FastQCFastQC Report
Tue 22 Apr 2025
Site1s_S112_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1s_S112_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200028
Total Bases38.3 Mbp
Sequences flagged as poor quality0
Sequence length41-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG105955.296758453816466No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG102515.124782530445737No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC91594.578858959745635No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC79663.982442458055872No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG63733.1860539524466573No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG62823.1405603215549824No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG43502.1746955426240326No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA39271.963225148479213No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT39051.9522266882636432No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG36811.8402423660687501No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA34011.7002619633251344No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG29531.476293318935349No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA29051.4522966784650149No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC26501.3248145259663648No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA26171.30831683564301No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA22151.1073449717039614No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT19800.9898614194012839No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT18790.9393684884116225No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC17350.86737856700062No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT15480.7738916551682765No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT14670.7333973243745876No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC13730.6864039034535165No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT12960.6479092926990221No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC12790.6394104825324455No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT12640.631911532385466No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT12380.6189133521307016No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA11980.5989161517387566No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT11260.5629211910332553No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT10920.545923570700102No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT9710.48543203951446795No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA9710.48543203951446795No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA9400.46993420921071055No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT9130.45643609894614756No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT8890.44443777871098045No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT8880.4439378487011818No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT8240.4119423280740696No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT7930.39644449777031215No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG7670.38344631751554786No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA7220.3609494670746095No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT7010.3504509368688383No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6800.33995240666306714No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT6720.33595296658467816No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC6600.3299538064670946No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA6570.3284540164376987No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6270.31345611614373986No Hit
AAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT6080.30395744595756596No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTCGA6040.30195772591837144No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6000.2999580058791769No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT5840.29195912572239885No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA5410.27046213530105784No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT5120.25596416501689767No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT5050.2524646549483073No Hit
GAACCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT4980.24896514487971685No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4680.23396724458575802No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT4430.22146899434079226No Hit
GAATCAAAAAAGGTGTTGGAATAACACAGGGTCTCCTCCACCAGCTGGGT4100.20497130401743754No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATATGGTTTAA3900.194972703821465No Hit
GAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT3880.19397284380186774No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGGGCGGTTTGG3870.19347291379206913No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG3850.19247305377247187No Hit
AAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT3780.18897354370388147No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACTGAGTTTGA3780.18897354370388147No Hit
AAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT3760.18797368368428422No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTAAGGTTCAC3730.1864738936548883No Hit
GAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT3520.17597536344911713No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3510.17547543343931848No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA3420.17097606335113083No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGCTCAC3370.1684764133021377No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAG3350.16747655328254044No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC3340.16697662327274182No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3270.16347711320415143No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATGTGGTTTAC3260.16297718319435278No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGCAATAAGGTTAAC3180.15897774311596377No Hit
AAACCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT3170.15847781310616513No Hit
GAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT3150.15747795308656787No Hit
GAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT3150.15747795308656787No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATGTGGTTTGC3120.155978163057172No Hit
GAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2990.14947907292978982No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAG2990.14947907292978982No Hit
AAATCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2930.14647949287099807No Hit
GAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2920.14597956286119942No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATATGGTTAAC2810.14048033275341454No Hit
GAATCAAAATAAGTGCTGATAAAGAACAGGGTCCCCTCCTCCAGCAGGGT2740.13698082268482412No Hit
AAATCAAAAAAGGTGTTGGAATAACACAGGGTCTCCTCCACCAGCTGGGT2700.13498110264562962No Hit
GAATCAAAAGAGGTGTTGGTAGAGAATTGGGTCTCCTCCTCCCGCCGGGT2660.1329813826064351No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTACGATTTAT2560.12798208250844884No Hit
GAACCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT2540.12698222248885158No Hit
AAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2520.12598236246925432No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATTGGATTTAC2440.12198292239086529No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG2380.11898334233207351No Hit
GAATCAGAAGAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2370.11848341232227488No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA2330.11648369228308038No Hit
AAATCAGAAAAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2320.11598376227328175No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG2310.11548383226348312No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGGTAAGGTTTGA2260.11298418221448998No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGGTTTGG2260.11298418221448998No Hit
GAATCAGAATAAATGTTGATATAAAACAGGATCACCACCACCTGCTGGAT2200.10998460215569819No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA2200.10998460215569819No Hit
GAACCAGAAAAGATGCTGGTAAAGCACTGGATCTCCACCTCCTGCTGGAT2190.10948467214589956No Hit
GAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT2180.10898474213610093No Hit
GAACCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT2170.1084848121263023No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTGG2140.10698502209690643No Hit
AAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT2130.1064850920871078No Hit
AAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT2110.10548523206751054No Hit
AAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT2100.10498530205771191No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATGTGGTTTAC2080.10398544203811466No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2070.10348551202831603No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGCATCAGGATTTAA2070.10348551202831603No Hit
AAATCAGAAGAGATGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2040.10198572199892014No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTGC2020.10098586197932288No Hit
GAACCAAAAAAGGTGTTGGAATAACACAGGGTCTCCTCCACCAGCTGGGT2010.10048593196952425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)