FastQCFastQC Report
Tue 22 Apr 2025
Site5aa_S132_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5aa_S132_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310549
Total Bases57 Mbp
Sequences flagged as poor quality0
Sequence length26-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG4515814.541344522120504No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA3225810.387410682372186No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG99943.2181716894918355No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG95463.0739110414137545No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA86432.783135672631372No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG67792.182908333306499No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC60881.9603991640610658No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG43141.389152758501879No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG35361.1386286866162827No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG30790.9914699451616332No Hit
CTACGGAAACCTTGTTACGACTTCAGCTTCTTCTAAATAAATAGGTTTAA30630.9863177791588444No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC28130.9058151853652725No Hit
GAATCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT25150.8098560935633347No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT25010.8053479483108946No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT24540.7902134606777029No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG24260.781197170172823No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT22740.7322515931463311No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA20220.6511049786024106No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT19610.631462345716779No Hit
AAATCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT19550.6295302834657333No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT18240.5873469243179015No Hit
CTACGAAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA17980.57897465456337No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT17230.5548238764252984No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT16990.5470956274211155No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA15880.5113524757767696No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT15470.4981500503946237No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT14630.47110117887998354No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT14270.4595088053737092No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT13100.42183359147831745No Hit
GAACCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT13020.4192575084769231No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT12580.4050890519692545No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT12460.401224927467163No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG11830.38093827383118284No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA11780.37932822195531135No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA11490.369989921075257No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT11330.36483775507246846No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA11260.36258368244624845No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT10960.35292337119101974No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG10840.3490592466889283No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT10640.34261903918544256No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTCGA10530.3390769250585254No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT10150.3268405308019024No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT10020.3226543959246367No Hit
AAACCAGAATAAATGTTGGTATAAAACTGGGTCTCCACCACCTGCAGGGT9800.31557016767080237No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT9450.3042998045397023No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT9430.30365578378935365No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG9180.2956055244099965No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT9030.2907753687823822No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA8900.2865892339051164No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT8870.2856232027795935No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA8100.2608284038911734No Hit
AAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7900.2543881963876876No Hit
AAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7890.2540661860125133No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA7870.25342216526216477No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7230.2328135012510103No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT7100.2286273663737446No Hit
AAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7060.2273393248730474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6810.21928906549369018No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC6480.2086627231129387No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGATAAGGTTTAC6420.20673066086189296No Hit
CTACGATAACCTTGTTACGACTTTTTCTTCCTCCAATACAAAGCATTCGC6370.20512060898602155No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA6320.2035105571101501No Hit
CTACGGAAACCTTGTTACGACTTTTGTTTCCTCTAAGTGCAAAGGTTTAC6270.20190050523427863No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAA6180.19900241185771003No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT6120.19707034960666434No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6070.19546029773079288No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGA5890.18966411097765568No Hit
GAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5860.18869807985213283No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA5740.18483395535004138No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA5730.1845119449748671No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA5720.1841899345996928No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGATTTAC5710.18386792422451853No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT5640.1816138515982985No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT4970.1600391564616212No Hit
AAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT4940.15907312533609833No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC4820.15520900083400688No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4790.154242969708484No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA4640.14941281408086968No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA4540.1461927103291268No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT4460.1436166273277325No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAC4400.14168456507668675No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAAGGGTTAGGTTTAG4360.14039652357598964No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGTTGATCAAGTTTGA4300.1384644613249439No Hit
CTACGGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTAC4240.13653239907389816No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG4080.13138023307110955No Hit
AAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4080.13138023307110955No Hit
GAATCAAAACAAATGCTGGTAAAGAACTGGATCACCACCACCTGCTGGAT4030.12977018119523812No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGAAATTCAGTTTGA4030.12977018119523812No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTAA3910.12590605669314667No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA3720.11978785956483518No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT3710.11946584918966088No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG3650.11753378693861516No Hit
CTACGGCTACCTTGTTACGACTTCACCCCAGTCGCTGATCCTACCGTGGC3600.11592373506274373No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC3490.11238162093582654No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT3460.1114155898103037No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTAAGGTTCAC3440.11077156905995511No Hit
GAACCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT3370.10851749643373511No Hit
CTACGGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCGCTGCGATTAA3310.10658543418268936No Hit
CTACGATAACCTTGTTACGACTTTTTCTTCCTCCAATACGAAGCATTCGC3270.1052973926819922No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC3230.10400935118129508No Hit
TACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAGA3190.10272130968059792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)