FastQCFastQC Report
Tue 22 Apr 2025
Site5bb_S133_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5bb_S133_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378651
Total Bases74.4 Mbp
Sequences flagged as poor quality0
Sequence length27-281
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG5106013.484712835830356No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA4352811.495546030513587No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG205565.428745731557556No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG82912.189615239362896No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA76292.0147840623687774No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC61331.6196972938140926No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG53411.4105337104616125No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT44151.1659813390166671No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT38401.0141264647392982No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT36330.9594587099994454No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT35850.9467821291902042No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT31500.8319006156064556No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG31390.8289955658376711No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT30480.8049628813868179No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC30380.8023219270515594No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT29710.7846275330053267No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT28320.7479182677452324No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT26390.6969478490747416No Hit
CTACGGAAACCTTGTTACGACTTCAGCTTCTTCTAAATAAATAGGTTTAA25710.6789893595949833No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT25490.6731792600574144No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCACCAGTTCGG25390.6705383057221558No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT23870.6303957998262252No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT23520.6211524596528202No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT23500.6206242687857684No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG23160.6116450240458893No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG22470.593422439132605No Hit
GAATCAAAATAAGTGTTGGTAGAGAATGGGGTCGCCTCCCCCCAAAGGGT21440.5662206094794415No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT21100.5572413647395623No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT20520.5419238295950625No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT19930.5263421990170368No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT19610.5178911451442093No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT18340.48435102508642525No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT17980.47484358947949434No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT17870.47193853971070987No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT16790.4434162328899171No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA16450.43443698815003784No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT16230.428626888612469No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT15390.4064428721962969No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT15340.40512239502866754No Hit
AAATCAAAATAAGTGTTGGTAGAGAATGGGGTCGCCTCCCCCCAAAGGGT14800.3908612416182712No Hit
AAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT13980.36920541606915075No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA13960.36867722520209906No Hit
AAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT13870.3663003663003663No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT13790.36418760283215945No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA13660.3607543621963233No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT13600.3591697895951681No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT13540.35758521699401297No Hit
GAACCAAAATAAGTGTTGGTAGAGAATGGGGTCGCCTCCCCCCAAAGGGT11950.3155940430634014No Hit
AAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT11870.3134812795951945No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11840.31268899329461697No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA11650.3076711800576256No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT11480.303181557687686No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA10360.2736028691327898No Hit
GAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT10200.2693773421963761No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA10190.2691132467628502No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG9620.25405980705187625No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG9590.25326752075129866No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT9560.2524752344507211No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG9380.24772151664725567No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT9370.2474574212137298No Hit
AAACCAAAATAAGTGTTGGTAGAGAATGGGGTCGCCTCCCCCCAAAGGGT8390.22157606872819563No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGATAAGGTTTAC8230.2173505417917819No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7910.20889948791895438No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT7770.20520215184959234No Hit
CTACGATAACCTTGTTACGACTTTTTCTTCCTCCAATACAAAGCATTCGC7700.20335348381491136No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA7550.19939205231202348No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT7530.19886386144497176No Hit
AAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7210.19041280757214427No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA7030.18565908976867881No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC6900.18222584913284265No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6460.17060565005770487No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA6450.170341554624179No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA6420.16954926832360143No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGA5800.15317535144499816No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA5700.15053439710973957No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC5690.15027030167621372No Hit
AAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT5600.147893442774481No Hit
GAATCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT5450.1439320112715931No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGTTGATCAAGTTTGA5440.14366791583806723No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT5340.14102696150280866No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG5290.13970648433517935No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT5270.13917829346812766No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAA5040.1331040984970329No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA4970.13125543046235189No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT4890.129142666994145No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4830.12755809439298985No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGAAATTCAGTTTGA4530.11963523138721409No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA4280.1130328455490676No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT4200.11092008208086074No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAC4180.11039189121380903No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA4170.11012779578028317No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGATTTAC4120.10880731861265386No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4080.10775093687855042No Hit
AAATCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT3980.10510998254329185No Hit
TACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAGA3960.10458179167624013No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3910.10326131450861083No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3880.10246902820803326No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTAA3800.10035626473982638No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC3790.10009216930630054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)