FastQCFastQC Report
Tue 22 Apr 2025
Site1p_S105_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1p_S105_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059779
Total Bases181.3 Mbp
Sequences flagged as poor quality0
Sequence length17-281
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG717406.769335870969324No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG582135.492937678515992No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCACCAGTTCGA487314.598222837025456No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG355493.3543786015763666No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG337393.1835882764236696No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG273412.5798775027623684No Hit
GAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT186541.7601783013250876No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA174081.6426066189271535No Hit
AAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT137451.2969685189081874No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA107231.0118147274101488No Hit
GAACCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT103620.9777510216752738No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC92650.8742388743313465No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT88200.8322489877606557No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG84930.8013934980783729No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA83180.78488062133709No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC79810.7530815386981625No Hit
AAACCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT73550.6940126196122022No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG63900.6029558992959854No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT62160.586537381850367No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA55290.5217125457288737No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT50660.4780241918362225No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATCATGAGGTTTGG48330.4560384759464001No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT48280.4555666794680778No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA48230.4550948829897554No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGAGAGCTTAATTAGA46440.4382045690658146No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGG45230.4267870942904134No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT44980.42442811189880153No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG44100.41612449388032785No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA41920.39555416742547267No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT40400.3812115544844727No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT40160.37894693138852537No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA36630.3456381000189662No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT31320.2955333140211308No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT30790.29053227135091375No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA29650.2797753116451637No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT29410.2775106885492164No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28370.26769732180011113No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT28330.26731988461745326No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCAACAGTTTGG26550.250523929989177No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGCGATCAAGTTTGA26290.24807058830190065No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT25500.2406162039444073No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT22040.2079678876444995No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT21570.20353300074826922No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT21470.2025894077916245No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA21180.19985298821735475No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC20980.19796580230406527No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT20560.1940027118861574No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG19720.18607653105034164No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATGTGGTTTAC18670.17616880500557192No Hit
GAATCAGAATAAGTGTTGATAAAGAATAGGATCACCTCCTCCTGTAGGGT18480.17437597838794694No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT17610.16616671966513774No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG17110.16144875488191407No Hit
CTACGAAAACCTTGTTACGACTTTTACTTCCTAGAAGTGATCAAGTTCGT16510.15578719714204567No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT16190.1527676996807825No Hit
GAATCAGAATAGATGTTGATATAAAATAGGGTCACCACCCCCGGCAGGGT15820.149276405741197No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA15690.14804973489755882No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT14960.14116150631405228No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT14960.14116150631405228No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC13870.13087634308662466No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG13540.12776248632969703No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC13400.1264414561903944No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA13250.12502606675542732No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGCACCAGTTCGA13250.12502606675542732No Hit
AAATCAGAATAAGTGTTGATAAAGAATAGGATCACCTCCTCCTGTAGGGT13020.12285580295514442No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA12960.12228964718115758No Hit
GAATCAGAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT12640.11927014971989444No Hit
GAATCAGGCTCGGAGGATAAACAGTTCAACCAGTACCCTGTCTCTTATAC12640.11927014971989444No Hit
GAATCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT12570.11860963465024311No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT12200.1151183407106576No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA12160.1147409035279997No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTCGG12060.11379731057135498No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC11850.11181576536240102No Hit
AAATCAGAATAGATGTTGATATAAAATAGGGTCACCACCCCCGGCAGGGT11810.11143832817974314No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA11410.1076639563531642No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTTAA11130.10502189607455893No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG11030.10407830311791422No Hit
GAATCAGGCTCGGGGGATAAACAGTTCAACCAGTACCCTGTCTCTTATAC10820.10209675790896026No Hit
GAATCAGGCTCGGAGGATAAACAGTTCAACCTGTACCCTGTCTCTTATAC10680.10077572776965764No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)