FastQCFastQC Report
Tue 22 Apr 2025
Site1y_S120_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1y_S120_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156819
Total Bases29.8 Mbp
Sequences flagged as poor quality0
Sequence length33-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG1595110.171599104700322No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG125828.023262487326154No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG98856.303445373328486No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT48203.073607152194568No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA38852.4773783788954145No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC33892.1610901740222803No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA31432.004221427250524No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT31211.9901925149376034No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC28161.7957007760539219No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT24491.5616730115610993No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA22971.4647459810354613No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT22891.4596445583762172No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG22701.4475286795605125No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG18731.1943705800955242No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT18181.159298299313221No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT17321.1044580057263471No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG17041.0866030264189925No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA16391.0451539673126344No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT13630.8691548855687129No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT12370.7888074786856184No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA12170.7760539220375082No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG11680.7448077082496382No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT11280.7193005949534177No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG10290.6561704895452719No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT9160.5841128944834492No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC8270.5273595673993585No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT8060.5139683329188427No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA7840.49993942060592145No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC7090.45211358317550804No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA7050.449562871845886No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT6330.4036500679126891No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT6030.3845197329405238No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA5670.36156333097392535No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5650.36028797530911433No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5530.3526358413202482No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4980.317563560537945No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTCAGGTTTAA4810.3067230373870513No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4550.290143413744508No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC4460.2844043132528584No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTCGG4330.2761145014315868No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4260.27165075660474813No Hit
AAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT4220.2691000452751261No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA4210.2684623674427206No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA4180.26654933394550406No Hit
CTACGGAAACCTTGTTACGACTTCACCATTCTCTAAGTAGTCTGGTTTAA4150.2646363004482875No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA4140.263998622615882No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4040.2576218442918269No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC3990.2544334551297993No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3960.2525204216325828No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCAACAGTTTGG3770.24040454281687804No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3770.24040454281687804No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGGTAATTCGC3700.23594079799003947No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3660.2333900866604174No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA3390.21617278518546862No Hit
GAATCAAAATAAATGCTGGTATAAGATTGGGTCACCTCCCCCTGCAGGGT3310.2110713625262245No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3310.2110713625262245No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG3270.20852065119660249No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3180.20278155070495282No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3110.19831780587811426No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3080.1964047723808977No Hit
GAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2960.18875263839203157No Hit
AAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2920.18620192706240954No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA2900.1849265713975985No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT2820.17982514873835442No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2760.17599908174392134No Hit
GAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2750.17536140391151583No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA2690.17153533691708275No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG2680.17089765908467724No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2610.16643391425783866No Hit
AAATCAAAATAAATGCTGGTATAAGATTGGGTCACCTCCCCCTGCAGGGT2600.16579623642543315No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2490.1587817802689725No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACACTTAAGCTCGA2340.14921661278288983No Hit
GAACCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2300.14666590145326777No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT2250.14347751229124023No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG2230.1422021566264292No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG2220.1415644787940237No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT2200.14028912312921266No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG2170.13837608963199613No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA2110.13455002263756305No Hit
CTACGGAAACCTTGTTACGACTTCACCATCCTCTAAATAGTTTGGTTTAA2100.13391234480515754No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT1980.1262602108162914No Hit
AAACCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1980.1262602108162914No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT1930.12307182165426384No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGCGATCAAGTTTGA1900.1211587881570473No Hit
CTACGGTTACCTTGTTACGACTTCACCCCAGTTACTGAATCCACTGTGGA1900.1211587881570473No Hit
CTACGGAAACCTTGTTACGACTTTTACTATCTCTAAATGAACAAGTTTGA1890.12052111032464177No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT1880.11988343249223626No Hit
GAATCAAAAAATATGCTGAAATAGGACAGGGTCTCCGCCTCCTGAGGGAT1820.1160573654978032No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG1790.11414433200058667No Hit
AAACCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1790.11414433200058667No Hit
GAACCAAAATAAATGCTGGTATAAGATTGGGTCACCTCCCCCTGCAGGGT1770.11286897633577564No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC1760.11223129850337012No Hit
AAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1690.10776755367653156No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA1670.10649219801172052No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC1670.10649219801172052No Hit
GAATCAAAACAAATGCATAAATAAAACTGGATCGCCCCCGCCCACAGGGT1670.10649219801172052No Hit
CTACGAAAACCTTGTTACGACTTTTACTTCCTAGAAGTGATCAAGTTCGT1670.10649219801172052No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATAAGGTTTAG1620.10330380884969297No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT1570.10011541968766541No Hit
AAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT1570.10011541968766541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)