FastQCFastQC Report
Tue 22 Apr 2025
Site5J_S107_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite5J_S107_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Total Bases10.8 kbp
Sequences flagged as poor quality0
Sequence length112-281
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG2434.285714285714285No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG811.428571428571429No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCTACAGTTCGG57.142857142857142No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC57.142857142857142No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG57.142857142857142No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC22.857142857142857No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAACCACTACAGTTTCG11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG11.4285714285714286No Hit
CTACGGAAACCTTGTTTCGACTTTTACTTCCTCTAAATGCAACAGATTGG11.4285714285714286No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAGGACAGGCTCGA11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA11.4285714285714286No Hit
GAATCAAAACAAATGCTGATAAAGAACAGGATCTCCACCACCAGCAGGAT11.4285714285714286No Hit
CTACGGAAACCCTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG11.4285714285714286No Hit
CTACGGAAGCCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGCCAAGATTTGA11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAGCAGTCTGG11.4285714285714286No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG11.4285714285714286No Hit
AAACCAAAATAAGTGCTGATATAAAATAGGGTCTCCTCCCCCCCCTACAT11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG11.4285714285714286No Hit
CCTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTA11.4285714285714286No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTTTACTTACTCTAAAAGCAACAGTTTGG11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAA11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA11.4285714285714286No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATTGCAAGGTTTAC11.4285714285714286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)