FastQCFastQC Report
Tue 22 Apr 2025
Site5bb_S133_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5bb_S133_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378651
Total Bases74.4 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG3988410.533182270745357No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT245596.4859197519615694No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT198255.235691969650152No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG155354.102722559824218No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG137763.6381786922522323No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG109282.8860348975705863No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG105082.7751148154897254No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT101002.667363878611175No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT93252.4626899176286345No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT78722.078959252715561No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT54241.4324536314442586No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG34380.9079601004619029No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGTCTCGACATGGTG33230.8775891256064292No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG31540.832956997340559No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA30980.8181676530631109No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG30850.8147344124272747No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG28920.7637639937567839No Hit
CTACTGATTGAATACATCGGTGAAATATTGGGAATGTGATTTTTAAAGCT26060.6882326997683883No Hit
CTACCGATTGGGTGTACCAGTGAGAGCCTTGGACTGGCGGCCGTTGCCTT25840.6824226002308195No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG25810.6816303139302419No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG23700.6259061774562856No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG23550.6219447459533978No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT23040.6084758788435789No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG21590.5701820409823294No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG21370.5643719414447604No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT21350.5638437505777087No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA20530.5421879250285884No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT19740.5213243857800455No Hit
TCCTCCTCTAAGATCAAGATTAGGGCACCCCGGAGGTGCCGTTGACCTAG17780.4695616808089771No Hit
TCCCCCTCTAAGATCAAGATTAGGGCACCCCGGAGGTGCCGTTGACCTAG16390.43285241554888276No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG15160.40036867722520214No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT14460.38188199687839197No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG14080.3718463704044093No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG13980.36920541606915075No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG13360.3528314991905475No Hit
CCCCCCTCTAAGATCAAGATTAGGGCACCCCGGAGGTGCCGTTGACCTAG12930.3414753955489356No Hit
CCCTCCTCTAAGATCAAGATTAGGGCACCCCGGAGGTGCCGTTGACCTAG12830.33883444121367695No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGATCTTGGGAGGGC12410.3277424330055909No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT11750.3103121343928842No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTACTTGGTGGGGCT11580.30582251202294464No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT11540.3047661302888412No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG10760.2841666864738242No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG10610.2802052549709363No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG10050.2654159106934882No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT8420.22236835502877317No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT8140.21497368289004914No Hit
CTACCGATTGAGTGGTCCGGTGAACCCTCGGGATCGCATGCAATTTCTTT7590.2004484340461269No Hit
CTACCGATTGAATGGCTTAGTGAGCTTTCTGGACTGTTGCGCTTCGTTGG7450.1967510979767649No Hit
TCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG6910.1824899445663685No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG6730.17773622676290304No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT6170.16294688248545494No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATCGTGGCGAGTTTCCTT5830.15396763774557573No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGACCGTGACTTTGATTTGT5540.14630887017332583No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC5530.1460447747398No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT5520.1457806793062741No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT5220.13785781630049834No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC5210.1375937208669725No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGACCCGTTTTGGAG5180.1368014345663949No Hit
CTACCGATTGAGTGATTTAGTGAGGTCTTGGGAGGATCTCTGTTGAAGGG4930.13019904872824845No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGGTTTAGTTCCTT4810.12702990352593813No Hit
TCCCCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG4790.1265017126588864No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATTGTGGTCTGTTTCCTT4760.12570942635830884No Hit
CTTCCGACTGATACTCATTGTGAGTTGCAAGGATTCATTTTTGAAGAATT4690.12386075832362783No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC4610.12174799485542095No Hit
CTACCGATTGAATCATTCGGTGAGGATCTCGGATTTTGTTGCTTACGCTT4590.12121980398836923No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT4470.11805065878605892No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4450.11752246791900721No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG4410.11646608618490377No Hit
CTACCGATTGAATGGTTTAGTGAGGTCATCGGATTGGGCGCATGAGAGTG4290.11329694098259346No Hit
CCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG4070.10748684144502457No Hit
CCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAGC4050.10695865057797287No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3900.10299721907508497No Hit
CCCCCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG3860.10194083734098153No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCTGGTTTCCTT3850.10167674190745568No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT3820.10088445560687809No Hit
CTACCGATTGAACGGCTTAGTGAGTTGTAGGGACCGGAGTCTGCGCGGGG3800.10035626473982638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)