FastQCFastQC Report
Tue 22 Apr 2025
Site5g_S97_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5g_S97_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106781
Total Bases29 Mbp
Sequences flagged as poor quality0
Sequence length50-281
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG46784.380929191522837No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG42964.023187645742221No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG40973.836824903306768No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG36413.4097826392335717No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG31692.9677564360700877No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG31222.9237411149923673No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG29672.7785842050552065No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG28262.6465382418220473No Hit
TCCTCCTTTATCCGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG17681.6557252694767797No Hit
TCCCCCTTTATCCGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG15751.4749815042001855No Hit
CCCTCCTTTATCCGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG12281.1500173251795731No Hit
CCCCCCTTTATCCGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG12261.1481443327932872No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG11361.0638596754104195No Hit
TCCTCCGTTGTCGAGAACTCTCGGGCACAATGGTATGAGCTTAGATTTAG9270.8681319710435377No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG9160.857830512918965No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG8610.8063232222961014No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG7260.6798962362217997No Hit
TCCCCCGTTGTCGAGAACTCTCGGGCACAATGGTATGAGCTTAGATTTAG7050.6602298161657973No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG6920.6480553656549386No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG6880.6443093808823668No Hit
TCCTCCTTTAAGATCTAATATTTCTCACTCCGGGGCGTCAGTAGATTTTG6230.5834371283280734No Hit
CCCTCCGTTGTCGAGAACTCTCGGGCACAATGGTATGAGCTTAGATTTAG6130.5740721663966436No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG6120.5731356702035006No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG6020.5637707082720709No Hit
TCCCCCTTTAAGATCTAATATTTCTCACTCCGGGGCGTCAGTAGATTTTG5580.52256487577378No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG5560.5206918833874941No Hit
CCCCCCGTTGTCGAGAACTCTCGGGCACAATGGTATGAGCTTAGATTTAG5550.519755387194351No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG5500.5150729062286361No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4950.4635656156057726No Hit
TCCTCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG4660.43640722600462634No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4630.43359773742519736No Hit
CCCTCCTTTAAGATCTAATATTTCTCACTCCGGGGCGTCAGTAGATTTTG4490.42048679072119566No Hit
CCCCCCTTTAAGATCTAATATTTCTCACTCCGGGGCGTCAGTAGATTTTG4310.4036298592446222No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG4270.39988387447205026No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG4170.39051891254062054No Hit
TCCCCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG4160.38958241634747753No Hit
TCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG3860.3614875305531883No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3800.3558685533943305No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3580.335265637145185No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3530.33058315617947015No Hit
TCCTCCTTTAAGGTCTAATATTTCTCACTCCGGGGCGTCAGTAGATTTTG3440.3221546904411834No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3330.31185323231661066No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG3300.30904374373718174No Hit
CCCCCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG3270.3062342551577528No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG3270.3062342551577528No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3270.3062342551577528No Hit
TCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG3130.2931233084537511No Hit
TCCCCCTTTAAGGTCTAATATTTCTCACTCCGGGGCGTCAGTAGATTTTG3050.2856313389086073No Hit
CCCTCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG3010.28188535413603544No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2970.27813936936346356No Hit
CCCGAAGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCAG2950.27626637697717754No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG2910.2725203922046057No Hit
TCCTCCTTTATCAGGTATTATTGCACACTCTGGTGGTGCTGTTGATTTAG2790.26128243788689No Hit
CCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG2760.25847294930746106No Hit
TCCCCCTTTATCAGGTATTATTGCACACTCTGGTGGTGCTGTTGATTTAG2660.2491079873760313No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG2620.24536200260345942No Hit
CCCGAGGTCTACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCAG2550.2388065292514586No Hit
CCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2520.23599704067202967No Hit
CCCTCCTTTAAGGTCTAATATTTCTCACTCCGGGGCGTCAGTAGATTTTG2410.22569558254745695No Hit
CCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2380.22288609396802803No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2350.2200766053885991No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2250.21071164345716933No Hit
TCCTCCGTTGGCCAGAAATATTGCTCACGCTGGACCTTCAGTAGATCTAG2240.20977514726402638No Hit
CCCCCCTTTAAGGTCTAATATTTCTCACTCCGGGGCGTCAGTAGATTTTG2210.20696565868459746No Hit
CCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG2200.20602916249145448No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1940.18168026146973712No Hit
CCCCCCTTTATCAGGTATTATTGCACACTCTGGTGGTGCTGTTGATTTAG1840.17231529953830738No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1720.1610773452205917No Hit
CCCTCCTTTATCAGGTATTATTGCACACTCTGGTGGTGCTGTTGATTTAG1710.16014084902744868No Hit
CCCTCCGTTGGCCAGAAATATTGCTCACGCTGGACCTTCAGTAGATCTAG1700.15920435283430573No Hit
TCCCCCGTTGGCCAGAAATATTGCTCACGCTGGACCTTCAGTAGATCTAG1510.1414109251645892No Hit
TCCTCCTTTATCTGGCATTACAGGGCACTCCGGATCCAGCATAGATTTAG1410.13204596323315945No Hit
CCCCCCTTAGCTTCAAATATCGCTCATGCTGGACCTTCAGTAGACCTAGC1380.12923647465373053No Hit
TCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG1250.11706202414287187No Hit
TCCTCCATTAGCAAGTATTGCTTTCCATTCAGGAGGAGCGGTTGATTGTG1220.11425253556344293No Hit
TCCTCCCCTATCAGCTGCTACTGCCCACAGAGGGAGATCTGTCGACGTAG1170.10957005459772806No Hit
TCCTCCCTTAGCTTCAAATATCGCTCATGCTGGACCTTCAGTAGACCTAG1170.10957005459772806No Hit
TCCTCCGTTATCGGGGCCTATAGCACATTCAGGTGCTTCAGTAGATTTGG1140.10676056601829913No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG1130.10582406982515616No Hit
TCCTCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG1110.1039510774388702No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG1100.10301458124572724No Hit
TCCTCCTTTAAGCTCTACTCTAGGACATTCGGGGGGATCAGTAGATTTTG1090.10207808505258427No Hit
CCCCCCTTATCGAGAAACATCGCTCACTCAGGGGCTTCTGTGGATCTATC1070.1002050926662983No Hit
TCCTCCTCTGGCTAGAAACATCGCCCACGCAGGACCTTCTGTAGATCTAG1070.1002050926662983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)