FastQCFastQC Report
Tue 22 Apr 2025
Site1k_S100_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSite1k_S100_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115533
Total Bases22.9 Mbp
Sequences flagged as poor quality0
Sequence length48-281
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG1833615.870790163849291No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTGG48844.227363610397029No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCTACAGTTTGG43813.7919901673115044No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTTGG42723.6976448287502275No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAA39433.412877705936832No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAC32232.789679139293535No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGATTTGG30512.6408039261509697No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTAC25412.199371608111968No Hit
GAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT21971.9016211818268376No Hit
GAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT17551.5190465061930356No Hit
AAATCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT15751.3632468645322116No Hit
GAATCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT14001.2117749906952993No Hit
GAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT12401.0732864203301222No Hit
AAATCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT12281.0628997775527338No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGCAACAGTTTGG10200.8828646360780037No Hit
AAATCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT10140.8776713146893095No Hit
GAACCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT9940.8603602433936625No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTTAG9900.8568980291345331No Hit
GAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT9830.8508391541810566No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAA9500.8222758865432387No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGA9360.8101581366362857No Hit
GAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT8450.7313927622410913No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGCTCAGGTTTAT8350.7227372265932677No Hit
AAACCAAAATAAATGTTGGTATAACACTGGATCACCTCCTCCAGCAGGAT8200.7097539231215324No Hit
GAACCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT7860.6803251019189323No Hit
AAATCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT7040.609349709606779No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGA7030.6084841560419966No Hit
AAACCAAAATAAATGCTGGTACAGCACTGGATCTCCTCCCCCAGCCACGT7030.6084841560419966No Hit
GAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT6540.5660720313676612No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAA6460.5591476028494023No Hit
AAATCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT5940.5141388174807198No Hit
AAACCAAAATAAGTGTTGATAGAGAATAGGGTCTCCACCACCTCTAGCAT5730.49596219262029034No Hit
GAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT5260.4552811750755195No Hit
GAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT5100.44143231803900185No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGACGCTCAAGTTTGA5040.4362389966503077No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACGCTCAAGTTCGG4980.4310456752616136No Hit
AAATCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT4560.39469242554075457No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATTAGGTTTAC4330.3747846935507604No Hit
AAACCAAAAAAGGTGCTGGAACAAAATAGGGTCACCTCCGCCCGCCGGAT3760.32544814035816605No Hit
GAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT3700.3202548189694719No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCGG3660.3167926047103425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3650.31592705114556013No Hit
GAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT3550.30727151549773657No Hit
AAACCAGAATAAATGTTGATATAAAATAGGGTCTCCTCCACCACTTGGGT3440.29775042628513065No Hit
GAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT3410.2951537655907836No Hit
GAATCAGAATAAGTGTTGAAATAGAATAGGGTCCCCACCACCTTTTGGGT3360.2908259977668718No Hit
CTACGGAAACCTTGTTACGACTTTTAGTTCCTCTAAGTGATGTGGTTTAC3360.2908259977668718No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAGTGGTAAGGTTTGA3290.28476712281339533No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTAGTAAGATTTGC3260.2821704621190482No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTCGTCGAAGGCAGGCTCGA2850.24668276596297162No Hit
CTACAGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGG2850.24668276596297162No Hit
AAATCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT2710.23456501605601862No Hit
AAACCAGAATAAATGCTGATATAAAATCGGGTCACCACCGCCACTTGGAT2540.21985060545471857No Hit
AAATCAGAATAAGTGTTGAAATAGAATAGGGTCCCCACCACCTTTTGGGT2520.21811949832515384No Hit
GAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT2460.2129261769364597No Hit
GAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT2340.20253953415907142No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGACAAGGTTCAG2250.19474955207603023No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGGCAGGCTCGA2170.18782512355777137No Hit
AAATCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT2140.18522846286342431No Hit
CTACGGAAACCTTGTTACGACTTGTCCTTCCTCTAAGTGATGTGGTTTAC2060.17830403434516545No Hit
GAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT1940.16791739156777716No Hit
GAATCAGAAAAGATGCTGATAAAGAACTGGATCTCCTCCCCCGGCAGGGT1920.16618628443821246No Hit
GAACCAGAATAAGTGTTGAAATAGAATAGGGTCCCCACCACCTTTTGGGT1810.15666519522560654No Hit
GAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT1710.14800965957778295No Hit
CTACAGAAGCCTTGTTACGACTTCTCCTTCCTCTGAGTGATAAGGTTCAC1710.14800965957778295No Hit
GAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1690.14627855244821825No Hit
GAATCAAAATAAGTGCTGATAAAGAACAGGGTCCCCTCCTCCAGCAGGGT1680.1454129988834359No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCGC1670.14454744531865354No Hit
GAACCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT1640.14195078462430646No Hit
AAATCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT1630.1410852310595241No Hit
AAATCAGAAAAGGTGCTGGTAAAGCACAGGATCTCCTCCCCCGGCTGGGT1630.1410852310595241No Hit
GAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT1610.1393541239299594No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTCGA1530.13242969541170055No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATGTGGTTTAC1520.1315641418469182No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATAAGGTTCAG1510.13069858828213585No Hit
CTACGGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGGTTTAA1490.12896748115257112No Hit
CTACAGATACCTTGTTACGACTTAACCCTCCTTGTCGAAGACAGGCTCGA1480.12810192758778877No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATAAGGTTTAC1470.12723637402300642No Hit
CTACGGAAACCTTGTTACGACTTTTCCTTCCTCTAAGTGATATGGTTTAA1450.12550526689344169No Hit
AAACCAGAATAAGTGTTGAAATAGAATAGGGTCCCCACCACCTTTTGGGT1410.12204305263431227No Hit
AAATCAAAAAAGATGTTGAAATAGCACAGGATCGCCGCCACCAGCTGGGT1390.12031194550474757No Hit
GAACCAAAACAAATGCTGATATAAGACAGGATCACCACCACCGTTTGGAT1350.11684973124561812No Hit
GAACCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT1300.11252196342170634No Hit
AAACCAGAAAAGGTGCTGGTAAAGTACAGGATCTCCTCCCCCTGCTGGGT1300.11252196342170634No Hit
AAATCAAAAAAGATGCTGGTATAAAATAGGATCTCCCCCCCCTCTTGCAT1290.111656409856924No Hit
AAACCAAAACAAATGTTGATATAATACAGGATCTCCTCCCCCAGCAGGAT1260.10905974916257692No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTCAT1230.10646308846822986No Hit
AAATCAGAAAAGATGCTGATAAAGAACTGGATCTCCTCCCCCGGCAGGGT1220.10559753490344749No Hit
TACGGAAACCTTGTTACGACTTTTACTTCCTCTAAACACTACAGTTTGGT1210.10473198133866514No Hit
AAATCAAAAAAGGTGTTGGTAGAGTACTGGATCTCCGCCTCCTGCTGGAT1190.10300087420910044No Hit
CTACGGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATGTGGTTTAC1190.10300087420910044No Hit
AAATCAAAATAAGTGCTGATAAAGAACAGGGTCCCCTCCTCCAGCAGGGT1160.10040421351475337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)