FastQCFastQC Report
Tue 22 Apr 2025
Site1m_S102_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1m_S102_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167003
Total Bases31.6 Mbp
Sequences flagged as poor quality0
Sequence length46-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG138648.30164727579744No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG118297.083106291503745No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG86885.202301755058293No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA52113.120303228085723No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG49222.947252444566864No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC49012.934677820158919No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC43582.6095339604677763No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT34472.0640347778183625No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT33201.9879882397322204No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG31161.865834745483614No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG30321.815536247851835No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA27221.6299108399250313No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT25791.544283635623312No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA20691.2388999000017964No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA20211.2101579013550654No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG19121.1448896127614474No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT18941.1341113632689233No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT13210.7910037544235733No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT12630.7562738393921067No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA12540.7508847146458447No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC11420.6838200511368059No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT9700.5808278893193536No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT8920.5341221415184159No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG8290.4963982682945815No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG7750.4640635198170093No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA7420.4443033957473818No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATCAGGTTTAC7380.4419082291934875No Hit
AAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT7070.42334568840080716No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT7030.42095052184691295No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT6820.4083758974389682No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT6590.3946036897540763No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC6460.38681939845392No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT6410.3838254402615522No Hit
CTACGGAAACCTTGTTACGCCTTATCCTTCCTCTAAGTGATATGGCTTAC6190.3706520242151339No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC6140.3676580660227661No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA6120.36646048274581894No Hit
GAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT5800.347299150314665No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC5670.33951485901450873No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT5500.3293354011604582No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA4990.2987970275983066No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT4810.2880187781057825No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATTTAACAGTTTGG4570.2736477787824171No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT4510.27005502895157574No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4460.26706107075920793No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT4290.2568816129051574No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT4260.2550852379897367No Hit
GAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT4180.2502949048819482No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4090.24490578013568617No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC4070.24370819685873907No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4030.24131303030484483No Hit
AAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT3910.23412753064316208No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA3750.22454686442758515No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA3590.21496619821200816No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA3510.21017586510421968No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT3370.20179278216558982No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACTGAGTTTGA3370.20179278216558982No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3350.20059519888864275No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAG3130.18742178284222438No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT3130.18742178284222438No Hit
GAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT3090.18502661628833014No Hit
AAATCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT3070.18382903301138304No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATAAGGTTCAG3040.18203265809596234No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG2800.1676616587725969No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA2560.15329065944923145No Hit
GAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT2510.15029670125686365No Hit
GAATCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT2500.1496979096183901No Hit
GAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT2500.1496979096183901No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTCGG2470.1479015347029694No Hit
AAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT2440.14610515978754873No Hit
AAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2440.14610515978754873No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT2430.14550636814907517No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTACAGTTTGG2430.14550636814907517No Hit
CTACAGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG2420.1449075765106016No Hit
GAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT2370.14191361831823382No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2320.13891966012586598No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAGTGATAAGGTTTAC2270.1359257019334982No Hit
AAACCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT2250.13472811865655107No Hit
GAACCAGAAGAGGTGTTGGTATAAAATAGGGTCTCCTCCTCCACTAGGGT2160.12933899391028902No Hit
AAATCAGAAAAGATGTTGGTAAAGAACGGGATCCCCTCCGCCAACTGGGT2120.12694382735639478No Hit
GAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT2110.12634503571792124No Hit
GAATCAAAATAAATGCTGGTATAAAATTGGGTCACCTCCTCCTGCAGGGT2090.1251474524409741No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTCGA2060.12335107752555344No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGATGATAAGGTTCAC1940.1161655778638707No Hit
AAATCAGAAAAGATGTTGATAAAGAACGGGATCCCCTCCGCCAACTGGGT1920.11496799458692358No Hit
GAATCAAAATAAATGTTGATATAGAATAGGATCTCCACCCCCCCCCACAT1920.11496799458692358No Hit
AAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT1840.11017766147913512No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGATCAAGTTCGG1820.108980078202188No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGGTTTGG1790.1071837032867673No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGCTCAC1780.10658491164829374No Hit
AAATCAGAATAAGTGTTGGTATAGAATAGGATCCCCTCCTCCAGTAGGGT1780.10658491164829374No Hit
GAATCAGAACAAATGCTGGTATAGCACAGGATCTCCTCCCCCCGCAGGAT1740.1041897450943995No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAA1700.10179457854050525No Hit
GAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT1690.10119578690203171No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT1680.10059699526355813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)