FastQCFastQC Report
Tue 22 Apr 2025
Site5f_S96_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5f_S96_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8573
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length55-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG153517.90505074069754No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGCCTGGCGGAT5356.240522570862009No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT4615.3773474862941795No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT2562.986119211477896No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG770.8981686690773358No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG730.8515105563979938No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG690.8048524437186516No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG680.793187915548816No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT670.7815233873789805No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG660.769858859209145No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG640.746529802869474No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGTCTCGACATGGTG620.7232007465298029No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTTGGGAGGAT610.7115362183599674No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT570.6648781056806252No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG510.5948909366616121No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG490.571561880321941No Hit
CTACCGATTGGATGGTTTAGTGAGTTCCTCGGATTGGACCCTGCATGGTG440.5132392394727633No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG440.5132392394727633No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG420.48991018313309226No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT390.4549165986235857No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG380.4432520704537502No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG380.4432520704537502No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG380.4432520704537502No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG380.4432520704537502No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG380.4432520704537502No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG370.4315875422839146No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG340.396593957774408No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT330.3849294296045725No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG320.373264901434737No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG300.34993584509506587No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG300.34993584509506587No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG280.32660678875539484No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG280.32660678875539484No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT280.32660678875539484No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG270.3149422605855593No Hit
CCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG240.2799486760760527No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG240.2799486760760527No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG220.25661961973638164No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACGCTGCATGGTG220.25661961973638164No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC210.24495509156654613No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG210.24495509156654613No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG210.24495509156654613No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG200.2332905633967106No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG200.2332905633967106No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG180.20996150705703953No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG170.198296978887204No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG170.198296978887204No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT170.198296978887204No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT170.198296978887204No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT170.198296978887204No Hit
CTACCGCTTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG170.198296978887204No Hit
CCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG160.1866324507173685No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG160.1866324507173685No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT160.1866324507173685No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG160.1866324507173685No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATCGTG150.17496792254753293No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG140.16330339437769742No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT140.16330339437769742No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT140.16330339437769742No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT120.13997433803802636No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT120.13997433803802636No Hit
TCCCCCATTAGCAAGTATTGCATTCCACTCAGGGGGTGCAGTTGATTGTG120.13997433803802636No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC110.12830980986819082No Hit
CTACCGATTGGATGGTCCGGTGAGAATTTCGGATTGTGGTTGATTTGAGT110.12830980986819082No Hit
CTACCGATTGAGTGGTCCGGTGAACCCTCGGGATCGCATGCAATTTCTTT110.12830980986819082No Hit
CTACCGATCGAGTGTTTAGGTGAAATTGATAGACGTAAGGAAGTCAATTA110.12830980986819082No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG110.12830980986819082No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG100.1166452816983553No Hit
CCCTCCATTAGCAAGTATTGCATTCCACTCAGGGGGTGCAGTTGATTGTG100.1166452816983553No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCCTGGTG100.1166452816983553No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA100.1166452816983553No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG100.1166452816983553No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG100.1166452816983553No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA100.1166452816983553No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA100.1166452816983553No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGGGCCGG100.1166452816983553No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG90.10498075352851977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.10498075352851977No Hit
TCCTCCATTAGCAAGTATTGCATTCCACTCAGGGGGTGCAGTTGATTGTG90.10498075352851977No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCGTGGTG90.10498075352851977No Hit
CTACCGATTGAATGTCTTAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT90.10498075352851977No Hit
TCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG90.10498075352851977No Hit
CTACCGATTGGAGGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG90.10498075352851977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)