FastQCFastQC Report
Tue 22 Apr 2025
Site5ff_S137_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5ff_S137_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420917
Total Bases81.6 Mbp
Sequences flagged as poor quality0
Sequence length49-281
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT324387.706507458715138No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT128333.0488196010139768No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT117192.7841593473297586No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT82391.9573930252282516No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT81391.9336353722943005No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG80211.9056013418322377No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG76251.8115210362137903No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT73101.7366844294718438No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCGGGAGGGT63611.5112243031286454No Hit
CTACCGATTGAATGATTTAGTGAGGACTTCGGACTGGTGTCCGGGGCGGC63451.507423078659213No Hit
CTACCGATTGAATGGCTTAGTGAGACCTCCGGATTGGCTTCGGGGAGCCG59321.409303972041994No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG54981.3061957583086452No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG52451.2460888963857484No Hit
CTACCGATTGAATGGCTTAGTGAGATTTCCGGATTGGCGTTAGGAAGCCG51811.2308839985080196No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG51061.2130657588075557No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG45711.0859623156109162No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT43611.0360712444496185No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG42721.0149269333384014No Hit
CTACCGATTGAACGGTTTAGTGAATGCTCGGGATTGGCGCCTTGCTGCTG40510.962422520354369No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG40260.9564831071208812No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG38660.9184708624265592No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG37040.8799834646735579No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG36920.8771325463214839No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT35910.8531373168581929No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG34760.825816015984149No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG32970.7832898172323759No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA29800.7079780574317502No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC27240.6471584659208347No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG26400.6272020374563156No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG25960.616748670165377No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT25510.6060577263450989No Hit
CTACCGATTGAATGGTTCGGTGAACCTTTTGGACTGCTTAGGATTTATTC22750.5404866042473931No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT22100.5250441298403248No Hit
TCCTCCCTTATCCGGCAATCTTGCTCACTCAGGCCCATCAGTAGACATAG22060.5240938237229668No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT21770.5172041043721208No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTGCCTTT19710.4682633393281811No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG19670.467313033210823No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT19640.4666003036228045No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT19530.4639869618000699No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT19270.45780997203724255No Hit
CTACCGATCGAGTGTTTAGGTGAAATTGATAGACGTAAGGAAGTCAATTA18390.4369032374553653No Hit
CCCCCCTTATCCGGCAATCTTGCTCACTCAGGCCCATCAGTAGACATAGT18280.43428989563263065No Hit
CTACCGATTGAATGGTTTAGTGAGGTCTCCTGATCGGCGCTCTGCAGCTG17840.4238365283416921No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA17210.4088692069933027No Hit
TCCCCCCTTATCCGGCAATCTTGCTCACTCAGGCCCATCAGTAGACATAG17060.40530555905321003No Hit
CTTCCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACCGAGAGCAGCCCCG16950.4026922172304753No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGGGCCGG16580.39390188564491335No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTGCTTCGGCATGGCGTATT16060.38154790611925865No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC15650.37180726841633865No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA15630.3713321153576596No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT15410.3661054317121903No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT14860.35303872259851704No Hit
CTACCGATGAATGGTCCGGTGAGGACTTAGGATGGCCGCAGATCTTGGTG14790.35137568689314047No Hit
CTACCGACTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA14640.34781203895304774No Hit
CTAGCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT14580.34638657977701065No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGGCTTT14480.34401081448361553No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC14240.3383089777794672No Hit
TCCCCCTTATCCGGCAATCTTGCTCACTCAGGCCCATCAGTAGACATAGT13380.31787739625626904No Hit
CCCTCCCTTATCCGGCAATCTTGCTCACTCAGGCCCATCAGTAGACATAG13280.31550163096287387No Hit
CTACCGATTGAACGATTCGGTGAAATCTCGGGACCATGGTTGTCGCCTCA13100.31122525343476265No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGTCAGTTTGCTT13000.30884948814136753No Hit
CAACTGATTGAATGGTTCGGTGAAAAATTGGGACCACGAGTCTTTTTGCT12520.2974458147330709No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT12480.29649550861571283No Hit
TCCTCCTTATCCGGCAATCTTGCTCACTCAGGCCCATCAGTAGACATAGT12140.2884179066181694No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC11250.2672735955069527No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG11180.2656105598015761No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT11060.2627596414495019No Hit
CCCTCCTTATCCGGCAATCTTGCTCACTCAGGCCCATCAGTAGACATAGT10690.2539693098639399No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGTTTGGTTCCTT10350.24589170786639647No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG10330.24541655480771743No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG10070.23923956504489008No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT9880.23472561098743933No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG9720.23092438651800712No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG8950.2126309937588646No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT8780.20859219276009286No Hit
CTACCGATTGGCTGTGGGATAGAGCTGGACGGAGATGCTCGTTGGGGGCT8740.20764188664273478No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG8680.20621642746669772No Hit
CCCCCCCTTATCCGGCAATCTTGCTCACTCAGGCCCATCAGTAGACATAG8510.20217762646792597No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG8380.19908913158651229No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG8360.19861397852783327No Hit
TCCTCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG8260.19623821323443816No Hit
CTACCGATTGAATGGTTTAGTGAGACCTTGGGAGGGTGAGGGGGAGAGGG8080.1919618357063269No Hit
TCCTCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG8060.19148668264764787No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT7810.18554726941416003No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA7560.1796078561806722No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG7440.17675693782859803No Hit
TCCCCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG7420.176281784769919No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG7400.17580663171124No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG7380.17533147865256096No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA7170.17034237153643117No Hit
TCCCCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG7100.16867933583105457No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT7090.16844175930171507No Hit
CTACCGATTGAATGATCCGGTGAGCTTTTTGGACAGTGGCGCCGGTTGTG7040.1672538766550175No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG6850.16273992259756676No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG6820.16202719300954818No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAATGTTCAGC6800.16155203995086917No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGACGGTGCTCG6770.16083931036285062No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG6770.16083931036285062No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG6760.16060173383351112No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA6600.1568005093640789No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG6390.15181140224794912No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG6350.15086109613059107No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG6150.1461095655438008No Hit
CAACCGAGTTATGAAGGGATGAAGCTCTTTTTGAGAAAAATCTTTTCACG6140.14587198901446127No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG6110.14515925942644275No Hit
CCCTCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG6080.1444465298384242No Hit
CCCCCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG5920.140645305368992No Hit
CCCCCCTGAGAAGAAATGCAGCCCATGCAGGATCTTCTGTAGATTTTGCC5780.13731923395823878No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGTCACGGCACGGTG5650.13423073907682512No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG5630.1337555860181461No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG5380.12781617278465826No Hit
CCCTCCTCTAAGATTAAATGTCTCTCATTCGGGAGCTTCTGTGGATTGTG5260.12496525443258409No Hit
CTACCGATTGAATAGTTTAGTGAGGCACTTGGACTGGAACTCTCGAGGTT5140.12211433608050994No Hit
CTACCGATTTCGAGTGGTCCGGTGAGTCTTTCGGACTGCGCGCGGCTTCG5080.12068887690447284No Hit
CTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGGCTATTGGGAGCTC5010.11902584119909626No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG4940.11736280549371966No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG4910.11665007590570113No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG4810.11427431061230599No Hit
CCTCCGATTGAGTGATCCGGTGAATAATTCGGACTGGTGCTTTGTTTGGT4810.11427431061230599No Hit
CCCCCCTCTGGCTAGAAACATCGCCCACGCCGGCCCTTCTGTAGACCTGG4790.11379915755362696No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG4740.11261127490692939No Hit
TCCCCCTGAGAAGAAATGCAGCCCATGCAGGATCTTCTGTAGATTTTGCC4620.10976035655485523No Hit
TCCTCCCTGAGAAGAAATGCAGCCCATGCAGGATCTTCTGTAGATTTTGC4590.1090476269668367No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG4560.10833489737881814No Hit
TCCTCCTGAGAAGAAATGCAGCCCATGCAGGATCTTCTGTAGATTTTGCC4450.1057215555560835No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG4320.10263306067466982No Hit
TCCCCCCTGAGAAGAAATGCAGCCCATGCAGGATCTTCTGTAGATTTTGC4240.10073244843995371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)