FastQCFastQC Report
Tue 22 Apr 2025
Site1n_S103_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1n_S103_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240093
Total Bases43.2 Mbp
Sequences flagged as poor quality0
Sequence length35-281
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT110164.588222063950219No Hit
CTACCGATTGAACATTTTAGTGAGGTCCTCGGACTGTGAGCCAGGCGGGT105404.389965555014099No Hit
CTACCGATTGAATGATTCGGTGAAAATCTCGGACTTTGGCATGGAACTTT103444.308330521922755No Hit
CTACCGATTGAATGTTTTAGTGAGGTCCTCGGATTGTTGGTCTGGCGGTT83123.4619918115063744No Hit
CTACCGATTGAACGTTTTAGTGAGGTCCTCGGACTGTTTGCCTGGCGGAT80013.3324586722644973No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCTTCG57282.3857421915674344No Hit
CTACCGATTGAATGGTCCGGTGAGGCCTCGAGATCGTGGCAAGCTTTCTT54762.280782863307135No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGACCTCTGGAGGCT48612.0246321217195002No Hit
CTACCGATTGAACGTTTTAGTGAGGTATTTGGATTGGCTTAATTGAGGGT47971.9979757843835513No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCAGCTTCG38241.5927161558229521No Hit
CTACCGATGGATGATTTGGTGAGGTCGCCTAGACTGGCCGCTGGCTTCGG35151.464016027122823No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACCCTGCATGGTG33651.401540236491693No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCTTGGTCTTG27471.1441399790914355No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGGCGCCATCGTGGCT24771.0316835559554005No Hit
CTACCGATTGAATGTCATAGTGAGGTCCTCGGACTGTTTGGTTGGCGGAT23390.9742058285747606No Hit
CTACCGATTGGATGGCTTAATGAGATCTCTGGATCTCTGCCGGCGCGGTC23010.9583786282815409No Hit
CTACCGATCGAGTGTTTAGGTGAATTTGATGGACGTAAGGAAGTCAAATA19060.7938590462862308No Hit
TCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG16250.6768210651705797No Hit
CTACCGATTTCGGGTGGTCCGGTGAACCCTTTGGACCGGAGGTATCTTCG14240.5931035057248649No Hit
TCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG14010.5835238844947582No Hit
TCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG12520.521464599134502No Hit
CTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGCCCCGGCATTCGG12390.5160500306131374No Hit
CTACCGATTTCGAGTGGTCCGTTGAATCTTTCGGACTGCGCATAGCTTCG11820.4923092301733078No Hit
GTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACTGGGGAAGCCTTGA11790.49105971436068524No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCACGGCTTCG11460.4773150404218366No Hit
CTACCGATTGAACGGTTTAGTGAGACCCTCGGATTGGTCCCGGTACGGGG11430.476065524609214No Hit
TCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG11210.4669024086499815No Hit
TCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG11210.4669024086499815No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGGCCAGTTCACTT10950.4560732716072522No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCAGT10860.4523247241693844No Hit
CCCTCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG10840.45149171362763596No Hit
CCCCCCTCTTTCCAGAAACATTGCTCATGCGGGTGCTTCCGTAGACTTTG10840.45149171362763596No Hit
CTACCGATTTCGAGTGGTCCGGTGAGCCTTTCGGACTGTGGCGGCCTCGA10740.4473266609188939No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCAGCAATGTTTGGA10680.44482762929364866No Hit
TCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG9950.41442274451983185No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACCGCGGCAATGCTTGGA9930.4135897339780835No Hit
TCCTCCATTAAGAAGAAACCTGGCACACGCAGGCGCTTCAGTAGATTTTG9730.4052596285605994No Hit
TCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG9390.3910984493508765No Hit
CTACCGATTGAACGTTTTAGTGAGGTACTTGGATTGGACCTTCGGAGATG9290.38693339664213455No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGTGTGCCGTCTTG9090.3786032912246504No Hit
TCCCCCATTAAGAAGAAACCTGGCACACGCAGGCGCTTCAGTAGATTTTG8710.3627760909314307No Hit
CCCTCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG8230.34278383792946904No Hit
CCCCCCTCTAAGTCATATTACGAGCCACTCTGGTGGTGCAGTTGATTTAG7860.3273731429071235No Hit
TCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG7780.3240411007401299No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGTGCCTCGA7770.3236245954692557No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGC7640.318210026947891No Hit
CTACCGATTGAATGGTTTAGTGAGACCCTCGGATTTGTCCCAGCAGGGTG7530.3136284689682748No Hit
CCCTCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG7530.3136284689682748No Hit
TCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG7400.30821390044691016No Hit
CTACCGATTGGATTTACTAGTGAGACAATAGGACAGCACTCAAATAGCTT7210.3003003003003003No Hit
CCCCCCATTGTCTAGTATCACAGCACACTCAGGTGGAGCTGTTGATTTAG7140.29738476340418085No Hit
CCCTCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG6890.2869721316323258No Hit
CCCTCCATTAAGAAGAAACCTGGCACACGCAGGCGCTTCAGTAGATTTTG6830.2844731000070806No Hit
TCCCCCTCTCTCTAGTAATATTGCACACGCGGGAAGTTCTGTAGACTTTG6740.28072455256921275No Hit
CTACCGATTGGACGTTTTAGTGAGACATTTGGACTGGGTCAGAGTAGTCG6620.2757264893187223No Hit
CGACCGATTGTTCCAGTTAATGAGCGATATGGATGAGTCCCGTGATCGGC6590.27447697350609973No Hit
CTACCGATTGAATGGTTTAGTGAGATCTTCGGATTGCTGGCTCGGCGGCC6570.27364396296435134No Hit
TCCTCCTCTCTCTAGTAATATTGCACACGCGGGAAGTTCTGTAGACTTTG6520.27156143660998033No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGAATGCTGAG6360.26489735227599304No Hit
CCCCCCATTAAGAAGAAACCTGGCACACGCAGGCGCTTCAGTAGATTTTG6230.2594827837546284No Hit
TCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG6190.2578167626711316No Hit
TCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG6190.2578167626711316No Hit
CCCCCCATTATCTAGTGGTAATTCTCATTCAGGTCCTGCGGTAGATTTAG5940.24740413089927651No Hit
CTACCGATTGAATGGTCCGGTGAAGTGTTCGGATAGCGGCAAGGCGGACG5820.24240606764878608No Hit
TCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG5810.24198956237791192No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCGCGGCTCGTCT5790.2411565518361635No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGTGCGTGGTCTCG5760.23990703602354088No Hit
CTACCGATTGAGTGGTTTAGTGAGGCTCACGGACCGCTGGCGGCACCCGG5330.22199730937595016No Hit
CTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTGTTGCGTGTCGTA5330.22199730937595016No Hit
CTACCGATTGAATGTTTCGGTGAAGATTCCGGACTGTGGCTCGCGTGCTT5260.2190817724798307No Hit
CCCCCCTCTCTCTAGTAATATTGCACACGCGGGAAGTTCTGTAGACTTTG5100.21241768814584347No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTCTCCTT5100.21241768814584347No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGATGAGTTTCCTT5070.21116817233322085No Hit
CTACCGATTGAGTGATCCGGTGAACTTTTTGGACGTTGGCGTATGCTGAG4940.20575360381185626No Hit
CATCTTAGTTGTGGGTGGGTGAGGCTACCTCTCTATGGGGTATTCGAGCC4930.20533709854098203No Hit
CTACCGATTTCGAGTGGTCCGGTGAAGCTTTTGGACTGCGCGTGGCTTCG4920.20492059327010784No Hit
CCCTCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4880.203254572186611No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGATAGGAGGGC4850.2020050563739884No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACCGCTTCCTTTGGACGG4750.1978400036652464No Hit
CTAGCGATTGAATGGTCCGGTGAGGCCTCGGGATTGTGGCCCCCGCCATT4740.19742349839437218No Hit
CTACCGATTGAATGGTCCGGTGAGGACTCGGGATTGTGGTTTAGCTCCTT4720.19659048785262379No Hit
CCCCCCACTAAGTCATATTACTAGTCACTCAGGAGGTGCTGTAGATTTAG4640.19325844568563014No Hit
CTACCGATTTCGAGTGGTCCGGTGAACCCTTTGGACCGAGAGCGTCTCGT4510.1878438771642655No Hit
CAACTGATTGGATGACTCGGTGAAAAATTGGGACTGTGAAGAGACCTGCT4290.17868076120503304No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGCTTGGT4290.17868076120503304No Hit
CCCTCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG4290.17868076120503304No Hit
CATCTTAGTTGGGGGTGGGTGAGGCTGCGCTTTATGGCGTATTCGAGCCT4270.17784775066328465No Hit
CCCCCCATTAAGTCATATTACAAGTCACTCTGGAGGTGCAGTAGATTTAG4220.17576522430891361No Hit
CTACCGATTGAATGGTCCGGTGAAATCTTCGGATTGCTGGGTAGGACTTC4210.17534871903803945No Hit
CTACCGATTGAACGTGCTGGTGAAGCGTTAGGACTGGAACTTCGCGCAGG4060.1691011399749264No Hit
CTACCGATTGAATGGTTTAGTGAGATCTCCGGATTGGCGCCATCGCGGCT4050.16868463470405218No Hit
CCTCCGATAGAATGGCTAAGCGAGGCCGTTGGACTGGGCCTGCTTTCGAG3730.15535646603607767No Hit
CTACCGATTGAACGGTTTAGTGAGGGCCTCGGATTGGTCTCGGCCCGCCC3680.1532739396817067No Hit
TCCTCCTCTTAGGTCAACCCTAGGCCATGCCGGGTCATCTGTTGACTTTG3610.15035840278558724No Hit
TCCCCCTCTTAGGTCAACCCTAGGCCATGCCGGGTCATCTGTTGACTTTG3590.14952539224383884No Hit
CCCTCCTCTCTCTAGTAATATTGCACACGCGGGAAGTTCTGTAGACTTTG3580.14910888697296465No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGACTGCGCGCGGCCTCG3500.145776844805971No Hit
CTACCGATTGAATGGTCCGGTGAAGCGTTCGGACTATGACTCTCTGACGG3460.1441108237224742No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTCGGAGAGTAGCGTACCTTG3360.13994577101373218No Hit
CTACCGATTTGAGTGGTCCGGTGAATCTTTTGGACTGTGCACGGCTTCGT3210.13369819195061913No Hit
CTACCGATTGAGTGGTCCGGTGAGATCTCGGGATTGATGCCTGGTGCCTT3090.1287001287001287No Hit
CCCTCCTCTTAGGTCAACCCTAGGCCATGCCGGGTCATCTGTTGACTTTG3050.1270341076166319No Hit
CTACCGATTTCGAGTGGTCCGGTGAATCTTTTGGACTGCGCAGAGCTTCG3030.1262010970748835No Hit
CTACCGATTGAATGATCCGGTGAATAATTTGGACTGTAGCAAATTCCAGT2960.12328556017876408No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGTGACCAGTGCCTTT2920.12161953909526725No Hit
CTACCGATTGAATGGTCCGGTGAAAACTTCGGACTGTGGCAACGTTGCTT2820.11745448638652521No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGATGCAGTGCTCAGC2790.11620497057390261No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCAGTGTTCGGT2720.11328943367778319No Hit
CTACCGATTGAATGGTCCGGTGAAGCCTCGGGATTGGATTCAATGCCTTT2660.11079040205253798No Hit
CTACCGATTGAATGCTCAGGTGAGCCATTCGGACTTTCATCATGTCGGTT2660.11079040205253798No Hit
CTACCGATTGGATGGTCCGGTGAACTGGTCGGACTGATAATTGTCACTGC2610.10870787569816696No Hit
TCCTCCTTTGAGTAGTGCACAGGCTCACTCTGGACCTGCTGTAGATCTTG2610.10870787569816696No Hit
CCCCCCTCTTAGGTCAACCCTAGGCCATGCCGGGTCATCTGTTGACTTTG2590.10787486515641855No Hit
CTACCGATTGAGTGATCCGGTGAATAATTCGGACTGCAGCATTGTTCAGT2540.10579233880204754No Hit
CTACCGATTGAATGGTTTAGTGAGATCCTCGGATTGGTCCCGGCATGGTG2490.10370981244767652No Hit
CTACCGATTGAATGGTCCGGTGAAGCGCTCGGACCGCGGCCTCTTGACGG2490.10370981244767652No Hit
CTACCGATTGAATGATCCGGTGAGGCCCCCGGACTGGGGCGACGCATTCG2460.10246029663505392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)