FastQCFastQC Report
Tue 22 Apr 2025
Site1o_S104_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1o_S104_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207227
Total Bases38.4 Mbp
Sequences flagged as poor quality0
Sequence length51-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT185298.941402423429379No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT93354.50472187504524No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT68173.289629247154087No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT33261.605003209041293No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG32681.577014578216159No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT32311.5591597620001254No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG29221.4100479184662231No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG25651.2377730701115202No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG23591.1383651744222518No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG21681.0461957177394838No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG21201.0230327129186834No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG20250.977189265877516No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA20230.9762241406766493No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG20080.9689857016701492No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT18370.8864674969960478No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG17530.8459322385596472No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG17290.8343507361492469No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT16860.8136005443306134No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGGCGCCATCGTGGCT16820.8116702939288799No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG16680.8049144175228131No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG14780.7132275234404784No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG14250.6876517056175113No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA14170.6837912048140445No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT13980.674622515405811No Hit
CTACCGATTGGATGGCTTAATGAGATCTCTGGATCTCTGCCGGCGCGGTC13760.6640061381962775No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG13650.6586979495915107No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG13450.649046697582844No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT12650.6104416895481767No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA12650.6104416895481767No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG12510.6036858131421099No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT12040.5810053709217429No Hit
CTACCGATTTCGAGTGATCCGGTGAACCATTCGGACCGGCGCCGCTTCGT10520.507655855655875No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG10240.49414410284374144No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA9790.4724287858242411No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG9240.4458878428004073No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGTGCCTCGA9240.4458878428004073No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG8680.4188643371761402No Hit
CTACCGATTGAACGGTTTAGTGAGACCCTCGGATTGGTCCCGGTACGGGG8600.41500383637267346No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA8470.40873052256704No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC7870.37977676654103953No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC7860.3792942039406062No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG7790.3759162657375728No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG7770.37495114053670614No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG7640.3686778267310727No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG7330.3537183861176391No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG7180.346479947111139No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT6310.30449700087343834No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT6300.30401443827300495No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC6290.3035318756725716No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTTCCTT6280.3030493130721383No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG6260.3020841878712716No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT6080.2933980610634715No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT6080.2933980610634715No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG5870.28326424645437126No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCCCGCCC5730.27650837004830453No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT5690.27457811964657114No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG5420.26154892943487096No Hit
CTACCGATTGAATGGTTTAGTGAGACCCTCGGATTTGTCCCAGCAGGGTG5400.26058380423400423No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG5380.25961867903313757No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG5320.2567233034305375No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC5160.24900230182360408No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTGCCTTT5110.24658948882143739No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGACGGTGCTCG5030.24272898801797066No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG4910.23693823681277057No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG4760.2296997978062704No Hit
CTACCGATCGGATGGTTTAGTGAGACCCTTGGACTGGTCCCGTCGAAGTG4730.22825211000497037No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG4630.22342648400063697No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGCT4540.2190834205967369No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTGGCTCCTT4540.2190834205967369No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG4510.21763573279543688No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT4500.21715317019500355No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT4310.20798448078677007No Hit
CTACCGATTTCGAGTGATTCGGTGAACCATTTGGACTGGAGGAGACCGAG4070.19640297837636989No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGCTGGCTCGGCGGCC3990.19254247757290313No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG3870.18675172636770304No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTTCCTT3830.18482147596596968No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTTTCCTT3800.18337378816466965No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG3800.18337378816466965No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC3780.18240866296380298No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGTATGCTGAG3710.1790307247607696No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT3670.17710047435903623No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCGCGTGGCTTCG3440.16600153454906938No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT3400.16407128414733602No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG3330.16069334594430262No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGATTTTGGCTTAGTTGCTT3320.1602107833438693No Hit
TCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG3290.15876309554256926No Hit
TCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG3260.15731540774126923No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA3130.1510420939356358No Hit
CTACCGATTGAGTGGTTTAGTGAGGCTCACGGACCGCTGGCGGCACCCGG3070.14814671833303575No Hit
TCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG3060.14766415573260241No Hit
TCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG3020.14573390533086905No Hit
TCCCCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG2920.14090827932653563No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC2880.13897802892480227No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT2830.13656521592263557No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGGATTCAATGCCTTT2810.13560009072176887No Hit
CTACCGATTGAATGTGTTGGTGAGGAGCTCGGATTGGCAGCAGTTGGTGG2790.1346349655209022No Hit
CTACCGATTGAACGTTTTAGTGAGGTGTTTGGACCTGGCCTGGAGGGGCT2780.13415240292046884No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT2740.13222215251873548No Hit
TCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2740.13222215251873548No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGCGCAGG2690.12980933951656878No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGAGCTCCGGAGGTT2660.12836165171526875No Hit
CCTCCGATAGAATGGCTAAGCGAGGCCGTTGGACTGGGCCTGCTTTCGAG2610.12594883871310206No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCGAGTTCCCTT2500.12064065010833531No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGACTGGACCTCTGAAGGTT2490.12015808750790197No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGCTAGGACTTG2490.12015808750790197No Hit
TCCTCCTTTATCAAGAAATATTGCTCACTCTGGGGCATCTGTAGATTTAT2430.11726271190530191No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCATTCCGTTGG2400.11581502410400188No Hit
CCCTCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2360.11388477370226852No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGCTTGGT2330.1124370859009685No Hit
CCCTCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG2320.11195452330053515No Hit
CTACCGATTGAATGGTCCGGTGAAAACTTCGGACTGTGGCAACGTTGCTT2300.11098939809966847No Hit
CCCTCCTCTTTCAAGTGCAACAGCTCATTCAGGAGGTTCCGTAGACTTAG2290.11050683549923514No Hit
TCCTCCGTTAAGGGGAAACCTGGCCCACAGTGGGAGGTCAGTTGATTTCG2230.10761145989663509No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGAGAGTAGCGTACCTTG2230.10761145989663509No Hit
TCCCCCTTTATCAAGAAATATTGCTCACTCTGGGGCATCTGTAGATTTAT2220.10712889729620174No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG2140.10326839649273502No Hit
CCCCCCATTGTCTAGTATTACAGCACACTCGGGTGGAGCTGTTGATTTAG2120.10230327129186834No Hit
CTACCGATTGAATGGTCCGGTGAAGACTCGAGACTGTGGTCTGGCTCCTT2090.10085558349056832No Hit
CCCCCCGCTAAGGTCCAACATCGCCCACAGAGGGATGTCGGTGGACTTTG2080.10037302089013497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)