FastQCFastQC Report
Tue 22 Apr 2025
Site5cc_S134_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5cc_S134_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423224
Total Bases77.2 Mbp
Sequences flagged as poor quality0
Sequence length21-281
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG6076314.357172561102393No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA5246612.396744986106647No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG244345.77330208116742No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG102902.4313365971684027No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA93582.211122242594938No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC83801.980038939190594No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG75071.7737651929002136No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT62491.476523070525301No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT42651.0077405818195566No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT40140.9484339262423681No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC39180.925750902595316No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG37100.8766043513600363No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT34480.8146985993232898No Hit
CTACGGAAACCTTGTTACGACTTCAGCTTCTTCTAAATAAATAGGTTTAA34150.8069013099446156No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCACCAGTTCGG30850.7289284161578738No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG29000.6852163393380337No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG28670.6774190499593596No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT21380.5051698391395574No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA20630.4874487269152978No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA16970.40096969926091147No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA16800.3969529138234127No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA15140.3577301854337183No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT14960.35347711849989605No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT14480.34213560667636994No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT14000.3307940948528439No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA13220.31236413813961406No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAA12590.29747840387123603No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA12290.2903899589815322No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG12080.2854280475587396No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG11720.27692191369109503No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG11250.2658166833638924No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGATAAGGTTTAC10830.2558928605183071No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT10270.24266109672419334No Hit
CTACGATAACCTTGTTACGACTTTTTCTTCCTCCAATACAAAGCATTCGC10180.2405345632572822No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT9810.2317921478933142No Hit
AAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT9810.2317921478933142No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA9760.2306107404116969No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT9680.22872048844110918No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA9660.22824792544846226No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8900.21029053172787932No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT8590.20296580534185207No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC8330.19682248643744213No Hit
AAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAA8230.1944596714742075No Hit
GAACCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT7820.18477213012494564No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA7760.1833544411470049No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT7730.1826455966580345No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA7690.18170047067274067No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC7630.18028278169479992No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA7580.17910137421318262No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGA7510.1774474037389184No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT7340.17343061830141956No Hit
GAACCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAA7200.17012267735289113No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT7170.16941383286392075No Hit
GAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT6870.16232538797421697No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAA6760.1597262915146589No Hit
GAATCAAAATAGGTGCTGGTACAACACAGGGTCCCCCCCGCCGGCTACAT6730.15901744702568854No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT6520.15405553560289587No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG6420.15169272063966127No Hit
GAATCAGAATAAATGCTGATATAAAATTGGATCTCCTCCTCCTCTAGCAT6240.147439653705839No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA6220.14696709071319208No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGTTGATCAAGTTTGA5900.13940608283084135No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGAAATTCAGTTTGA5700.13468045290437214No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA5480.12948225998525603No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA5350.12641060053305106No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAAAAA5350.12641060053305106No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5290.12499291155511029No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT5190.1226300965918757No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT5120.12097612611761148No Hit
AAACCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT5050.11932215564334726No Hit
GAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT4990.11790446666540651No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4960.11719562217643613No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGATTTAC4960.11719562217643613No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGT4770.11270627374629037No Hit
GAATCAAAATAAATGTTGGTATAAAATTGGATCACCTCCACCTGCGGGAT4690.1108160217757027No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC4620.10916205130143848No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG4610.10892576980511502No Hit
AAATCAAAACAAGTGCTGGTAAAGAACAGGATCTCCGCCTCCCCCAACGT4600.10868948830879156No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT4420.10443642137496928No Hit
AAATCAGAATAAATGCTGATATAAAATTGGATCTCCTCCTCCTCTAGCAT4400.10396385838232236No Hit
TACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAGA4390.1037275768859989No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATAAGGTTTAG4370.10325501389335198No Hit
AAATCAAAATAGGTGCTGGTACAACACAGGGTCCCCCCCGCCGGCTACAT4340.1025461694043816No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAC4330.10230988790805814No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCCCCAACAA4240.10018335444114701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)