FastQCFastQC Report
Tue 22 Apr 2025
Site1t_S113_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1t_S113_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198266
Total Bases37.4 Mbp
Sequences flagged as poor quality0
Sequence length52-281
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT2443512.324352132993049No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT79203.9946334722040087No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT63593.2073073547658195No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT58362.943520321184671No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG57432.8966136402610636No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG36591.845500489241726No Hit
CTACCGATTGAATGGTTTAGTGAGGTCTTTGGATCGAGATTAATGAATTG35421.7864888584023484No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT26591.341127576084654No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG23291.1746845147428202No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGCT20221.0198420304035993No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG19540.9855446723089183No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG16740.8443202566249383No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG15950.8044747964855296No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGTG15870.8004398131802729No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG15000.7565593697356077No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG14760.744454419819838No Hit
CTACCGATTGAGTGGTTTAGTGAGGCTCACGGACCGCTGGCGGCACCCGG14410.7268013678593405No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTGCGAGGGTT14320.7222620116409268No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT13800.6960346201567591No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA13590.6854427889804605No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC13220.666780991193649No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGGTAGGCGGAT12960.6536672954515651No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGAATTAGGTTAGTT12690.6400492267963241No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT12570.6339967518384393No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG12200.6153349540516276No Hit
TCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG11310.5704457647806482No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG11180.5638889169096063No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG11130.5613670523438209No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT11020.5558189502990931No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG10690.5391746441649098No Hit
TCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG9680.4882329799360455No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT9480.47814552167290403No Hit
TCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG8830.4453612823176944No Hit
TCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG8540.43073446783613933No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG8430.42518636579141156No Hit
CTACCGATTGAGTGGATTAGTGAGGACATCGGATTGAGAAATTGTTGGTG7570.3818102952599034No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCCGGCATGGTG7520.379288430694118No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG7420.3742447015625473No Hit
CCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG7310.3686965995178195No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT7030.3545741579494215No Hit
CCCTCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG7010.3535654121231073No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA6870.34650419133890836No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG6820.34398232677312296No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG6750.34045171638102345No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG6730.33944297055470934No Hit
CCCCCCTCTTTCAAGAAATATTGCCCATGCTGGTGGCTCTGTGGACTTTG6660.33591236016260984No Hit
CCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG6610.3333904955968245No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG6230.31422432489685576No Hit
CTACCGATTGAATGGCCCGGTGAAGACCTGGGACTACTGTCGTGGATTTA5930.29909313750214356No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCGGTGCCTTA5550.2799269668021749No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT5540.2794225938890178No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC5450.27488323767060413No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG5310.26782201688640517No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4980.25117771075222173No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGATTGGACGTCGAGAGGTT4890.2466383545338081No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4810.24260337122855155No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG4720.2380640150101379No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT4580.23100279422593892No Hit
TCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG4570.23049842131278184No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG4420.22293282761542574No Hit
CTACCGATTGAACGGCTTAGTGAGTTGTAGGGACCGGAGTCTGCGCGGGG4310.21738472557069793No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG4300.21688035265754085No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG4290.21637597974438377No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT4170.21032350478649894No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4050.20427102982861406No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG3970.2002360465233575No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT3890.19620106321810096No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG3790.1911573340865302No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3720.1876267236944307No Hit
TCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG3640.18359174038917414No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT3620.18258299456285998No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3610.18207862164970293No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA3400.1714867904734044No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3400.1714867904734044No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3370.16997367173393318No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC3290.16593868842867662No Hit
CTACCGATTGAATGGTTTAGTGAGACCCTCGGACTGGAGTCCGTCTGCCT3240.16341682386289128No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA3220.16240807803657714No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA3220.16240807803657714No Hit
CTACCGATTGAACGTGCTGGTGAAGAGTTTGGATTGGAGTTTTGGCTAGG3210.16190370512342006No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG3200.16139933221026298No Hit
CTACCGATTGAATGGTTCAGTGAGATAATCGGATAGGTAACTCGGTAAGC3170.15988621347079177No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG3170.15988621347079177No Hit
CCCTCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG3110.15685997599184934No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT3070.15484248433922104No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG3070.15484248433922104No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3000.15131187394712156No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG2890.14576377190239376No Hit
CTACCGATTGAACGGCTTAGTGAAATGTGAGGACCGAGGGGTGTGCAGGG2830.14273753442345133No Hit
CCCCCCTCTTGCGGGAAATGTGTTTCACTCCGGGCCTGCGGTGGACATGG2750.13870255111819474No Hit
CTACCGATTGAACGTTTTAGTGAGGCATTTGGACTGGGCCTTTGGAGGTT2680.13517194072609526No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCCCGCCC2650.13365882198662404No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2590.1306325845076816No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT2570.12962383868136745No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG2560.1291194657682104No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG2530.1276063470287392No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT2360.11903200750506895No Hit
TCCTCCTCTAAGAACCAATATCGGACATTCAGGAATATCTGTAGATTTAA2360.11903200750506895No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGGATTCAATGCCTTT2340.11802326167875482No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA2320.11701451585244066No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACCGCGACGCCAGAGCGC2250.11348390546034115No Hit
TCCTCCTTTATCCTCTAATATTGCCCAGGAAGGTGCAAGAATGGACTTAG2230.11247515963402702No Hit
CTACCGATTGAATAGTTTAGTGAGGCACTTGGACTGGAACTCTCGAGGTT2130.1074314305024563No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTTCGGAGGCT2130.1074314305024563No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT2110.10642268467614216No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGTCTCGACATGGTG2110.10642268467614216No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG2110.10642268467614216No Hit
CTACCGATTGAACGGTTTAGTGAGACCCTCGGATTGGTCCCGGTACGGGG2080.10490956593667093No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGTCAGTTTGCTT2070.10440519302351388No Hit
TCCTCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2060.1039008201103568No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGGCGCCATCGTGGCT2040.10289207428404265No Hit
TCCCCCTCTTTCTAGAAACATCGCCCACGCAAGACCAGCTGTTGACATGG2020.10188332845772852No Hit
CTACCGATTGGATGGTTTAGTGAGACCCACGGATTGGTCCCGGCATGGTG2000.10087458263141436No Hit
TCCCCCTTTATCCTCTAATATTGCCCAGGAAGGTGCAAGAATGGACTTAG1990.10037020971825729No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG1990.10037020971825729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)