FastQCFastQC Report
Tue 22 Apr 2025
Site5b_S88_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5b_S88_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200345
Total Bases33.9 Mbp
Sequences flagged as poor quality0
Sequence length50-281
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT3858519.259277745888344No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT2235111.15625545933265No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT119205.9497367041852804No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT57502.8700491651900473No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT46342.313010057650553No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG34051.6995682447777585No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG30521.523372182984352No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG30091.5019092066185828No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT25531.2743018293443809No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG22381.1170730489904914No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG22291.1125807981232374No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTTGGGAGGAT17930.8949562005540442No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG17490.8729940852030247No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG15930.7951284035039557No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG15810.7891387356809504No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG13220.6598617385010855No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG12980.6478824028550749No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG11960.5969702263595298No Hit
CTACCGATTGAATGATTTAGTGAGGCCTCCGGACTGGCGTTCCTGGCGGC10770.537572687114727No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACCGGACGCTGGCTTCGG10500.5240959345129651No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT8770.43774489006463846No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA7880.3933215203773491No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG7790.3888292695100951No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT7460.3723576829968305No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG7090.35388954054256405No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG6990.34889815069005964No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG6500.32444034041278796No Hit
CTACCGATTTCGAGTGATTCGGTGAACCTTCTGGACCGTGTGAATCCTTG6290.31395842172252864No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT5890.2939928623125109No Hit
CCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG5810.2899997504305074No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG5540.2765229978287454No Hit
CCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG5260.26254710624173305No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG5140.2565574384187277No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4890.24407896378746663No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT4720.2355936010382091No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT4420.22061943148069582No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4240.2116349297461878No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA4200.20963837380518605No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGGTTTAGTTCCTT3870.19316678729192144No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT3850.19216850932142054No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3820.19067109236566923No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3610.18018917367540993No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT3550.17719433976390725No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3500.17469864483765504No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT3490.1741995058524046No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3480.17370036686715415No Hit
CTACCGATCGAGTGTTTAGGTGAAATTGATAGACGTAAGGAAGTCAATTA3470.1732012278819037No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCGT3300.1647158651326462No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC3040.15173825151613465No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG2930.1462477226783798No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2810.1402580548553745No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG2750.13726322094387183No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCTCGTGGCTTCG2750.13726322094387183No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT2720.1357658039881205No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG2710.13526666500287005No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC2670.13327010906186829No Hit
CTACCGATTGGATGGTCCGGTGAGAATTTCGGATTGTGGTTGATTTGAGT2570.12827871920936385No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG2550.12728044123886295No Hit
CTACCGATTGAATGATCCGATGAATATCGTGGACTGAGGGTGTAAGCGGG2550.12728044123886295No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT2500.12478474631261074No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2480.12378646834210985No Hit
GTATCGATTGAGTGATCCGGTGAACTTTCTGGACTGCGACCAATCTCGAA2400.11979335646010632No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG2310.11530110559285234No Hit
TCCTCCTTTATCTGCAGGAATTGCACATGCTGGGGCTTCTGTAGATATAG2300.11480196660760189No Hit
TCCTCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA2290.11430282762235144No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG2290.11430282762235144No Hit
TCCCCCTTTATCTGCAGGAATTGCACATGCTGGGGCTTCTGTAGATATAG2260.11280541066660012No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA2200.10981057675509746No Hit
CTACCGATTGAATGTCATTGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT2200.10981057675509746No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGCTTGGT2190.10931143776984702No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCCTCG2140.10681574284359481No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG2090.10432004791734258No Hit
CTACCGATTGAGTGGTCCGGTGAACCCTCGGGATCGCATGCAATTTCTTT2040.10182435299109036No Hit
TCCCCCGTCTCGTGATTCTTCGGCCACCCCGAAGTCTACTGTCTCTTATA2010.10032693603533903No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)