FastQCFastQC Report
Tue 22 Apr 2025
Site1v_S117_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1v_S117_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186257
Total Bases31.7 Mbp
Sequences flagged as poor quality0
Sequence length43-281
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT1975210.604702105155779No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT127826.862560870195483No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT92174.948538846862132No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT68833.6954315810949385No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA32591.7497328959448504No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG32491.7443639702131999No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT29391.577927272532039No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG28781.545176825568972No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGCT26441.4195439634483535No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGTT21081.1317695442318947No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG19041.0222434593062273No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG16660.894463026892949No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT14270.7661457019065055No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA12230.6566196169808384No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG11520.6185002442861208No Hit
CTACCGATTGAGTGATCTGGTGAACCTTCTGGACTGCGACCGTCCTCGAG10860.5830653344572284No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA10800.5798439790182383No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG10670.5728643755670928No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAGCTCCGGAGGTT10560.5669585572622774No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT10490.5632003092501221No Hit
TCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG10330.5546100280794816No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT9990.5363556805918703No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG9050.485887778714357No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG9050.485887778714357No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG8940.47998196040954166No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG8880.47676060497055145No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA8840.47461303467789134No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT8560.4595800426292703No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA8210.44078880256849406No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG8100.4348829842636787No Hit
TCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG8050.4321985213978535No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC7530.4042801075932716No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG7350.394616041276301No Hit
TCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG7220.38763643782515556No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA7150.3838781898130003No Hit
TCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG6990.3752879086423598No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG6860.36830830519121427No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG6800.36508694975222405No Hit
CCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG6720.3607918091669038No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGGCCTTGGGAGGAT6350.3409267839597975No Hit
CCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG6280.33716853594764223No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG6190.33233650278915694No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT6110.3280413622038366No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC5520.29636470038709956No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG5310.2850899563506338No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT5270.28294238605797367No Hit
CCCCCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG5250.28186860091164356No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG5200.27918413804581843No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4880.26200357570453725No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT4760.2555608648265568No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG4730.25395018710706174No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4560.24482301336325615No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4520.24267544307059602No Hit
CCCTCCTCTCTCTAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG4370.23462205447312046No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT4290.23032691388780016No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG4250.22817934359514008No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC4230.22710555844881No Hit
TCCCCCTCTCTCTAGTAATATCGCGCACGCGGGAAGTTCTGTGGACTTTG4220.22656866587564495No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT4180.2244210955829848No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG3790.20348228522954842No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG3790.20348228522954842No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA3760.2018716075100533No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3690.19811335949789805No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3520.18898618575409246No Hit
CTACCGATTGAACGTTTTAGTGAGGTACTTGGATTGGACCTTCGGAGATG3520.18898618575409246No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3460.18576483031510224No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCCGGCATGGTG3420.1836172600224421No Hit
CTACCGATTGAATGGTTCAGTGAGATAATCGGATAGGTAACTCGGTAAGC3410.18308036744927705No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACGTCGAGAGGTT3330.1787852268639568No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG3320.17824833429079176No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC3310.17771144171762673No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3260.17502697885180155No Hit
TCCTCCTCTCTCTAGTAATATCGCGCACGCGGGAAGTTCTGTGGACTTTG3130.1680473754006561No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT2990.1605308793763456No Hit
CCCCCCTCTCTCTAGTAATATCGCGCACGCGGGAAGTTCTGTGGACTTTG2980.15999398680318055No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG2910.1562357387910253No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2860.15355127592520013No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC2850.1530143833520351No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG2730.14657167247405467No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG2710.1454978873277246No Hit
TCCTCCTCTCTCCAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG2700.14496099475455956No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2580.13851828387657913No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT2570.1379813913034141No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG2570.1379813913034141No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG2520.13529692843758892No Hit
TCCCCCTCTCTCCAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG2490.1336862507180938No Hit
TCCTCCGAGCCCACGAGACTAACAGGTATCTCGTATGCCGTCTTCTGCTT2490.1336862507180938TruSeq Adapter, Index 2 (96% over 27bp)
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC2450.13153868042543368No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT2430.1304648952791036No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2420.12992800270593857No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG2180.11704258094997771No Hit
TCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG2110.11328433293782247No Hit
TCCCCCGAGCCCACGAGACTGATTCTTCGGCCACCCCGAAGTCTACTGTC2100.11274744036465743No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2030.10898919235250218No Hit
CTACCGATTGAATGATCCGGTGAGGCCCCCGGACTGGGGCGACGCATTCG1960.10523094434034692No Hit
TCCTCCGAGCCCACGAGACTGATTTTTCGGCCACCCCGAAGTCTACTGTC1940.10415715919401689No Hit
CCCTCCGAGCCCACGAGACTAACAGGTATCTCGTATGCCGTCTTCTGCTT1940.10415715919401689TruSeq Adapter, Index 2 (96% over 27bp)
TCCTCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG1890.1014726963281917No Hit
CCCTCCTCTCTCCAGTAATATCGCACACGCGGGAAGTTCTGTAGACTTTG1880.10093580375502666No Hit
TCCCCCATTAGCAAGTATTGCATTCCACTCAGGAGGAGCAGTTGATTGTG1870.10039891118186163No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)