FastQCFastQC Report
Tue 22 Apr 2025
Site5ee_S136_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5ee_S136_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320100
Total Bases52.8 Mbp
Sequences flagged as poor quality0
Sequence length18-281
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG3406410.641674476726024No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG238827.460793502030616No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG233417.291783817557014No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC178965.590752889721962No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG163365.103405185879413No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG141804.429865666979069No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA96503.0146829115901284No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA87682.739144017494533No Hit
CTACGAAAACCTTGTTACGACTTTTACTTCCTCTAGATGATACAGTTTGA64482.01437050921587No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT56911.7778819119025304No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG49191.5367072789753202No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCTACAGTTCGG48561.5170259293970634No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC41271.289284598562949No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA40291.2586691658856608No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG35091.0962199312714778No Hit
CTACGGAAACCTTGTTACGACTTTAGCTTCCTCTAAATAACGAGGTTTAA34911.0905966885348328No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG26640.8322399250234301No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG25060.7828803498906591No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT24910.7781943142767884No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATATGGTTAGC19760.6173070915338956No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATACAGTTTGA18710.584504842236801No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAGATTTAT18710.584504842236801No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC17770.5551390190565448No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA17750.5545142143080287No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT16580.5179631365198376No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA15470.4832864729771946No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGA15420.4817244611059044No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT13440.4198687910028116No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATGTGGTTTAC13280.41487035301468295No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATAGCCAAGTTTGG12880.4023742580443611No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC10040.3136519837550765No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG9800.30615432677288346No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA9350.2920962199312715No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA9140.28553577007185255No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC8820.2755388940955951No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT8650.2702280537332084No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG8540.2667916276163699No Hit
CTACGATAACCTTGTTACGACTTTTTCTTCCTCCAATACCCAGCATTCAC8220.25679475164011245No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTCAC8070.2521087160262418No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG7970.24898469228366135No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT7920.24742268041237112No Hit
CTACGATAACCTTGTTACGACTTTTTCTTCCTCCAATACAAAGCATTCGC7710.2408622305529522No Hit
CTACGGAAACCTTGTTACGACTTCAGCTTCTTCTAAATAAATAGGTTTAA7400.23117775695095283No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6720.2099343955014058No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA6390.19962511715089035No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA6250.19525148391127772No Hit
GAATCAAAATAGATGTTGGTATAGGATAGGATCGCCACCTCCTGCGGGGT6170.19275226491721337No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT5740.17931896282411747No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG5700.1780693533270853No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG5460.17057169634489222No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5170.16151202749140894No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA4950.15463917525773196No Hit
GAATCAGAATAAATGTTGATATAGGATTGGGTCTCCCCCCCCCGAAGGGT4940.1543267728834739No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT4720.14745392064979693No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT4550.14214308028741018No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4530.1415182755388941No Hit
AAATCAAAATAGATGTTGGTATAGGATAGGATCGCCACCTCCTGCGGGGT4410.13776944704779756No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT4320.13495782567947517No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4310.13464542330521712No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC4280.13370821618244297No Hit
CTACGGATACCTTGTGACGACTTAACCCTTCTCACTGAACTTAAGTTTGG4170.13027179006560452No Hit
AAATCAGAATAAATGTTGATATAGGATTGGGTCTCCCCCCCCCGAAGGGT4160.12995938769134646No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4160.12995938769134646No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT3850.12027491408934708No Hit
GAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT3730.11652608559825056No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT3640.11371446422992816No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG3480.10871602624179945No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA3420.10684161199625117No Hit
GAACCAAAATAGATGTTGGTATAGGATAGGATCGCCACCTCCTGCGGGGT3330.10402999062792877No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3320.10371758825367074No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTTAATAAATAGATTTAC3310.10340518587941268No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC3250.10153077163386443No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3240.10121836925960637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)