FastQCFastQC Report
Tue 22 Apr 2025
Site5ee_S136_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5ee_S136_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320100
Total Bases52.7 Mbp
Sequences flagged as poor quality0
Sequence length59-281
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT3501810.939706341768197No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT239667.487035301468291No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT154744.834114339268979No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT143254.475164011246485No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT100813.1493283348953454No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT80252.5070290534208057No Hit
CTACCGATTGGATGATTTAGTGAGGCCCTCGGACTGGCTGCTGGTGGGGT68452.138394251796314No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT60101.8775382692908467No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA58871.8391127772571074No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG56221.7563261480787253No Hit
CTACCGATTGAGTGTTTTAGTGAGGTCCTCGGATTGTTTGCCTGGTGGCT50031.5629490784129958No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT49261.5388940955951265No Hit
CTACCGATTGAATGTCTCGGTGAAGACTTGGGACCGTGGTGCCATCGTTT39831.2442986566697907No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT34111.0656044985941893No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT33451.0449859418931584No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCCTTG32441.013433302093096No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG31900.9965635738831614No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT31610.9875039050296783No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTG30340.9478288034989065No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT27780.8678537956888474No Hit
CTACCGATTGAATGGCTTAGTGAGCTCGAGGGACTGTTGCCGCTTGAGGG26380.8241174632927211No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG24410.7625741955638863No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT24350.760699781318338No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG23690.7400812246173071No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCGCGGCG22350.6982193064667291No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATCGGCGCTCTGGAGAAT22350.6982193064667291No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG21630.6757263355201499No Hit
CTACCGATTGAATGGTTCGGTGAGTTATCTGGACTGCTGCGTTCTTCAAG21480.6710402999062793No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG20510.640737269603249No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG19800.6185567010309279No Hit
CTACCGATTGAATGGTTTGATGAATTGCGCGGACTATCCGTCTTGGGGGT18940.5916900968447361No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT18720.5848172446110591No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG17790.5557638238050608No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT17640.5510777881911902No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG17170.5363948766010621No Hit
CTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGGTTTAAAGAAGGGG16610.5189003436426117No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG16260.5079662605435802No Hit
CTACCGATTGGATGATTTAGTGAGAACTTCGGACTGTCTGCTTGTGGGGC16060.5017182130584192No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGCCTCGTTGG15940.4979693845673227No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG15630.48828491096532334No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGCGTCTCGT15550.485785691971259No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG15100.47172758512964696No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA14030.43830053108403627No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT13450.42018119337706966No Hit
CTACCGATTGAATAGTTTAGTGAGGCACTTGGACTGGAACTCTCGAGGTT13360.4173695720087473No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG12600.39362699156513586No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG12580.39300218681661986No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC10720.33489534520462355No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT10240.3199000312402374No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT8640.26991565135895035No Hit
CTACCGATTGAGCAATCCGGTGAATAATTCGGACCGATAGACTACCTGGT8380.26179318962824116No Hit
CTTCCGACTGATACTCATTGTGAATTACGGGGATGTAACAAAAACCGTTG8090.2527335207747579No Hit
CTACTGATTGAATACATCGGTGAAATATTGGGAATGTGATTTTTAAAGCT7500.23430178069353325No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA6910.21587004061230866No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT6700.2093095907528897No Hit
CTTCCGACTGATACTCATTGTGAGTTGCAAGGATTCATTTTTGAAGAATT6350.19837550765385817No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG6290.19650109340830993No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5530.17275851296469852No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGCTAGGACTTG5490.17150890346766637No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGTACTTTGGCATGGAACTTT5270.16463605123398936No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG5230.16338644173695718No Hit
TCCTCCACTATCAAGAAATCTAGCACATGCCGGTCCATCTGTAGACCTTG4870.1521399562636676No Hit
TCCCCCACTATCAAGAAATCTAGCACATGCCGGTCCATCTGTAGACCTTG4670.14589190877850672No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG4530.1415182755388941No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG4350.1358950328022493No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTACTTGGTGGGGCT4280.13370821618244297No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG4170.13027179006560452No Hit
CACTCGAGCAGTGCTTAAGTGAGTCATAGCCTTTTTAGTTATGCCAAACT4130.12902218056857231No Hit
CCCTCCACTATCAAGAAATCTAGCACATGCCGGTCCATCTGTAGACCTTG4100.1280849734457982No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG4080.1274601686972821No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG4050.12652296157450796No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGGTTTAGTTCCTT3870.12089971883786317No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT3740.11683848797250859No Hit
CTACCGATTGAGTGATCCGGTGAATACTTCGGACTGCAGCAGTGTTCGGA3730.11652608559825056No Hit
CTACCGATTCAATGAATCGATGAACTGGATGGAGTGGCCTTATTAGGGGC3660.11433926897844422No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3650.11402686660418619No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG3640.11371446422992816No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG3580.11184004998437988No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC3570.11152764761012185No Hit
CCCCCCACTATCAAGAAATCTAGCACATGCCGGTCCATCTGTAGACCTTG3400.10621680724773508No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGATCTTGGGAGGTT3310.10340518587941268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)