AAATCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT3280.3071707513508958No Hit
GAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT3270.3062342551577528No Hit
GAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT3200.29967878180575197No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3200.29967878180575197No Hit
AAACCAAAATAGATGCTGGTATAAAACAGGGTCTCCGCCTCCTCTGGGGT3170.29686929322632305No Hit
AAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT3170.29686929322632305No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3080.28844082748803623No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2870.2687744074320338No Hit
GAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2860.26783791123889084No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2770.2594094455006041No Hit
AAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT2740.25659995692117515No Hit
AAACCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT2710.25379046834174623No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2670.2500444835691743No Hit
GAATCAAAATAGATGCTGGTATAAAACAGGATCTCCGCCTCCCCTAGGGT2560.23974302544460158No Hit
GAATCAGAATAAGTGTTGGTAGAGCACAGGATCCCCTCCTCCAGAAGGGT2560.23974302544460158No Hit
GAACCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT2550.2388065292514586No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2510.23506054447888666No Hit
GAACCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT2460.2303780635131718No Hit
AAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT2460.2303780635131718No Hit
AAACCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT2430.22756857493374288No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2210.20696565868459746No Hit
AAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT2180.20415617010516854No Hit
GAACCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT2180.20415617010516854No Hit
GAATCAAAATAAGTGTTGATACAGAACTGGGTCACCCCCACCTGCCGGAT2170.20321967391202556No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2150.20134668152573962No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT2030.1901087272080239No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1990.18636274243545198No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT1980.18542624624230902No Hit
AAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1970.18448975004916607No Hit
AAACCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT1940.18168026146973712No Hit
AAATCAGAATAAGTGTTGGTAGAGCACAGGATCCCCTCCTCCAGAAGGGT1930.18074376527659414No Hit
GAACCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT1920.17980726908345118No Hit
AAATCAAAATAAGTGTTGATACAGAACTGGGTCACCCCCACCTGCCGGAT1820.17044230715202144No Hit
AAACCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT1680.1573313604480198No Hit
AAATCAAAATAGATGCTGGTATAAAACAGGATCTCCGCCTCCCCTAGGGT1670.1563948642548768No Hit
GAACCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT1580.14796639851659002No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1540.14422041374401812No Hit
GAACCAAAATAGATGCTGGTATAAAACAGGATCTCCGCCTCCCCTAGGGT1530.14328391755087516No Hit
GAACCAGAATAAGTGTTGGTAGAGCACAGGATCCCCTCCTCCAGAAGGGT1490.13953793277830326No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT1470.13766494039201732No Hit
GAATCAAAATAGATGCTGGTATAAAACAGGGTCTCCGCCTCCCCTAGGGT1440.13485545181258837No Hit
AAACCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT1350.1264269860743016No Hit
GAATCAGAATAAGTGTTGGTAAAGAATGGGGTCACCTCCTCCGGTGGGGT1320.12361749749487268No Hit
GAATCAAAATAAGTGTTGGTATAAGATTGGGTCTCCTCCTCCTGTAGGAT1300.12174450510858673No Hit
AAACCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT1290.12080800891544376No Hit
GAACCAAAATAAGTGTTGATACAGAACTGGGTCACCCCCACCTGCCGGAT1280.11987151272230079No Hit
GAATCAAAATAAATGTTGATAAAGTATAGGATCACCCCCACCTTCGTAAT1280.11987151272230079No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT1210.11331603937029996No Hit
AAACCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT1200.11237954317715698No Hit
AATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGATC1190.11144304698401401No Hit
GAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT1170.10957005459772806No Hit
GAATCAAAATAAATGTTGGTATAAAATTGGATCACCTCCACCTGCGGGAT1170.10957005459772806No Hit
AAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT1160.10863355840458509No Hit
GAATCAGTACCCTGTCTCTTATACACATCTGACGCTGCCGACGATGACCA1130.10582406982515616No Hit
AAACCAAAATAGATGCTGGTATAAAACAGGATCTCCGCCTCCCCTAGGGT1110.1039510774388702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)