FastQCFastQC Report
Tue 22 Apr 2025
Site5L_S109_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5L_S109_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186022
Total Bases35.1 Mbp
Sequences flagged as poor quality0
Sequence length52-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT2966815.948651234800185No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT158558.52318542968036No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT108565.835868875724377No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT71703.854382814935868No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA58823.161991592392298No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT45922.4685252281988155No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT41122.210491232219845No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTG24351.3089849587683178No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG18931.0176215716420638No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGGCCTGTCTTGGTT17760.9547257851221899No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG17710.9520379309974089No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG16460.8848415778778854No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG14850.7982926750599392No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG14650.7875412585608154No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT13150.7069056348173872No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG12590.6768016686198406No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG11990.6445474191224694No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG11420.6139058820999667No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG11220.6031544656008428No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG10460.5622990829041726No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG9780.5257442668071518No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG8820.4741374676113578No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG7990.42951908913999415No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG7420.3988775521174915No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT6990.37576200664437537No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG6960.3741492941695068No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG6820.36662330262012016No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC6820.36662330262012016No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT6740.3623227360204707No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG5980.3214673533238004No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG5880.31609164507423854No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG5360.28813796217651677No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG5270.28329982475191107No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG5230.2811495414520863No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4790.25749642515401405No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTTCGGAGGCT4720.25373342937932075No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG4720.25373342937932075No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACCGACGCATTTCAGTTT4620.24835772112975887No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAGCTCCGTGGCTT4470.24029415875541604No Hit
CTACCGATTGAGTGTTTTAGTGAGGACCTCGGATTGCTTGCCTGGCGGTT4290.23061788390620463No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG4180.22470460483168656No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT4180.22470460483168656No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATCGGCGCTCTGGAGAAT4140.22255432153186183No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT4090.21986646740708085No Hit
CTACCGATTGGATGGTTTAGTGAGGTTATTGGATTGGCGTTGGTGAGGTG4020.21610347163238758No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3980.2139531883325628No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT3960.21287804668265042No Hit
CTACCGATTGAGTGGATTAGTGAGATCCCCGGATCGGCGCCGACATGATG3920.2107277633828257No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG3850.20696476760813237No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3830.20588962595822No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3770.20266420100848287No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3750.20158905935857052No Hit
TCCTCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG3580.1924503553343153No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3570.1919127845093591No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG3470.18653707625979724No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3410.18331165131006008No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG3320.1784735138854544No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC3310.1779359430604982No Hit
TCCCCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG3310.1779359430604982No Hit
CTACCGATTGAGTGGTCCGGTGAACCCTCGGGATCGCATGCAATTTCTTT3290.17686080141058586No Hit
TCCTCCTCTCTCTGGAAATTTAGCTCATGCTGGAGGATCTGTCGACTTGG3290.17686080141058586No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3260.17524808893571728No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG3100.16664695573641827No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG3080.1655718140865059No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3060.16449667243659352No Hit
TCCTCCTCTTTCTAGAAATATTGCGCATGCTGGAAGGTCTGTAGATTTTG2980.16019610583694402No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT2970.15965853501198785No Hit
TCCCCCTCTTTCTAGAAATATTGCGCATGCTGGAAGGTCTGTAGATTTTG2950.15858339336207544No Hit
CCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2850.15320768511251356No Hit
CCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2800.15051983098773264No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC2790.14998226016277644No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA2750.1478319768629517No Hit
TCCCCCTCTCTCTGGAAATTTAGCTCATGCTGGAGGATCTGTCGACTTGG2690.1446065519132146No Hit
CCCTCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG2600.13976841448860888No Hit
CCCTCCTCTCTCTGGAAATTTAGCTCATGCTGGAGGATCTGTCGACTTGG2560.13761813118878413No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGACAGGTTTGGTT2380.12794185633957275No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACCGGACGCTGGCTTCGG2290.12310371891496705No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCGT2270.12202857726505466No Hit
CCCCCCTTTATCTAGTGGTACTGCACATTCAGGTGGAGCTGTAGATTTAG2270.12202857726505466No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT2250.1209534356151423No Hit
CAACCGATTGAATGGTTTGGTGAAATATTGGGATTTTACTTGGTGGGGCT2210.11880315231531755No Hit
CTACCGATTGAATGAATTAGTGAGGCCTTTGGATTTAGAAGCGCTTTTTC2150.11557772736558042No Hit
CTACCGATTGAATGATCCGATGAATATCGTGGACTGAGGGTGTAAGCGGG2150.11557772736558042No Hit
CTACCGATCGGATTTATTAGTGAGAACTAAGGACAAATCTTTTTTGCCTT2150.11557772736558042No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT2110.11342744406575567No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG2100.11288987324079948No Hit
CCCCCCTCTCTCTGGAAATTTAGCTCATGCTGGAGGATCTGTCGACTTGG2060.11073958994097471No Hit
CCCTCCTCTTTCTAGAAATATTGCGCATGCTGGAAGGTCTGTAGATTTTG2050.11020201911601854No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG2030.10912687746610616No Hit
CCCCCCTCTTTCTAGAAATATTGCGCATGCTGGAAGGTCTGTAGATTTTG1920.10321359839158809No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGCTTGGT1870.10052574426680716No Hit
CTACCGATCGAGTGTTTAGGTGAAATTGATAGACGTAAGGAAGTCAATTA1870.10052574426680716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)