FastQCFastQC Report
Tue 22 Apr 2025
Site5L_S109_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5L_S109_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186022
Total Bases35.2 Mbp
Sequences flagged as poor quality0
Sequence length52-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG2908615.635785014675681No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG157068.44308737676189No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG107365.771360376729634No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC103145.544505488598123No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG66233.5603315736848327No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT57853.1098472223715476No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG45632.4529356742750856No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG40782.1922138241713345No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG34081.8320413714506885No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT21001.1288987324079947No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA15430.829471782907398No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT14580.7837782627861221No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT11900.6397092816978637No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC11800.6343335734483018No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT9630.517680704432809No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG9480.5096171420584662No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA9420.506391717108729No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT7590.40801625614174664No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT6770.36393544849533926No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT6650.357484598595865No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA6020.3236176366236252No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT5880.31609164507423854No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA5840.3139413617744138No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT5440.2924385287761662No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG5150.2768489748524368No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT5070.2725484082527873No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5040.27093569577791876No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA4990.2682478416531378No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4580.2462074378299341No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGATAAGGTTTAC4490.2413693004053284No Hit
GAATCAAAATAAATGTTGGTATAAAATTGGATCACCTCCACCTGCGGGAT4470.24029415875541604No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG4420.2376063046306351No Hit
GAATCAGAATAAATGCTGGTAGAGAATAGGGTCTCCACCTCCAGCAGGGT4280.23008031308124846No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4240.2279300297814237No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCTACAGTTTGG4180.22470460483168656No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3950.21234047585769422No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGGCCAAGTTTGA3930.21126533420778187No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTAGACAAGTTCGA3870.20803990925804475No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3760.20212663018352667No Hit
CTACAGCAACCTTGTTACGACTTTTGCTTCCTCTAGCTGAGAAATATTGC3740.20105148853361432No Hit
GAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT3680.19782606358387717No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT3660.19675092193396482No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3570.1919127845093591No Hit
GAATCAGAATAAATGCTGATATAAAATTGGATCTCCTCCTCCTCTAGCAT3470.18653707625979724No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC3170.17040995151111157No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3150.16933480986119923No Hit
AAATCAGAATAAATGCTGGTAGAGAATAGGGTCTCCACCTCCAGCAGGGT3130.16825966821128682No Hit
AAATCAAAATAAATGTTGGTATAAAATTGGATCACCTCCACCTGCGGGAT3070.16503424326154972No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG3030.16288395996172494No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2910.1564331100622507No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA2900.15589553923729452No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2900.15589553923729452No Hit
AAATCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT2870.15428282676242597No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT2820.15159497263764501No Hit
AAATCAGAATAAATGCTGATATAAAATTGGATCTCCTCCTCCTCTAGCAT2740.1472944060379955No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAT2600.13976841448860888No Hit
GAATCAAAAAAGATGTTGATATAAGATAGGATCCCCACCCCCACCAACAA2510.13493027706400318No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC2490.1338551354140908No Hit
GAACCAGAATAGATGTTGGTATAAGACAGGGTCTCCACCCCCAACAGGGT2430.13062971046435368No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2360.12686671468966038No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA2350.12632914386470417No Hit
GAACCAAAATAAATGTTGGTATAAAATTGGATCACCTCCACCTGCGGGAT2250.1209534356151423No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATAAAACAGTTTGG2230.1198782939652299No Hit
TACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGGA2190.11772801066540517No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA2190.11772801066540517No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC2180.11719043984044898No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT2160.11611529819053659No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGAAATAGCAGTTTGA2140.11504015654062423No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA2140.11504015654062423No Hit
GAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT2110.11342744406575567No Hit
GAACCAGAATAAATGCTGGTAGAGAATAGGGTCTCCACCTCCAGCAGGGT2110.11342744406575567No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGATTTAC2020.10858930664114996No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1920.10321359839158809No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCACCTCCTGCTGGAT1910.1026760275666319No Hit
GAACCAGAATAAATGCTGATATAAAATTGGATCTCCTCCTCCTCTAGCAT1870.10052574426680716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)