FastQCFastQC Report
Tue 22 Apr 2025
Site5ff_S137_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5ff_S137_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420917
Total Bases81.6 Mbp
Sequences flagged as poor quality0
Sequence length23-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG325387.73026511164909No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC151423.597383807258913No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC119832.84687955107539No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG113282.6912669243580085No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG111312.6444643480781247No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA99402.361510701634764No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG95992.28049710512999No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG90192.1427027181130724No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA76491.8172228729179385No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG76021.8060567760389816No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA72641.725755909122226No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG69831.6589969043778228No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT58921.3998009108684135No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATACAGTTCGG56561.3437328499442882No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT47231.1220739480705222No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT41010.9743013468213448No Hit
GAATCAAAAGAGATGTTGGAATAGGACCGGGTCGCCTCCTCCTGAAGGAT40380.9593340254729554No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC40250.9562455305915417No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG36800.8742816279694097No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTAC36120.8581264239743227No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT33460.7949310671700122No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT31940.7588194347104061No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA29330.696811960552793No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT29200.6937234656713794No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA28450.6759052259709158No Hit
AAATCAAAAGAGATGTTGGAATAGGACCGGGTCGCCTCCTCCTGAAGGAT27950.6640263995039402No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA26450.6283899201030132No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT24940.5925158641727466No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT22200.52741989513372No Hit
GAACCAAAAGAGATGTTGGAATAGGACCGGGTCGCCTCCTCCTGAAGGAT21600.5131653033733492No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAATGATATGGTTTAA20480.48655673208732364No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG19580.46517484444676743No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT17620.41860984469622275No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTCCAGATTTAA16870.4007916049957593No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC16540.39295157952755533No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT16120.38297336529529574No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA16100.38249821223661673No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG15320.3639672429481347No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATGTGGTTTGC15300.36349208988945564No Hit
AAACCAAAAGAGATGTTGGAATAGGACCGGGTCGCCTCCTCCTGAAGGAT15280.3630169368307766No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA14650.3480496154823873No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA14470.343773237954276No Hit
GAATCAGAATAAATGCTGATAAAGGACAGGGTCTCCCCCCCCTCTCACAT13580.3226289268430593No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC13210.31383859525749735No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTTTAAATGATAAGGTTTGA12930.3071864524359909No Hit
GAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT12620.29982158002646603No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT12220.2903185188528855No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA12210.290080942323546No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC12090.2872300239714718No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11540.27416331485779855No Hit
CTACGGAAACCTTGTTACGACTTCACCTTTCTCTAAATGATAAGGTTTAG11480.2727378556817615No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT11450.27202512609374296No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG11340.2694117842710083No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAAATAGTGTGATTTAA10840.2575329578040326No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC10680.25373173333460036No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT10670.2534941568052609No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT10540.2504056619238472No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT10120.24042744769158766No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC9920.23567591710479738No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA9810.23306257528206273No Hit
AAATCAGAATAAATGCTGATAAAGGACAGGGTCTCCCCCCCCTCTCACAT9790.23258742222338372No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC9720.23092438651800712No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG9310.22118374881508707No Hit
GAATCAGAATAAGTGTTGGTATAGAATGGGATCTCCTCCTCCGGTAGGGT9280.22047101922706855No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAACGATGAGGTTTAA9210.21880798352169195No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT9070.21548191211093873No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT9040.2147691825229202No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT8920.21191826417084605No Hit
AAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT8850.21025522846546946No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT8320.19766367241047522No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT8260.19623821323443816No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT8080.1919618357063269No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA7990.18982364694227127No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG7830.18602242247283907No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG7730.18364665717944392No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG7490.1779448204752956No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA7460.17723209088727707No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT7390.17556905518190047No Hit
GAACCAGAATAAATGCTGATAAAGGACAGGGTCTCCCCCCCCTCTCACAT7250.17224298377114727No Hit
AAATCAGAATAAGTGTTGGTATAGAATGGGATCTCCTCCTCCGGTAGGGT7010.16654114706699896No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA6960.1653532644203014No Hit
GAACCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT6930.16464053483228286No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT6650.15798839201077647No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6530.1551374736587023No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6520.1548998971293628No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG6510.15466232060002327No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT6470.15371201448266522No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATAATCAAGTTTGA6430.15276170836530717No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT6380.15157382571860958No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG6360.1510986726599306No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6340.15062351960125156No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6340.15062351960125156No Hit
CTACGGAAACCTTGTTACTACTTTTACTTCCTCTAAATGCAACAGTTTGG6320.15014836654257252No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG6060.14397137677974517No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT5990.14230834107436857No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5980.14207076454502907No Hit
GAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT5910.14040772883965247No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATACAGTTCGG5800.13779438701691782No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTGC5790.1375568104875783No Hit
GAATCAAAATAGGTGCTGGTACAACACAGGGTCCCCCCCGCCGGCTACAT5770.13708165742889927No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5700.13541862172352268No Hit
AAACCAGAATAAATGCTGATAAAGGACAGGGTCTCCCCCCCCTCTCACAT5530.13137982072475096No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT5460.12971678501937436No Hit
CTACAGATACCTTGTGACGACTTAACCTACCTTGTCGTGAAAAGATTTTT5330.12662829013796068No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATAGCCAAGTTTGG5190.12330221872720751No Hit
AAACCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT5170.12282706566852848No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA5160.12258948913918898No Hit
GAACCAGAATAAGTGTTGGTATAGAATGGGATCTCCTCCTCCGGTAGGGT5110.1214016064924914No Hit
CTACATATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA5080.12068887690447284No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5060.12021372384579383No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4870.11569976978834307No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4810.11427431061230599No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4790.11379915755362696No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG4540.10785974432013913No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTCGT4530.10762216779079961No Hit
AAATCAAAATAGGTGCTGGTACAACACAGGGTCCCCCCCGCCGGCTACAT4510.1071470147321206No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT4480.10643428514410204No Hit
AAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4460.10595913208542303No Hit
AAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4420.10500882596806496No Hit
AAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT4370.10382094332136739No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4310.10239548414533031No Hit
CTACAGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC4310.10239548414533031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)