FastQCFastQC Report
Tue 22 Apr 2025
Site1w_S118_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1w_S118_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198881
Total Bases34.3 Mbp
Sequences flagged as poor quality0
Sequence length37-281
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG2138010.750147072872723No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC107785.419321101563247No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG105465.302668429865095No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG95234.788290485265058No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC78103.9269714050110363No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA59012.967100929701681No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA57632.897712702570884No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG49532.4904339781075113No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT36431.8317486336050202No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG35371.7784504301567268No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA32941.6562668128177152No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG24411.2273671190309783No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA23731.193175818705658No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC23521.1826167406640153No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA22351.1237875915748614No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT21571.0845681588487588No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTCGG19190.9648986077101382No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC19070.9588648488291993No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC18550.932718560345131No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA16070.8080208768057281No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT15200.7642761249189214No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA13980.7029329096293764No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG12810.6441037605402224No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA12370.6219799779767801No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT11910.5988505689331811No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC10630.5344904742031668No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT10220.513875131359959No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT8710.43795033210814505No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT8650.4349334526676757No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA8600.4324193864672844No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC8050.40476465826298136No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT7960.4002393391022772No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT7500.37710993005867827No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG6580.3308511119714804No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT6540.32883985901116747No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT6490.3263257928107763No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA6300.31677234124928977No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG5790.29112886600529964No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5770.2901232395251432No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT5700.2866035468445955No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG5680.28559792036443904No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT5310.26699383048154424No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4930.2478869273585712No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA4820.2423559817177106No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG4760.23933910227724114No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACTGAGTTTGA4730.23783066255700647No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT4660.23431096987645877No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4400.22123782563442462No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG4360.21922657267411166No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT4120.20715905491223396No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT4100.20615342843207746No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4060.2041421754717645No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3980.2001196695511386No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3920.1971027901106692No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGCTCAC3920.1971027901106692No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT3850.19358309743012153No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTAAC3710.1865437120690262No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3630.1825212061484003No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAG3610.18151557966824383No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATGTGGTTTAC3610.18151557966824383No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3570.17950432670793087No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATGTGGTTTGC3530.1774930737476179No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT3520.1769902605075397No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATATGGTTTAA3430.17246494134683554No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3410.17145931486667906No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATAAGGTTCAG3350.16844243542620962No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGGTTTGG3350.16844243542620962No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAG3330.16743680894605317No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA3220.16190586330519255No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTGG3170.15939179710480136No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT3160.15888898386472314No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA3160.15888898386472314No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATGTGGTTTAC3120.15687773090441018No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTACGATTTAT3120.15687773090441018No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA3080.15486647794409722No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3070.15436366470401897No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2970.1493355323032366No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2910.14631865286276718No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2890.1453130263826107No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG2860.143804586662376No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2840.14279896018221952No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATATGGTTAAC2820.14179333370206304No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG2810.14129052046198481No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG2680.1347539483409677No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC2640.13274269538065478No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATGTGGTTTAC2600.13073144242034182No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2570.1292230027001071No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2560.12872018946002886No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC2530.12721174973979416No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAA2490.1252004967794812No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTGC2420.12168080409893355No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC2360.1186639246584641No Hit
GAACCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2350.11816111141838587No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTAAGGTTCAC2350.11816111141838587No Hit
AAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2330.11715548493822939No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGCATCAGGATTTAA2270.11413860549775996No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTTAA2240.11263016577752526No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA2230.11212735253744702No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2150.1081048466168211No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT2090.10508796717635167No Hit
CTACGGAAACCTTGTTACGACTTCTGCTTCCTCTAAGTGATAAGGCTTGC2070.10408234069619522No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAC2060.10357952745611698No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2030.10207108773588225No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2010.10106546125572578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)