FastQCFastQC Report
Tue 22 Apr 2025
Site5aa_S132_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5aa_S132_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310549
Total Bases57 Mbp
Sequences flagged as poor quality0
Sequence length52-281
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG301759.716663070884143No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT230377.418153012890075No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT101403.265185204267281No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT97253.131550898569952No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT91682.9521911195978734No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG91642.950903078097176No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG79842.5709308353915166No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG63772.0534601624864353No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG61761.9887360770764035No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT61501.9803638073218717No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT57531.8525256883776795No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT43831.411371474388905No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT35791.152475132748777No Hit
CTACTGATTGAATACATCGGTGAAATATTGGGAATGTGATTTTTAAAGCT31131.0024182979175589No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA25370.8169403218171689No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGTCTCGACATGGTG25010.8053479483108946No Hit
TCCTCCTTTATCCGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG21700.6987625141282052No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG21140.6807299331184451No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA21130.6804079227432708No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT20230.6514269889775849No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG19810.6379025532202648No Hit
TCCCCCTTTATCCGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG19390.6243781174629447No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT19300.621480024086376No Hit
CTACCGATCGGATGGTTTAGTGAGACCCTCGGACTGGTCCCGACGGGGCA19140.6163278580835875No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACTGGGCCTTTGGAGGTT18480.5950751733220844No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT18060.5815507375647644No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG17340.5583659905522156No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTACTTGGTGGGGCT16450.529707067161704No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG14950.4814055108855607No Hit
CCCTCCTTTATCCGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG14800.4765753552579464No Hit
CCCCCCTTTATCCGGACCACAAATGCACTCAGGAGGTTCTGTAGATATGG14280.4598308157488834No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG13850.44598436961638904No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG13360.43020586123284893No Hit
CTACCGATTGAATGGTTTAGTGAGATGTTCGGATTGGCGCCACTGCTCTT11780.37932822195531135No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG11780.37932822195531135No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT11510.37063394182560566No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT11490.369989921075257No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCCCGCCC10570.34036496655922255No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG9870.31782424029702233No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT8890.2862672235299421No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG8840.2846571716540707No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT8530.27467485002366776No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG8340.2685566528953563No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG7910.25471020676286193No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTTGGGATCGTGGCGAGTTTCCTT7630.24569391625798184No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT7140.2299154078744417No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGACCGTGACTTTGATTTGT6800.2189670551185159No Hit
TTACCGATTGAGTGTTAAGGTGAACCTTCTTGATAGTGTCAAAACTAAAT6350.20447658823567294No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6330.20383256748532438No Hit
CTACCGATTGAATGGCTTAGTGAGCTTTCTGGACTGTTGCGCTTCGTTGG6320.2035105571101501No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5860.18869807985213283No Hit
TCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG5810.1870880279762614No Hit
CTACCGATTGAGTGGTCCGGTGAACCCTCGGGATCGCATGCAATTTCTTT5620.18096983084794993No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGATCTTGGGAGGGC5370.17291957146859271No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGACCCGTTTTGGAG5140.16551333283958408No Hit
CTACCGATTGAATGATCCGATGAATATCGTGGACTGAGGGTGTAAGCGGG5030.16197121871266693No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT5030.16197121871266693No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC4960.1597171460864469No Hit
CTACCGATTGAATCATTCGGTGAGGATCTCGGATTTTGTTGCTTACGCTT4400.14168456507668675No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT4400.14168456507668675No Hit
TTACCGATTGAGTGTTCAGGTGAACCTTCTTGATAGTGTTCGCATTAAAA4360.14039652357598964No Hit
CTACCGATTGAGTGATTTAGTGAGGTCTTGGGAGGATCTCTGTTGAAGGG4100.13202425382145813No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTTGGGCCGG4080.13138023307110955No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT4080.13138023307110955No Hit
CAACCGATTGAATGGTTCGGTGAAGAATCTGGACACGAGGATGGTGGGAT4040.1300921915704124No Hit
TCCCCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG4020.12944817082006382No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCTGGTTTCCTT3980.12816012931936666No Hit
CTACCGATTGAACGGCTTAGTGAAATGTGAGGACCGAGGGGTGTGCAGGG3830.12332997369175235No Hit
CTACCGATTGAGTGGTCCGGTGAGGCCTTGGGAGGGATAGATGGACTGTG3760.12107590106553233No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG3740.12043188031518376No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGCGGTTTAGTTCCTT3610.116245745437918No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT3590.11560172468756945No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC3520.11334765206134942No Hit
CCCTCCGCTTTCAAGTGGAACTTCTCACTCAGGTGGTGCTGTTGATCTAG3520.11334765206134942No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGTTTAGTTCCCTT3420.11012754830960654No Hit
CTACCGATTGAACGGCTTAGTGAGTTGTAGGGACCGGAGTCTGCGCGGGG3420.11012754830960654No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC3350.10787347568338651No Hit
CTACCGATTGAGTGGTCCGGTGAATTAACCAGGTTTCGGTTGTGGCAGTT3330.10722945493303795No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCGT3320.10690744455786366No Hit
CCCGGAACTGAGCCGTTTCGAGAAATGTGGGGACCGTTGATTAGCTGGTC3190.10272130968059792No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT3190.10272130968059792No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG3190.10272130968059792No Hit
CTACCGATTGAATGGTCCGGTGAAGTCTTCGGACTGCAGCTGCCTTGAGC3160.10175527855507505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)