FastQCFastQC Report
Tue 22 Apr 2025
Site1n_S103_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1n_S103_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240093
Total Bases43.2 Mbp
Sequences flagged as poor quality0
Sequence length28-281
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC124685.1929877172595615No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG113264.717338697921222No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG112734.69526391856489No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG103614.315411111527617No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA100924.203371193662456No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG82003.415343221168464No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG78303.261236270945009No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA68892.869304811052384No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC63192.631896806654088No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG53842.242464378386708No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA46681.9442466044407791No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG42571.7730629381114817No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC37631.5673093342996256No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGACCAAGTTTGG33551.397375183782951No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG22760.9479659965096858No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA21700.9038164377970204No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGAGAGCTTAATTAGA21650.9017339114426494No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT18360.7647036773250365No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT16860.7022278866939061No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA16330.6801531073375734No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA15770.656828812168618No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT15230.634337527541411No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATGTGGTTTAC13800.5747772738064001No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC12600.5247966413014957No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC12180.5073034199247791No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT11870.49439175652767886No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC11620.48397912475582383No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT11040.45982181904512004No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT10960.45648977687812636No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGCGATCAAGTTTGA10930.45524026106550375No Hit
GAATCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT9390.3910984493508765No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC9050.37693727014115364No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT8980.3740217332450342No Hit
GAATCAGAACAAGTGTTGGTATAATACAGGATCTCCCCCACCCCCTACAT8270.3444498590129658No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATGTGGTTTAC8150.3394517957624754No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT8110.3377857746789786No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT7970.33195470088673973No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG7780.3240411007401299No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT7770.3236245954692557No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG7680.3198760480313878No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT7330.3052983635507907No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT6820.2840565947362064No Hit
CTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAACGGGAGGGTTCAA6800.283223584194458No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTGA6730.2803080472983385No Hit
AAATCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT6520.27156143660998033No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA6440.2682293944429867No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT6400.2665633733594899No Hit
CTACGGATACCTTGTTACGACTTTTACGTCCTCTACCCCAGATAATTTGT6280.2615653101089994No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT6250.26031579429637686No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT6180.2574002574002574No Hit
CTACGAAAACCTTGTTACGACTTTTACTTCCTAGAAGTGATCAAGTTCGT6040.25156918360801855No Hit
AAATCAGAACAAGTGTTGGTATAATACAGGATCTCCCCCACCCCCTACAT6010.25031966779539594No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT5850.2436555834614087No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG5750.23949053075266666No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5560.2315769306060568No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATGTGGTTTAC5540.23074392006430844No Hit
GAACCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT5200.2165827408545855No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA5170.2153332250419629No Hit
GAACCAGAACAAGTGTTGGTATAATACAGGATCTCCCCCACCCCCTACAT4860.20242156164486264No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4820.2007555405613658No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA4800.19992253001961738No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC4760.1982565089361206No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAG4720.19659048785262379No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4580.19075941406038494No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT4440.18492834026814609No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4410.18367882445552347No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATCAAGTTTGG4330.18034678228852985No Hit
GAATCAGAATAGATGCTGGTAAAGAATAGGGTCTCCCCCGCCTCTTGGGT4200.17493221376716522No Hit
CTACGGAAACCTTGTTACGACTTCACCATCTTCTAGATAACGAGGTTAAA3940.16410307672443594No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3930.16368657145356175No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTCGA3740.1557729713069519No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3730.15535646603607767No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA3610.15035840278558724No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATTAAGCTCGA3580.14910888697296465No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3430.1428613079098516No Hit
AAACCAGAACAAGTGTTGGTATAATACAGGATCTCCCCCACCCCCTACAT3390.1411952868263548No Hit
AAACCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT3310.13786324465936114No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT3280.13661372884673856No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGTTACGATTTAT3230.13453120249236752No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATATGGTTTAA3160.1316156655962481No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAATCAAGTTTGA3150.13119916032537393No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA3140.1307826550544997No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATGTGGTTTAC2930.12203604436614145No Hit
AAATCAGAATAGATGCTGGTAAAGAATAGGGTCTCCCCCGCCTCTTGGGT2910.12120303382439304No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2730.1137059389486574No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT2730.1137059389486574No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTTGC2690.11203991786516058No Hit
GAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT2690.11203991786516058No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2670.11120690732341217No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT2650.11037389678166377No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC2600.10829137042729275No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGGTTTGG2530.10537583353117333No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG2520.10495932826029913No Hit
CTACAGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC2510.10454282298942494No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA2420.10079427555155711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)