FastQCFastQC Report
Tue 22 Apr 2025
Site5e_S95_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5e_S95_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347948
Total Bases53.1 Mbp
Sequences flagged as poor quality0
Sequence length43-281
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG10783430.99141250991527No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT330039.485037994183038No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT174845.024888776483842No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAGCTCCGTGGCTT95662.749261383884948No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT94262.7090254865669583No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGTCTCGACATGGTG91712.6357386735949047No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGAATTAGGTTAGTT63571.826997137503305No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACCGGACGCTGGCTTCGG56861.63415222964351No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT51441.478381827169577No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT45301.301918677503535No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT37791.0860818283191742No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG36821.058204099463138No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT32270.9274374331796705No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT24150.6940692287353283No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT22850.656707324082909No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT17110.4917401450791498No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC17070.49059054801292146No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG16780.4822559692827664No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG14430.4147171416418545No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT13970.40149677538022926No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT12770.3670088633933806No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA11690.3359697426052169No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT11110.3193005851449067No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGGTTTAGTTCCTT10230.2940094496878844No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG9490.2727419039626611No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGGCGCCATCGTGGCT8670.24917516410498122No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT8520.24486417510662514No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT8450.2428523802407256No Hit
CTACCGATTGGATGGTTTAGTGAGTTCCTCGGATTGGACCCTGCATGGTG8400.2414153839079403No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT8140.23394300297745638No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT7260.20865186752043408No Hit
CTACCGATTGAGTGATTTAGTGAGGTCTTGGGAGGATCTCTGTTGAAGGG7130.20491567705519217No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC7080.20347868072240677No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGACCCGTTTTGGAG6680.1919827100601239No Hit
CTACCGATCGAGTGTTTAGGTGAAATTGATAGACGTAAGGAAGTCAATTA6520.18738432179521078No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC6120.1758883511329279No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCTCGCCC6010.1727269592008001No Hit
CTACCGATCGAGTAGTGCACTGAGACCATTGGATCCGTACCGGGCGGTGC5820.16726637313621576No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTTGGGCCGG5650.16238058560474555No Hit
CTACCGATTGAATGGTTTAGTGAGACCCTCGGATTCGTCCCAGCAGGGTG5640.16209318633818845No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCCCGCCC5370.15433340614114752No Hit
CTACCGATTGGATGGTCCGGTGAGAATTTCGGATTGTGGTTGATTTGAGT5300.15232161127524804No Hit
CTACCGATTGAGTGGTCCGGTGAACCCTCGGGATCGCATGCAATTTCTTT5290.15203421200869094No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA5260.15117201420901974No Hit
CTACCGATTGAATGGCTTAGTGAGCTTTCTGGACTGTTGCGCTTCGTTGG5070.14571142814443538No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG5040.14484923034476416No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG5000.14369963327853588No Hit
CTACCGATTGAATGATCCGGTGAGGCCCCCGGACTGGGGCGACGCATTCG4990.1434122340119788No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG4980.14312483474542173No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCGT4910.14111303987952223No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGGGCCGG4830.13881384574706565No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG4750.13651465161460907No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG4680.13450285674870957No Hit
TACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTGG4570.1313414648165818No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA4520.12990446848379644No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG4490.12904227068412522No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA4270.12271948681986963No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT3880.11151091542414383No Hit
CTACCGATTGAATGATTCGGTGAAAATTTCGGACCGTGCTGCTGTGGCCT3820.1097865198248014No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG3760.10806212422545898No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA3750.10777472495890192No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATAGATGGACTGTG3660.10518813155988825No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT3630.10432593376021704No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGACCGGCCCCGGCACGGCG3590.10317633669398875No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG3540.10173934036120338No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT3490.10030234402841805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)